Introduction: With regard to the wide-ranging effects of gender dysphoria disorder on the social and mental performances, as well as the little awareness about this disorder in Iran, inappropriate and irrational behaviors are expected due. Therefore, the aim of this study was to determine the difference between personality traits of people with gender dysphoria disorder and those of healthy subjects using the Rorschach test. Materials and Methods: Twenty-eight patients with gender dysphoria disorder and 28 healthy subjects using available sampling method were selected and evaluated. Then, subjects were individually evaluated after being selected by Rorschach test; based on Axner's comprehensive system. Finally, the data were analyzed using t-test analyzing method in SPSS 21 software. Results: The results showed that people with gender dysphoria disorder and healthy people had a significant difference in some specific variables (YF, FT, F, FQo, FQu). So that; in some variables (M, YF, FT), patients with gender dysphoria disorder got higher scores, but in some other variables (F, FQo, FQu), healthy subjects got higher scores. Conclusion: Those with gender dysphoria disorder had mental illness, feelings of helplessness, isolation, and an attempt to create a gap between themselves and the environment and the expression of suppressed excitement which are directly associated with experience of depression and anxiety.