The contribution of glucose and glutamine to energy metabolism in newborn pig enterocytes

Posho, Leta; Darcy‐Vrillon, Béatrice; Blachier, François; Duée, Pierre‐Henri

doi:10.1016/0955-2863(94)90033-7

Cited by 16 publications

(18 citation statements)

References 29 publications

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“…Lactate is a significant oxidative fuel in some tissues (Sauer et al, 1982;Paglissotti and Donovan, 1990;Berry et al, 1992) and is oxidized by platelets in the absence of significant levels of other fuels . There could also be a contribution from endogenous fuels, the significance of which has been mooted for 30 years (Racker, 1965;Posho et al, 1994), but never seriously investigated. Platelets do have stores of glycogen, triacylglycerol, free fatty acids and amino acids, but our preliminary studies (Niu, X., unpublished results) are equivocal on the utilization of these fuels.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly it is now obvious that aerobic glycolysis contributes significantly (20 -50%) to ATP production under aerobic conditions in a variety of cell types (reviewed in Neermann and Wagner, 1996;Guppy et al, 1993) so lactate production must be measured if one is to get a meaningful measure of total ATP turnover. Studies in which comprehensive energy budgets were the primary aim, and in which there was a measurement of total ATP turnover (oxygen consumption and lactate production) are not common, but do exist for human platelets (Guppy et al, 1990), rat thymocytes (Guppy et al, 1993), pig enterocytes (Posho et al, 1994), mouse erythroid cells (Kim et al, 1992), human and rat lymphocytes (Roos and Loos, 1973;Ardawi and Newsholme, 1985), fish erythrocytes (Sephton et al, 1991) and rat coronary endothelial cells (Noll et al, 1990). These studies provide information on total ATP turnover, contributions by oxidative and glycolytic components, and the contributions to the oxidative component by various oxidative fuels such as glucose, acetate and glutamine.…”

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confidence: 99%

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Fuel Choices by Human Platelets in Human Plasma

Guppy

Abas

Neylon

et al. 1997

European Journal of Biochemistry

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Despite the fact that homogeneous preparations of isolated cells are now being used very effectively to study a range of important biochemical questions, it is still not known what combination of fuels and energy-producing pathways is used by cells when offered the complex mixture characteristic of plasma or extracellular fluid. We have developed an in vitro system whereby highly purified and functional human platelets are incubated in human plasma that has been minimally modified from its native state. The concentration of platelets and fuels, and the complexity of fuels in the incubation are similar to those in vivo. The preparation thus represents a reasonable approximation of the physiological condition, considering the complex nature of the system being studied. Measurements carried out simultaneously during the incubation are rates of oxygen consumption, lactate production and fuel oxidation. The data allow the calculation of total ATP turnover, and contributions to this turnover by lactate production and the oxidation of individual fuels. Lactate production accounts for 24% of the ATP turnover. The oxidation of glucose and 3-hydroxybutyrate each account for under S%, palmitate for 21 %, oleate for 7% and acetate for 9%, leaving 32% of the ATP turnover as yet unaccounted for. The results confirm some previous measurements in the literature, but show that data collected under non-physiological experimental conditions can be misleading.Keywords: platelet; oxygen consumption ; plasma; glycolysis ; oxidation.The preparations used for the study of cellular metabolism have progressed over the last 40 years from tissue homogenates and slices to homogeneous preparations of isolated cells. The latter systems are now being used very effectively to study a range of important biochemical questions. However, there is still a question that has never really been adequately addressed, which could have important implications for the relevance of these preparations, and which is fundamental to our understanding of cell biology. This question concerns the combination of fuels and energy-producing pathways used by a cell when it is offered the complex mixture characteristic of plasma or extracellular fluid.Where energy budgets have been presented previously, it is often the case that total ATP turnover has been underestimated due to the omission of any measurement of oxygen consumption. This approach results in overestimates of the contributions of particular fuels and pathways to total ATP turnover as the 'total ATP turnover' figure is too low. For example Petch and Butler (1994) and Neermann and Wagner (1996) conclude that the various pathways of glucose and glutamine utilization account for 100% of ATP turnover in various cell lines in the presence of glucose, glutamine and fetal calf serum. Oxygen consumption was not measured and would almost certainly demonstrate the oxidation of other fuels. Similarly it is now obvious that aerobic glycolysis contributes significantly (20 -50%) to ATP production under aerobic conditions...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Fuel Choices by Human Platelets in Human Plasma

Guppy

Abas

Neylon

et al. 1997

European Journal of Biochemistry

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“…In the experiment of Vente-Spreeuwenberg et al (2003) glucose and lactose did not affect growth performance and villus architecture. According to Posho et al (1994) the capacity of enterocytes to utilize glucose increased threefold between birth and 2 days of age. Fleming et al (1991) found that cells taken from the jejunum utilized exogenous substrates in decreasing order glutamine > glucose > butyrate.…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary glutamine, glucose and/or sodium butyrate on piglet growth, intestinal environment, subsequent fattener performance, and meat quality

Hańczakowska

Niwińska

Grela³

et al. 2014

CZECH J ANIM SCI

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ABSTRACT:The effect of feed supplementing with glutamine, glucose and/or sodium butyrate was estimated on 156 piglets. The after-effect of supplements on fattener performance, carcass traits, and meat quality was examined. Piglets were allocated to 5 groups and fed standard feed mixture alone (control -C) or supplemented with 10 g of l-glutamine, or 10 g of glucose, or 3 g of sodium butyrate per kg of diet or all of these compounds (groups GT, GC, SB, and GT+GC+SB, respectively). Six piglets from each group were slaughtered at 63 days of age, their intestines were prepared and their parts measured. Digesta from ileum and caecum was taken for analysis. Its acidity and volatile fatty acids content were evaluated. Morphological structure of duodenal and ileal epithelium was estimated. After 84 days of age 20 animals from each group were fed the standard mixture. After 100 days of fattening 8 pigs from each group were slaughtered, pH of meat was measured, and samples of the longissimus muscle were taken for analysis. Body weight gains of piglets fed diets supplemented with SB or all supplements were higher than those of controls. All supplements given together increased total intestinal weight and length. Epithelial villi in jejunum were the highest in piglets receiving all supplements. Their height in the duodenum ranged from 296 to 347 µm and in the jejunum they were higher: 336 -424 μm. After 100 days of fattening body weight of all experimental animals was higher than that of control. There was no significant difference in carcass and meat quality. Sodium butyrate added to the diet improved piglet performance probably due to changes in intestine development and in intestinal epithelium structure. This positive effect was enhanced to some extent by the addition of glutamine or glucose.

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“…This is easily accounted for by triglycerides from the FCS, provided there is appropriate lipase activity. There could also be a contribution fkom endogenous fuels, the presence of which has been noted for 30 years (Racker, 1965;Posho et al, 1994) but never seriously investigated. Endogenous fuels could differ quantitatively from those in the culture medium and could easily be quantitatively significant.…”

Section: Discussionmentioning

confidence: 96%

“…Studies in which comprehensive energy budgets were the primary goal and in which there was a measurement of total ATP turnover (oxygen consumption and lactate production) are not common but do exist for platelets (Guppy et al, 1990), thymocytes (Guppy et al, 1993), pig enterocytes (Posho et al, 1994), erythroid cells (Kim et al, 1991), lymphocytes (Roos and Loos, 1973;Ardavvi and Newsholme, 1985), fish erythrocytes (Sephton et al, 1991), and coronary endothelial cells (No1l et al, 1990). These studies provide information on total ATP turnover, contributions by oxidative and glycolytic components, and the contributions to the oxidative component by various fhels such as glucose, acetate, and glutamine.…”

mentioning

confidence: 98%

Method for measuring a comprehensive energy budget in a proliferating cell system over multiple cell cycles

et al. 1997

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Isolated cell systems are now being used very effectively to study a range of important biochemical questions, but their energy metabolism has never been comprehensively investigated. We have developed a system, using J2E cells, which enables us to measure total ATP turnover and the contribution of various fuels and pathways to this total in a dynamic, proliferating preparation. Cells are cultured in 500 ml airtight glass containers which enables (1) the measurement of oxygen consumption, (2) the collection and measurement of 14CO2 production from labelled fuels, and (3) the measurement of metabolite utilization and production. Data on cell numbers are then used to produce a curve of cell number vs. time, the area under which (cell numbers x hour) is used as a base by which all measurements and experiments are compared. To our knowledge this is the first time a comprehensive energy budget has been measured in a proliferating cell system over a period that covers multiple cell cycles.

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The contribution of glucose and glutamine to energy metabolism in newborn pig enterocytes

Cited by 16 publications

References 29 publications

Fuel Choices by Human Platelets in Human Plasma

Fuel Choices by Human Platelets in Human Plasma

Effect of dietary glutamine, glucose and/or sodium butyrate on piglet growth, intestinal environment, subsequent fattener performance, and meat quality

Method for measuring a comprehensive energy budget in a proliferating cell system over multiple cell cycles

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