The contractile effects of endothelins on isolated isthmic segment
of human oviduct at the luteal phase of the menstrual cycle

Sm, Janković; Sv, Jankovic; Lukic, Goran; Čanović, Dragan; Folić, Marko

doi:10.1358/mf.2010.32.2.1428740

Cited by 6 publications

(4 citation statements)

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“…On the other hand, EDN1 and EDN2 trigger a concentrationdependent contraction of the human isthmic segment of the oviduct during the luteal phase. (Jankovic et al 2010) In general, it is thought that endothelins have a conserved Figure 3 EDN2 triggers the contraction of mature follicles in the ovary. Prior to follicular rupture, a follicle rapidly grows without the support of an accompanying vascular oxygen supply.…”

Section: Endothelin In the Human Ovarymentioning

confidence: 99%

Endothelin 2: a key player in ovulation and fertility

Cho

Ham

et al. 2022

Reproduction

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Ovulation is the fundamental biological process during which an oocyte is expelled from the ovary, and it is an essential step toward establishing pregnancy. Understanding regulatory mechanisms governing the ovulation process is essential for diagnosing and treating causes of infertility, identifying contraceptive targets, and developing novel contraception methods. EDN2 is a 21-amino acid long peptide that is transiently synthesized by granulosa cells of the ovulatory follicle prior to ovulation and plays an essential role in ovulation via promoting contraction in the myofibroblast cells of the theca layer of the follicle. This review describes the organization of the endothelin system, summarizes recent findings on the expression and synthesis of the endothelin system in the ovary, illustrates the roles that EDN2 plays in regulating ovulation, and discusses EDN2 as a potential target of contraception.

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Section: Endothelin In the Human Ovarymentioning

confidence: 99%

Endothelin 2: a key player in ovulation and fertility

Cho

Ham

et al. 2022

Reproduction

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“…The mRNA levels of Rasd1 ( p <0.0001 for both time points) and Edn1 ( p = 0.043 and p = 0.012) were also significantly increased at both time points. EDN1 is known to be involved in muscular contraction of the human and bovine fallopian tube [ 12 – 14 ], and thus, the two other isoforms of endothelin ( Edn2 and Edn3 ) and its receptors ( Ednra and Ednrb ) were included in the analysis even though they were not identified as regulated by the microarray. We also included Pgr in the analysis because earlier studies have shown a regulation by progesterone in the fallopian tube [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…The absence of apparent regulation at 20 min suggests that progesterone-activation of the endothelin system via gene transcription is not a prerequisite for regulating ciliary function, but its regulation at later time points in the fallopian tube suggests that the endothelin system could be involved in other processes related to gamete transport. Indeed, EDN1 is known to induce muscle contractility in the fallopian tube [ 11 , 12 , 43 ]. Earlier studies show that estrogen and estrogen in combination with progesterone and luteinizing hormone significantly stimulates the production and secretion of EDN1 from bovine oviduct epithelial cells in culture.…”

Section: Discussionmentioning

confidence: 99%

Progesterone-mediated effects on gene expression and oocyte-cumulus complex transport in the mouse fallopian tube

Bylander

Gunnarsson

Shao

et al. 2015

Reprod Biol Endocrinol

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BackgroundThe fallopian tube transports the gametes to the fertilization site and delivers the embryo to the uterus at the optimal time for implantation. Progesterone and the classical progesterone receptor are involved in regulating both tubal ciliary beating and muscular contractions, likely via both genomic and non-genomic actions.MethodsTo provide more details of the underlying mechanisms, we investigated the effect of progesterone on gene expression in mice fallopian tubes in vitro at 20 min, 2 h and 8 h post progesterone treatment using microarray and/or quantitative PCR. In parallel, oocyte cumulus complex transport was investigated in ovulating mice that were injected with one of the progesterone receptor antagonists, Org 31710 or CDB2194.ResultsMicroarray analyses did not reveal any apparently regulated genes 20 min after progesterone treatment, consistent with the proposed non-genomic action of progesterone controlling ciliary beating. After 2 h, 11 genes were identified as up-regulated. Analyses using quantitative PCR at 2 h and 8 h showed a consistent up-regulation of endothelin1 and a down-regulation of its receptor Endothelin receptor A by progesterone. We also confirmed that treatment with progesterone receptor antagonists before ovulation accelerates the transport of the oocyte cumulus complex.ConclusionsThis is the first study showing that progesterone regulates the expression of endothelin1 and endothelin receptor A in the fallopian tube. Together with previous studies of the effects of endothelin on muscular contractions in the fallopian tube, the results from this study suggest that endothelin is a mediator of the progesterone-controlled effects on muscular contraction and eventually gamete transport in the fallopian tube.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-015-0038-8) contains supplementary material, which is available to authorized users.

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“…Our present study showed that GPER1 is expressed on the oviductal epithelium (Figure ), and E2 rapidly stimulates EDN2 mRNA expression mediated by GPER1 in the isthmus (Figures 3–5). It was previously determined that EDN2 promotes oviductal contraction in humans and rats (Al‐Alem et al., ; Jankovic, Jankovic, Lukic, Canovic, & Folic, ). Based on our findings, a high concentration of E2 contained in follicular fluid seems to contract the isthmus by promoting EDN2 secretion mediated by GPER1 in the isthmic epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Acute stimulation of a smooth muscle constrictor by oestradiol‐17β via GPER1 in bovine oviducts

Nishie

Kobayashi

Kimura

et al. 2017

Reprod Domestic Animals

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Oviducts play roles in reproductive processes, including gametes transport, fertilization and early embryo development. Oviductal transport is controlled by various factors such as endothelins (EDNs) and nitric oxide (NO), smooth muscle contracting and relaxing factor, respectively. EDNs and NO production depend on an ovarian steroid hormone, oestradiol-17β (E2) and E2 quickly exerts their biological functions through G protein-coupled oestrogen receptor 1 (GPER1), which mediates rapid intracellular signalling. Because follicular fluid which contains a high concentration of E2 enters the oviduct, we hypothesized that E2 in the follicular fluid participates via GPER1 in producing EDNs and NO. To test this hypothesis, we investigated 1) the expression and localization of GPER1 in bovine oviductal tissues and 2) rapid effects of E2 via GPER1 on EDN1, EDN2 and inducible NO synthase (iNOS) expression in cultured bovine oviductal isthmic epithelial cells. GPER1 was observed in the oviductal epithelium, stroma and smooth muscle, and its expression was highest in the isthmus. Short-term treatments (≤1 hr) of E2 increased EDN2 mRNA expression in the isthmic epithelial cells, although E2 did not affect EDN1 and iNOS mRNA expressions. Results of GPER1-selective agonist G-1 and GPER1-selective antagonist G-15 treatments revealed acute stimulation by E2, which is mediated via GPER1. The overall findings suggested that E2 in follicular fluid rapidly stimulates EDN2 expression via GPER1 in the isthmic epithelial cells. Follicular fluid may play a role in retention of the ovulated oocyte in the end of ampulla by contracting the isthmus for successful fertilization.

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The contractile effects of endothelins on isolated isthmic segment of human oviduct at the luteal phase of the menstrual cycle

Cited by 6 publications

References 0 publications

Endothelin 2: a key player in ovulation and fertility

Endothelin 2: a key player in ovulation and fertility

Progesterone-mediated effects on gene expression and oocyte-cumulus complex transport in the mouse fallopian tube

Acute stimulation of a smooth muscle constrictor by oestradiol‐17β via GPER1 in bovine oviducts

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