Prejudice against people with Vitiligo is pervasive across societies. However, little attention has been given to measuring individual differences on the way that it is expressed. To overcome this gap, we developed and validated the Scale of Prejudice towards People with Vitiligo (SPV) throughout six online studies. Studies 1a and 1b (N = 136) addressed the development of the SPV's items, their content and semantic validity. Study 2 (N = 121) showed that its nine items are organized into three correlated factors (cognitive, behavioral, and affective) with high internal consistency. Studies 3a and 3b (N = 386) confirmed this factor's structure using confirmatory factor analysis, and explored the convergent-discriminant validity of the instrument. Study 4 (N = 417) replicated these findings and demonstrated the SPV's configural, metric, and scalar invariance between two experimentally manipulated target-groups: Black person with Vitiligo versus White person with Vitiligo. Results also showed experimental criterion validity, in that participants scored higher on SPV in the Black (vs. White) Vitiligo condition. Furthermore, results revealed that the higher the social contact with people with Vitiligo, the lower the prejudice expressed towards this population. When combined, these findings are the first to document the correlational and experimental evidence of a measure of prejudice towards individuals with Vitiligo.