The content and profile of biologically active compounds present in individual parts of nasturtium (Tropaeolum majus L.): comprehensive study

Česlová, Lenka; Klikarová, Jitka; Šalomounová, Tereza

doi:10.1007/s00217-022-04126-4

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…TAC assessment: The preparation of buffer solutions was adopted from [ 35 ]. An amount of 200 μL of elderberry extract was added to 2.8 mL of buffers with pH 1 and pH 4.5, and their absorbance was recorded at wavelengths 520 and 700 nm.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Sample Pretreatment on the Anthocyanin Content in Czech Wild Elderberry (Sambucus nigra L.)

Česlová,

Kalendová,

Dubnová

et al. 2023

Molecules

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This work focusses on the analysis of anthocyanins present in wild Czech elderberries, using spectrophotometric methods and liquid chromatography. Spectrophotometric methods were used to determine antioxidant capacity, total phenolic content, and total anthocyanin content. Further, four major elderberry anthocyanins were determined using reversed-phase high-performance liquid chromatography with isocratic elution of 30% aqueous methanol with 5% of formic acid. All optimised methods were applied to the analysis of extracts prepared from frozen and dried elderberry fruit samples, and the results were evaluated using principal component analysis which clearly divided the samples into individual groups according to the sample pretreatment (frozen and dried samples). The frozen samples reached higher values of antioxidant capacity and total phenolic and anthocyanin contents compared to the dried samples, probably due to the degradation of anthocyanins during the drying process.

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Section: Methodsmentioning

confidence: 99%

The Effect of Sample Pretreatment on the Anthocyanin Content in Czech Wild Elderberry (Sambucus nigra L.)

Česlová,

Kalendová,

Dubnová

et al. 2023

Molecules

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Section: Introductionmentioning

confidence: 99%

“…The broad therapeutic spectrum of T. majus can be linked to a group of compounds known as glucosinolates [15][16][17][18]. Benzyl glucosinolate is a metabolite found in every part of the T. majus plant, especially in the seeds [15][16][17][18][19]. When hydrolyzed by the endogenous enzyme myrosinase (Thioglucoside hydrolase, EC 3.2.3.1), it generates a variety of breakdown products, including benzyl isothiocyanate (BITC) [16,17,19], which has been reported to have antimicrobial [20][21][22][23], larvicidal [24], anthelmintic [25], and anticancer [26] activities.…”

Section: Introductionmentioning

confidence: 99%

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

Pintão,

Santos,

Nogueira

2024

Preprint

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Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of BITC and T. majus seeds, leaves, and stems aqueous extracts. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of chloroquine-susceptible Plasmodium falciparum (P. falciparum) 3D7-GFP strain. Extracts and/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7-GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity higher in seeds than in leaves or stems. BITC’s GI was comparable to chloroquine’s. BITC’s IC50 was similar in both strains, thus a cross-resistance absence with aminoquinolines was found (RI < 1). BITC presented features that could open new avenues for malaria drug discovery.

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“…The broad therapeutic spectrum of T. majus can be linked to a group of compounds known as glucosinolates [15][16][17][18]. Glucosinolates are stable water-soluble precursors of isothiocyanates located in vacuoles [19].…”

Section: Introductionmentioning

confidence: 99%

“…The benzyl glucosinolate metabolite is found in every part of the T. majus plant, especially in the seeds [15][16][17][18]20]. When hydrolyzed by the endogenous enzyme myrosinase (Thioglucoside hydrolase, EC 3.2.3.1), it generates a variety of breakdown products, including benzyl isothiocyanate [19].…”

Section: Introductionmentioning

confidence: 99%

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

Pintão,

Santos,

Nogueira

2024

Molecules

View full text Add to dashboard Cite

Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of aqueous extracts of BITC and T. majus seeds, leaves, and stems. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of the chloroquine-susceptible Plasmodium falciparum 3D7 − GFP strain. Extracts and/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7 − GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity that was higher in seeds than in leaves or stems. BITC’s GI was comparable to chloroquine’s. BITC’s IC50 was similar in both strains; thus, a cross-resistance absence with aminoquinolines was found (RI < 1). BITC presented features that could open new avenues for malaria drug discovery.

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The content and profile of biologically active compounds present in individual parts of nasturtium (Tropaeolum majus L.): comprehensive study

Cited by 4 publications

References 24 publications

The Effect of Sample Pretreatment on the Anthocyanin Content in Czech Wild Elderberry (Sambucus nigra L.)

The Effect of Sample Pretreatment on the Anthocyanin Content in Czech Wild Elderberry (Sambucus nigra L.)

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

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