The contribution of PFAS to thyroid hormone-displacing activity in Dutch waters: A comparison between two in vitro bioassays with chemical analysis

de Schepper, J.K.H.; van Oorschot, Y.; Jaspers, R.J.; Hamers, T.; Lamoree, M.H.; Behnisch, P.; Besselink, H.; Houtman, C.J.

doi:10.1016/j.envint.2023.108256

Cited by 6 publications

(8 citation statements)

References 32 publications

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“…Persistent, so-called environmentally toxic chemicals (e.g., triclosan and DDAC) enter waterways and the atmosphere, may enrich in food chains, and, in the long term, harm the reproduction and health of humans and other organisms [ 1 , 30 , 84 , 85 ]. The time of exposure to these substances during fetal development can determine the appearance of symptoms and level of harm to health [ 36 , 86 , 87 ].…”

Section: Discussionmentioning

confidence: 99%

“…Ex Vivo BSMI In Vitro ICP Mitochondrial Toxicity 1 Wet Buildings Containing Visible Mold Growth (n = 12) 1 Measured as accelerated glycolysis and/or as a decrease in mitochondrial membrane potential ∆Ψm < 100 mV. 2 No health complaints reported.…”

Section: Toxicitymentioning

confidence: 99%

“…Bioassays do not reveal the identities of individual compounds or their origin. Testing the biological activity of chromatographic fractions and chemical identification of the active fractions are essential for effect-directed analysis (EDA) [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

“…Exposure to toxic molds or their toxins is expected to cause more- or less-acute symptoms. Meanwhile, exposure during pregnancy to hormone disruptors and reproductive toxins such as phthalates, triclosan, and per- and polyfluoroalkyl substances may affect the offspring in the next generation [ 1 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

“…By EDA analysis, various toxic compounds have been detected, isolated, purified, and identified by the toxicological assessments of drinking water, indoor dusts, building materials, foods, and indoor microbial cultures [1][2][3][4][5][6][7]. Bioassays have been used to reveal and separate several specific toxicity mechanisms as hormone-disrupting activity and mitochondrial toxicity of xenobiotic contaminants.…”

Section: Introductionmentioning

confidence: 99%

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Toxicity Screening of Fungal Extracts and Metabolites, Xenobiotic Chemicals, and Indoor Dusts with In Vitro and Ex Vivo Bioassay Methods

Hintikka,

Andersson,

Lundell

et al. 2024

Pathogens

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It is controversial how useful bioassays are for identifying the in vivo toxicity of hazardous environmental exposures. In this study, fruiting bodies of forest mushrooms (n = 46), indoor mold colonies (n = 412), fungal secondary metabolites (n = 18), xenobiotic chemicals such as biocides and detergents (n = 6), and methanol extracts of indoor dusts from urban buildings (n = 26) were screened with two different bioactivity assays: boar sperm motility inhibition (BSMI) and inhibition of cell proliferation (ICP) tests. For the forest mushrooms, the toxicity testing result was positive for 100% of poisonous-classified species, 69% of non-edible-classified species, and 18% of edible-classified species. Colonies of 21 isolates of Ascomycota mold fungal species previously isolated from water-damaged buildings proved to be toxic in the tests. Out of the fungal metabolites and xenobiotic chemicals, 94% and 100% were toxic, respectively. Out of the indoor dusts from moldy-classified houses (n = 12) and from dry, mold-free houses (n = 14), 50% and 57% were toxic, respectively. The bioassay tests, however, could not differentiate the samples from indoor dusts of moldy-classified buildings from those from the mold-free buildings. Xenobiotic chemicals and indoor dusts were more toxic in the BSMI assay than in the ICP assay, whereas the opposite results were obtained with the Ascomycota mold colonies and fungal secondary metabolites. The tests recognized unknown methanol-soluble thermoresistant substances in indoor settled dusts. Toxic indoor dusts may indicate a harmful exposure, regardless of whether the toxicity is due to xenobiotic chemicals or microbial metabolites.

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