Introduction: Lateral ankle sprain (LAS) is a common musculoskeletal injury among Canadian Armed Forces (CAF) members that challenges their physical readiness. Th e operational impact of LAS on the CAF may be minimized through evidence-based practice (EBP). Th is research project delivered a knowledge translation (KT) intervention tailored for CAF physiotherapists to improve their knowledge and performance of EBP in LAS management. Methods: All CAF physiotherapists ( n = 67) were sent an invitation to complete an online questionnaire investigating their self-reported knowledge and performance of interventions and outcomes recommended in the management of LAS. One three-hour, active, multi-component KT intervention, including summarized research, point-of-care practice tools, and peer interaction, was then facilitated over a distance learning platform. Th e primary outcome was the median change reported on the online questionnaire before and six months aft er the KT intervention. Results: Th e response rate to the online questionnaire at six months was 64.2% ( n = 43). Respondents reported improvements in their knowledge and performance of recommended rehabilitation interventions and outcome measures between baseline and six months. Some discrepancies between respondents' knowledge and performance of recommended outcome measures remained at six months. Unfamiliarity, use of competing outcome measures, or restraints in clinical settings explained these discrepancies, which may require changes at the clinical setting or organization level. Discussion: CAF physiotherapists improved their self-reported knowledge and performance of rehabilitation interventions and outcome measures recommended in LAS management six months aft er participation in an active, multi-component KT intervention delivered through distance learning. Further adoption of EBP may require changes at the clinical setting or organization level. Th ese results should inform KT interventions directed at improving EBP by CAF physiotherapists and, conceivably, other CAF Health Services Centre providers.