The construction of high-density paraffin tissue microarrays with 0.43-mm-diameter paraffin tissue core biopsies is technically feasible

Vogel, Ulrich

doi:10.1007/s00428-008-0622-9

Cited by 17 publications

(15 citation statements)

References 9 publications

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“…Application of 2 mm punchers allows construction of tissue arrays with about 50–100 specimens [14], while application of 0.6 mm punchers allows construction of a typical array of 300–500 specimens with a spacing of 0.8 mm between sample centers per block in regular tissue cassettes. A recipient block could accommodate up to 1,000 samples since the core of diameter can be as small as 0.43 mm [20]. Removing small diameter cores of tissue means that the tissue in the main block is preserved and does not significantly damage the structure of the tumor in the donor block, which can be used for further conventional sectioning should this become necessary.…”

Section: Discussionmentioning

confidence: 99%

An alternative high output tissue microarray technique

Shi

Hou

et al. 2013

Diagn Pathol

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BackgroundTissue microarray (TMA) is a high throughput research tool, which has greatly facilitated and accelerated in situ tissue analyses. However, its productivity has been restricted due to the confined thickness of traditional donor block. Here, we introduce an improved high output TMA method that is applicable to a broader range of tissue samples.MethodsIn this method, a 3.6 cm long and 2.7 cm wide recipient block with 88 square lattices (3 mm in width) was first prepared using several commercial instruments. A 2 mm wide and 6 mm long tissue rod was then prepared using a self-made blade-shaped knife from each paraffin embedded donor block of gastrointestinal stromal tumors. These rods were manually arrayed one by one into the corresponding lattices of the 60°C pre-softened recipient block with the guide of holes drilled with a steel needle. A 70-rod TMA was made to testify this method.ResultsThe prepared TMA had well defined array configurations, good tissue morphology and fully preserved proteins and DNA. A total of 500–1000 TMA sections could be easily obtained from a TMA block.ConclusionThis low-cost and time-saving method provides an alternative sampling tool for high output TMA.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1979605867857990

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Section: Discussionmentioning

confidence: 99%

An alternative high output tissue microarray technique

Shi

Hou

et al. 2013

Diagn Pathol

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“…;Vogel & Bueltman;Pires et al, 2006;Vogel, 2008;Hidalgo et al, 2003) However in some cases the elaboration requires the use of certain specialized tools, such as grinder and drill. In this study we intentionally avoid the use of electrical tools that could be in some cases difficult to obtain, most of materials used with the exception of cutting pliers are commonly used in various hospitals and offices, this allows a better reproduction of the method described in our study compared with those previously published.…”

Section: Discussionmentioning

confidence: 99%

Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays

2013

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“…Because it is easy to modify the number of cores in the array, it is easy to adapt this to individual labs and requirements. Vogel, [13] described a method that allows the construction of PTMAs with tissue cores only 0.43 mm in diameter in order to conserve the minute quantities of available tumor tissue and to store the greatest possible number of tissue cores in one block. So, we will try to construct PTMAs by the use of mechanical pencil tips with diameter 0.5 mm.…”

Section: Discussionmentioning

confidence: 99%

An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips

et al. 2011

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BackgroundTissue microarray technology has provided a high throughput means of evaluating potential biomarkers in archival pathological specimens. This study was carried out in order to produce tissue microarray blocks using mechanical pencil tips without high cost.MethodConventional mechanical pencil tips (Rotring Tikky II Mechanical Pencil 1.0 mm) were used to cut out 1 mm wax cylinders from the recipient block, creating from 36 to 72 holes. Three cores of tumor areas were punched out manually by using the mechanical pencil tips from donor paraffin embedded tissue blocks and transferred to the holes of the paraffin tissue microarrays.ResultsThis technique was easy and caused little damage to the donor blocks. We successfully performed H&E slides and immunodetection without substantial tissue cylinder loss.ConclusionOur mechanical pencil tip technique is the most inexpensive easy technique among the literature. It also takes a reasonable amount of time and reduces antibody consumption during immunohistochemistry

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The construction of high-density paraffin tissue microarrays with 0.43-mm-diameter paraffin tissue core biopsies is technically feasible

Cited by 17 publications

References 9 publications

An alternative high output tissue microarray technique

An alternative high output tissue microarray technique

Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays

An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips

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