The Contribution of the Bacterial Components of Adjuvant in the Induction of Aspermatogenesis and in the Sensitization of the Ilea of Guinea Pigs

Katsh, Seymour

doi:10.1159/000229042

Cited by 18 publications

(11 citation statements)

References 5 publications

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“…Certain other organisms such as Nocardia asteroides (7), and C. rubrum (8) as well as certain principles extracted from Mycobacteria (4, 7) and C. rubrum ( K a tsh a n d Crowle, to be published) can replace the whole cells. The activity of the factors extracted from M. tuberculosis appears to be, in part, related to a glycolipid or lipolysaccharide (4,7). It is interesting, therefore, that the lipolysaccharides of typhimurium, typhosa or coli (table II) are, by comparison, inefficient in sponsoring testicular damage (4).…”

Section: Discussionmentioning

confidence: 99%

“…The activity of the factors extracted from M. tuberculosis appears to be, in part, related to a glycolipid or lipolysaccharide (4,7). It is interesting, therefore, that the lipolysaccharides of typhimurium, typhosa or coli (table II) are, by comparison, inefficient in sponsoring testicular damage (4). The fact that sulfolipids from tubercle bacilli were found to be de void of activity of this nature may indicate an important contribu tion of the carbohydrate moiety.…”

Section: Discussionmentioning

confidence: 99%

“…This was observed in ancillary experiments by dissolving Sudan Black B and Sudan III (oil-soluble dyes) in adjuvant and color contrasting dyes (thiazine red, eosin, fast green or brilliant cresyl blue) in saline-antigen mixture. In any event the importance of lymphoid organ response is, reemphasized (4,9) in the following way. If antigen and bacterial factor can be released simultaneously or within a reasonably close time limit, effective transport to drain ing lymph nodes can be accomplished.…”

Section: Discussionmentioning

confidence: 99%

“…In this endeavor, attention was focused primarily upon oils of vegetable origin. The general pro cedure for preparing the respective adjuvant-antigen mixture and the injection routine have been described in detail elsewhere (4). Briefly, the oil under investigation was admixed thoroughly with the respective emulsifier (table I) in a glass vial.…”

Section: Materials and Methods Comparisons Of Ability Of Adjuvants Tomentioning

confidence: 99%

“…The animals were sacrificed 2 months following injection and the organs were treated as described in detail elsewhere (4). The testicular damage rating scale applied to histological observations with ascending numerals indicating increased depiction of semi niferous epithelium (5).…”

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confidence: 99%

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Adjuvants and Aspermatogenesis in the Guinea Pig

Katsh¹

1964

Int Arch Allergy Immunol

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Materials and Methods Comparisons Of Ability Of Adjuvants Tomentioning

confidence: 99%

mentioning

confidence: 99%

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Adjuvants and Aspermatogenesis in the Guinea Pig

Katsh¹

1964

Int Arch Allergy Immunol

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Effects of immunization with Freund's complete adjuvant and isologous spermatozoa on the seminiferous epithelium and blood‐testis barrier in guinea pigs

et al. 1981

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In order to gain insight into the earliest pathological changes underlying the development of autoimmune aspermatogenic orchitis (AIAO) the blood-testis barrier was studied by light and electron microscopy, freeze-etching, and cytochemical techniques early (from 1 to 8 days after adjuvant treatment of isoimmunization). At later times (16 to 21 days) the study was carried out by light microscopy only. Adult male guinea pigs were used either as controls or immunized with Freund's complete adjuvant alone or together with pertussis vaccine. An additional group comprised animals immunized with a suspension of isologous spermatozoa emulsified in Freund's complete adjuvant and with pertussis vaccine. Ultrastructural studies of the testes of experimental animals showed, at earlier periods, apparently normal Sertoli junctions. However, in the adluminal compartment, distended gaps were seen between the facing membranes of adjacent Sertoli cells. At later periods, a massive destruction of the germinal cells were observed. In freeze-fracture replicas, the Sertoli junctions of testes belonging to all the experimental groups were characterized by an irregular network of occasionally interrupted strands of particles associated with the P face (PF). Large concavities determined distensions between interconnecting ridges. The gap junctions were increased in number and in surface. Tracer studies using horseradish peroxidase showed that the marker permeated the myoid cells of a greater proportion of tubules than in control animals. Within the seminiferous epithelium there was only a limited passage of the marker towards the lumina of the tubules. Yet the tracer was always excluded from the adluminal compartment by the Sertoli tight junctions. Our observations suggest the possibility that the FCA causes a loosening of the Sertoli junctions. This condition could enhance exchanges between two antigenically different cellular compartments and, thus, favor occurrence of an autoimmune reaction when cytotoxic factors are experimentally induced, as in iso- or autoimmunization.

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The Reproductive System

Shulman¹

1974

Molecular Biology Biochemistry and Biophysics

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The Contribution of the Bacterial Components of Adjuvant in the Induction of Aspermatogenesis and in the Sensitization of the Ilea of Guinea Pigs

Cited by 18 publications

References 5 publications

Adjuvants and Aspermatogenesis in the Guinea Pig

Adjuvants and Aspermatogenesis in the Guinea Pig

Effects of immunization with Freund's complete adjuvant and isologous spermatozoa on the seminiferous epithelium and blood‐testis barrier in guinea pigs

The Reproductive System

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