The Contribution of Various In Vitro Methodologies to Comprehending the Filling Ability of Root Canal Pastes in Primary Teeth

Abstract

A void-free obturation during root canal treatment on primary teeth is currently very difficult to attain. In this study, the pulpectomy filling abilities of Bio-C Pulpecto (Angelus, Basil, Londrina, Paraná, Brazil) and of zinc oxide eugenol, or “ZOE” (DenPro, Prevest, New York, NY, USA), were compared using several in vitro techniques. Therefore, 30 primary anterior teeth were used in the present in vitro study. Analysis of variance (ANOVA), including a multiple comparison procedure (Holm-Sidak method, Dunn’s Method, or Tukey test), was used. On micro-CT, Bio-C Pulpecto exhibited higher void percentages than did ZOE (10.3 ± 3.8%, and 3.5 ± 1.3%), respectively (p < 0.05). With digital microscopy, higher total void percentages were found in the BC (13.2 ± 26.7%) group compared to the ZOE (2.7 ± 2.8%) group (p < 0.05). With the CLSM, mean tubular penetration depths were higher for Bio-C Pulpecto than for ZOE in all canal thirds (p < 0.05). SEM images demonstrated no tags into dentinal tubules in either group throughout the three thirds. Moreover, higher statistically significant flowability was found for Bio-C (2.657 ± 0.06 mm) compared to ZOE (1.8 ± 0.13 mm) (p < 0.05). The findings of this study indicate that neither ZOE nor Bio-C Pulpecto appears to meet the criteria for an ideal root canal filling paste for primary teeth. This study laid the groundwork for future research by determining how micro-CT, digital microscopy, SEM, and CLSM contribute to our understanding of the filling process of primary teeth. More thorough research on the mechanism of root canal obturation on primary teeth is required to achieve a long-term successful root canal therapy in young children.

Evaluation of Solubility, and Volumetric and Morphological Alterations of Bioceramic Filling Material for Primary Teeth: A New Methodological Approach

“…The filling capacity of all materials was higher than 98%, regardless of the obturation technique applied. In the present study, the filling capacity was assessed by micro-CT; however, other methodologies such as digital microscopy, confocal laser scanning microscopy analysis (CLSM), or even SEM could also be used, as previously described [ 32 ]. It is noteworthy that the phase separation of Bio-CP caused by the chemical instability of the material did not influence the filling capacity percentage.…”

Comparative Evaluation of the Quality of Obturation Between Three Obturation Techniques in Primary Canines: An In Vitro Study

“…It is challenging to obtain a void-free root canal filling in clinical pulpectomy practice today, and the success of pulpectomy in primary teeth depends on obturation quality. Therefore, there is no consensus in the literature about the approach offering the best seal for the root canal system in primary teeth [ 12 - 14 ]. This study aimed to compare the quality of three obturation techniques (rotary lentulo spiral, handheld lentulo spiral, and pressure syringe) for two fillings (ZOE paste and Metapex) in primary canines using digital radiography to evaluate the quality of obturation.…”