The contribution of hydrogen peroxide to the radiosensitizing effect of gold nanoparticles

Khalil, Talat Tariq; Bazzi, Rana; Roux, Stéphane; Fromm, M.

doi:10.1016/j.colsurfb.2018.12.041

Cited by 15 publications

(18 citation statements)

References 55 publications

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“…H 2 O 2 , used in medicine because of its antiseptic properties (against bacteria, yeasts, fungi and viruses) [ 3 ] and promoting wound healing [ 2 ], has numerous therapeutic advantages in the treatment of certain skin diseases, such as acne [ 45 ], skin infections [ 46 ], actinic keratosis [ 47 ], seborrheic keratosis [ 48 ], squamous cell carcinoma [ 4 ]. Moreover, as a second messenger H 2 O 2 , used alone or with other compounds or nanoparticles, can also increase the sensitization of abnormal cells (such as keratinocytes in seborrheic keratosis) and ultimately the effectiveness of photodynamic therapy [ [49] , [50] , [51] ]. On the other hand, as it is pointed out, H 2 O 2 can also generate serious side effects.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

2021

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Hydrogen peroxide (H 2 O 2 ) is widely used in clinical practice due to its antiseptic properties and its ability to heal wounds. However, due to its involvement in the formation of ROS, H 2 O 2 causes several side effects, including disorders of the metabolism of skin cells and the development of chronic inflammation mediated by oxidative stress. Therefore, this study evaluated the effects of cannabidiol (CBD), a phytocannabinoid known for its antioxidant and anti-inflammatory properties, on the proteome of keratinocyte membranes exposed to H 2 O 2 . Overall, the hydrogen peroxide caused the levels of several proteins to increase, while the treatment with CBD prevented these changes. Analysis of the protein-protein interaction network showed that the significant changes mainly involved proteins with important roles in the proteasomal activity, protein folding processes (regulatory subunit of the proteasome 26S 6A, beta proteasome subunit type 1, chaperonin 60 kDa), protein biosynthesis (40S ribosomal proteins S16, S2 and ubiquitin-S27a), regulation of the redox balance (carbonyl reductase [NADPH] 1 and NAD(P)H [quinone] 1 dehydrogenase) and cell survival (14-3-3 theta protein). Additionally, CBD reduced the total amount of MDA, 4-HNE and 4-ONE-protein adducts. Therefore, we conclude that CBD partially prevents the changes induced by hydrogen peroxide by reducing oxidative stress and maintaining proteostasis networks. Moreover, our results indicate that combination therapy with CBD may bring a promising approach in the clinical use of hydrogen peroxide by preventing its pro-oxidative and pro-inflammatory effect through potential participation of CBD in membrane mediated molecular signaling.

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Section: Discussionmentioning

confidence: 99%

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

2021

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“…Some studies have utilized dimethyl sulfoxide (DMSO) as a radical scavenger in the presence of gold nanoparticles and radiation [97]. Jeynes et al showed an increased cell survival, determined by clonogenic assay, with samples treated with gold nanoparticles, irradiation and DMSO than the nanoparticles and irradiation alone [3].…”

Section: Fluorescent Dyesmentioning

confidence: 99%

Chemical Mechanisms of Nanoparticle Radiosensitization and Radioprotection: A Review of Structure-Function Relationships Influencing Reactive Oxygen Species

Howard

Sebastian

et al. 2020

IJMS

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Metal nanoparticles are of increasing interest with respect to radiosensitization. The physical mechanisms of dose enhancement from X-rays interacting with nanoparticles has been well described theoretically, however have been insufficient in adequately explaining radiobiological response. Further confounding experimental observations is examples of radioprotection. Consequently, other mechanisms have gained increasing attention, especially via enhanced production of reactive oxygen species (ROS) leading to chemical-based mechanisms. Despite the large number of variables differing between published studies, a consensus identifies ROS-related mechanisms as being of significant importance. Understanding the structure-function relationship in enhancing ROS generation will guide optimization of metal nanoparticle radiosensitisers with respect to maximizing oxidative damage to cancer cells. This review highlights the physico-chemical mechanisms involved in enhancing ROS, commonly used assays and experimental considerations, variables involved in enhancing ROS generation and damage to cells and identifies current gaps in the literature that deserve attention. ROS generation and the radiobiological effects are shown to be highly complex with respect to nanoparticle physico-chemical properties and their fate within cells. There are a number of potential biological targets impacted by enhancing, or scavenging, ROS which add significant complexity to directly linking specific nanoparticle properties to a macroscale radiobiological result.

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“…This is radically different from the in vitro result. Possible explanations could be 3-dimensional contact inhibition, slower growth rate in vivo (reducing radiosensitivity), tumor microenvironment, and the presence of free radical scavengers in vivo 33 . Even at 20 Gy there was no significant tumor cell killing from the radiation only (Fig.…”

Section: Discussionmentioning

confidence: 99%

Iodine nanoparticles enhance radiotherapy of intracerebral human glioma in mice and increase efficacy of chemotherapy

Hainfeld

Ridwan

Stanishevskiy

et al. 2019

Sci Rep

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Gliomas and other brain tumors have evaded durable therapies, ultimately causing about 20% of all cancer deaths. Tumors are widespread in the brain at time of diagnosis, limiting surgery and radiotherapy effectiveness. Drugs are also poorly effective. Radiotherapy (RT) is limited by dose to normal tissue. However, high-atomic-number elements absorb X-rays and deposit the absorbed dose locally, even doubling (or more) the local dose. Previously we showed that gold nanoparticles (AuNPs) with RT could eradicate some brain tumors in mice and many other preclinical studies confirmed AuNPs as outstanding radioenhancers. However, impediments to clinical translation of AuNPs have been poor clearance, skin discoloration, and cost. We therefore developed iodine nanoparticles (INPs) that are almost colorless, non-toxic, lower cost, and have reasonable clearance, thus overcoming major drawbacks of AuNPs. Here we report the use of iodine nanoparticle radiotherapy (INRT) in treating advanced human gliomas (U87) grown orthotopically in nude mice resulting in a more than a doubling of median life extension compared to RT alone. Significantly, INRT also enhanced the efficacy of chemotherapy when it was combined with the chemotherapeutic agent Doxil, resulting in some longer-term survivors. While ongoing optimization studies should further improve INRT, clinical translation appears promising.

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The contribution of hydrogen peroxide to the radiosensitizing effect of gold nanoparticles

Cited by 15 publications

References 55 publications

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

Protective effects of cannabidiol on the membrane proteins of skin keratinocytes exposed to hydrogen peroxide via participation in the proteostasis network

Chemical Mechanisms of Nanoparticle Radiosensitization and Radioprotection: A Review of Structure-Function Relationships Influencing Reactive Oxygen Species

Iodine nanoparticles enhance radiotherapy of intracerebral human glioma in mice and increase efficacy of chemotherapy

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