The contribution of in vitro technology and cryogenic storage to conservation of indigenous plants

Bunn, Eric; Turner, Shane R.; Panaia, M.; Dixon, Kingsley W.

doi:10.1071/bt06065

Cited by 50 publications

(41 citation statements)

References 28 publications

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“…× E. rudis Endl. [162], 0.25, 2.5, or 5 µM IBA with 0.25 or 0.5 µM NAA for E. impensa [70], and 2.5 µM IBA with 2.5 µM NAA for E. sideroxylon [85]. NAA has been used alone to induce roots at 10.8 µM for C. citriodora [69], 2.7 µM for E. grandis and E. grandis × E. urophylla [124], 1.1 or 2.7 µM for E. pellita [100], and 12.5 µM for E. urophylla × E. grandis [163].…”

Section: Adventitious Root Formationmentioning

confidence: 99%

“…However, adventitious rooting on eucalypt shoots is usually induced with an auxin rooting hormone, typically IBA at a concentration between 0.49 and 49 µM (Table A1). IBA has also been used in combination with NAA to induce adventitious eucalypt roots, including 5 µM IBA with 0.5 or 1.0 µM NAA for E. dolorosa Brooker & Hopper [162], 0.25 µM IBA with 0.25 µM NAA, or 0.5 µM IBA with 0.5 µM NAA, for E. drummondii Benth. × E. rudis Endl.…”

Section: Adventitious Root Formationmentioning

confidence: 99%

See 1 more Smart Citation

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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Section: Adventitious Root Formationmentioning

confidence: 99%

Section: Adventitious Root Formationmentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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“…However, several in vitro and tissue culture techniques have been known for its conservation properties by maintaining the genetic homogeneity of the regenerated plantlets from the stored germplasms (Engelmann 2012) as these techniques confirmed the mid-term storage of plant materials ensuring higher germination rates (Bunn et al 2007). But on the other side for long-term storage of plant germplasms, cryopreservation is the lone technique.…”

Section: Forest Seeds: Concernsmentioning

confidence: 99%

Cryopreservation of Forest Tree Seeds: A Mini-Review

Gantait¹,

Kundu²,

Wani³

et al. 2016

Journal of Forest and Environmental Science

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Since forest trees form the basis of forest ecosystem, their prolong subsistence is crucial for various flora and fauna. The foremost challenges to sustain the forest ecosystem comprise of the declining forest tree population accompanied with structural changes due to afforestation and exploitation of forest area, environment changes, pests, pollution, and introgressive hybridization. For ex situ conservation approach, in vitro techniques encompass basic role for conserving tree genetic resources, predominantly where natural propagules like recalcitrant seed might not be appropriate for long-term conservation. The practice includes restricted growth techniques, conventional micropropagation, production and storage of synthetic seeds, and cryopreservation. Even though these practices have been applied chiefly to herbaceous species, but recently, woody species were also focused upon. Key conceptions, challenges and techniques for forest tree seed conservation are discussed briefly in this review with special emphasis on some successful cryopreservation approaches for long-term storage.

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“…Tissue culture techniques are of great interest for collecting, multiplication and storage of plant germplasm and are very useful for conserving plant biodiversity, including (a) genetic resources of recalcitrant seed and vegetatively propagated species; (b) rare and endangered plant species; and (c) biotechnology products, such as elite genotypes and genetically engineered material [6,31,32]. Tissue culture systems allow propagating plant material with high multiplication rates in an aseptic environment.…”

Section: In Vitro Technologies For Propagation and Exchange Of Plant mentioning

confidence: 99%

“…There are several examples to illustrate the application of cryopreservation techniques above described to preserve rare and endangered species for various higher plants [32], including orchids [118], bryophytes and ferns [119].…”

Section: Long-term Conservation Through Cryopreservationmentioning

confidence: 99%

Biotechnology and Conservation of Plant Biodiversity

2013

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Advances in plant biotechnology provide new options for collection, multiplication and short-to long-term conservation of plant biodiversity, using in vitro culture techniques. Significant progress has been made for conserving endangered, rare, crop ornamental, medicinal and forest species, especially for non-orthodox seed and vegetatively propagated plants of temperate and tropical origin. Cell and tissue culture techniques ensure the rapid multiplication and production of plant material under aseptic conditions. Medium-term conservation by means of in vitro slow growth storage allows extending subcultures from several months to several years, depending on the species. Cryopreservation (liquid nitrogen, −196 °C) is the only technique ensuring the safe and cost-effective long-term conservation of a wide range of plant species. Cryopreservation of shoot tips is also being applied to eradicate systemic plant pathogens, a process termed cryotherapy. Slow growth storage is routinely used in many laboratories for medium-conservation of numerous plant species. Today, the large-scale, routine application of cryopreservation is still restricted to a limited number of cases. However, the number of plant species for which cryopreservation techniques are established and validated on a large range of genetically diverse accessions is increasing steadily.

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The contribution of in vitro technology and cryogenic storage to conservation of indigenous plants

Cited by 50 publications

References 28 publications

Tissue Culture of Corymbia and Eucalyptus

Tissue Culture of Corymbia and Eucalyptus

Cryopreservation of Forest Tree Seeds: A Mini-Review

Biotechnology and Conservation of Plant Biodiversity

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