The Construction of Biological Computing Platform Based on Multiple-Stimuli Responsive Bienzyme Electrocatalysis

Abstract

Biomolecular logic gate systems and parity checker based on stimuli-responsive film electrode were developed. {CS/Con A} n -PDEA-GOD-HRP films were constructed on pyrolytic graphite (PG) electrode surface by specific recognition, layer by layer assembly of CS (Chitosan) and Con A (Concanavalin A) and chemical polymerization of PDEA-GOD-HRP (PDEA = poly (N,N-diethyl acrylamide), GOD = glucose oxidase, HRP = horseradish peroxidase). The electroactive probe, potassium ferricyanide (K 3 Fe(CN) 6 ), exhibited cyclic voltammograms (CV) switching behaviors to pH, temperature and methanol on film electrodes. This switching property of the {CS/Con A} n -PDEA-GOD-HRP films could be further used to realize multiple responsive catalytic oxidation of glucose sequentially by HRP and GOD entrapped in the films with K 3 Fe(CN) 6 as the electron transfer mediator. The addition of glucose could amplify CV peak current through bioelectrocatalysis. Based on experimental results, a 4-input/5-output binary logic gate system with glucose, pH, temperature and methanol as input and peak current (I pc ) as output was constructed, a ternary INHIBIT logic gate, a ternary AND logic gate and a parity checker were established. It opens up the possibility of building other complex molecular logic devices based on bienzyme bioelectrocatalysis.

Construction of Water Conservancy and Energy Engineering Structure Platform Based on Cloud Computing

“…[7]. From a market-oriented perspective, Wu and others believe that cloud computing is a parallel set of internally interconnected virtual machines, and the distributed computing system can dynamically provide computing resources according to the service level agreement negotiated between the service provider and the customer [8]. In order to get better platform access performance, for the design of the data buffer and database, a more in-depth optimization design is needed.…”

Recent Insights into Glucose-Responsive Concanavalin A-Based Smart Hydrogels for Controlled Insulin Delivery

“…Multilayer films were prepared through a pH-sensitive self-assembling mechanism by using chitosan (CS) and Con A as the inner layer and poly (N,N-diethyl acrylamide) (PDEA) hydrogel as the outer layer that contains glucose oxidase (GOD) and horseradish peroxidase (HRP) enzymes. The resulting {CS/Con A} n -(PDEA-GOD-HRP) multilayer film with a binary structure was fixed on the electrode surface [159], this system being able to control the bio-electro-catalysis of glucose in the presence of enzymes.…”

The Construction of Stimulus‐responsive Film Electrode by the Cu‐catalyzed Radical Polymerization and its Application in Multi‐valued Biologic Systems

“…In previous reports, a general strategy for raising the complexity of logic computing involved coupling various polymers with a single stimulus-response characteristic to form multi-responsive copolymer [20] or semi-interpenetrating polymer films [21]. But, the interference between structures and properties of different polymer might affect the response output.…”