The Construction, Selection, Characterization, and Application of Recombinant Herpes Viruses

Ackermann, Mathias

doi:10.1111/j.1439-0450.1988.tb00510.x

Cited by 3 publications

(2 citation statements)

References 45 publications

(37 reference statements)

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“…This multimutated replication competent oncolytic HSV is built from G207 by the deletion of the α47 gene [35,36]. Owing to overlapping transcripts encoding ICP47 and US11, the deletion has dual functions: 1) enhanced major histocompatibility complex class I presentation because ICP47 inhibits the transporter associated with antigen presentation; and 2) enhanced viral replication due to the late US11 gene being under control of the immediate-early α47 promoter [36].…”

Section: G47δmentioning

confidence: 99%

Oncolytic Virotherapy for the Treatment of Malignant Glioma

et al. 2017

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Malignant glioma is the most common primary brain tumor and carries a grim prognosis, with a median survival of just over 14 months. Given the poor outcomes with standard-of-care treatments, novel treatment strategies are needed. The concept of virotherapy for the treatment of malignant tumors dates back more than a century and can be divided into replication-competent oncolytic viruses and replication-deficient viral vectors. Oncolytic viruses are designed to selectively target, infect, and replicate in tumor cells, while sparing surrounding normal brain. A host of oncolytic viruses has been evaluated in early phase human trials with promising safety results, but none has progressed to phase III trials. Despite the 25 years that has passed since the initial publication of genetically engineered oncolytic viruses for the treatment of glioma, much remains to be learned about the use of this therapy, including its mechanism of action, optimal treatment paradigm, appropriate targets, and integration with adjuvant agents. Oncolytic viral therapy for glioma remains promising and will undoubtedly impact the future of patient care.

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Section: G47δmentioning

confidence: 99%

Oncolytic Virotherapy for the Treatment of Malignant Glioma

et al. 2017

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“…Herpesviruses are among the most common virus types used as viral vectors for the expression of foreign genes (Ackermann 1988;Heffner, Kovacs, Klupp & Mettenleiter 1993). The major emphasis of this research has been the development of viral vaccine vectors.…”

Section: Introductionmentioning

confidence: 99%

Recombinant channel catfish virus (Ictalurid herpesvirus 1) can express foreign genes and induce antibody production against the gene product

Zhang

Hanson

1996

Journal of Fish Diseases

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The potential use of channel catfish virus (CCV) [fctalurid herpesvirus 1) as a vaccine vector for the channel catfish industry was investigated by inserting the Escherichia coli lacZ gene into the CCV genome and evaluating the immune response to the foreign gene product in catfish exposed to the recombinant. The recombinant virus was produced by inserting the lacX in reading frame with the ATG start codon of the CCV thymidine kinase (TK) gene in the recotnbinanr transfer plasmid pBSCV437 described previously. The plasmid was then cotransfccted with CCV DNA in aTK gene-mediated selectable homologous recombination. The recombinant progeny were selected by resistance to 0-1 mM acycloguanosinc (acyclovir) and the production of blue plaques in the presence of 5-bromo-4-chloro-3-indolyl-^-Dgalactopyranoside (X-gal). The resultant construct (CCVlacZ) was TK^, and contained the lacZ gene at both TK loci in the genome. jS-Galactosidase expression in infected CCO cells reached 0-33 /ig per 10'' CCO cells at 12 h post-infection. When channel catfish fingerlings were immersion exposed to CCVlacZ, these developed an antibody response to the inserted foreign gene product which peaked at approximately 13-20 days post-infection. Additionally, the anti-^galactosidase response was significantly enhanced when the fmgerlings were re-exposed to the virus 20 days after the initial exposure. These results demonstrate that foreign genes can be inserted into and expressed by CCV and that such constructs could be used as vaccine vectors.

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