The control of alginate degradation to dynamically manipulate scaffold composition for in situ transfection application

Hu, Wei; Hu, Zhe Chen

doi:10.1016/j.ijbiomac.2018.06.016

Cited by 14 publications

(6 citation statements)

References 30 publications

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“…Alginate adsorbed the cationic polyethylenimine/DNA complexes, with higher alginate content resulting in increased immobilization of non-viral vectors. By optimizing the ratio of PCL and alginate, the in situ transfection study verified that the composite nanofibers delivered target genes to the adhered cells and improved cell viability ( Figure 3 C) [ 70 , 71 ]. Wen et al (2018) prepared a core–sheath nanofibrous colon-specific delivery system of quercetin and prebiotics with enhanced anticancer properties.…”

Section: Seaweed-derived Biopolymersmentioning

confidence: 99%

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

et al. 2022

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Marine biopolymers, abundantly present in seaweeds and marine animals, feature diverse structures and functionalities, and possess a wide range of beneficial biological activities. Characterized by high biocompatibility and biodegradability, as well as unique physicochemical properties, marine biopolymers are attracting a constantly increasing interest for the development of advanced systems for applications in the biomedical field. The development of electrospinning offers an innovative technological platform for the production of nonwoven nanofibrous scaffolds with increased surface area, high encapsulation efficacy, intrinsic interconnectivity, and structural analogy to the natural extracellular matrix. Marine biopolymer-based electrospun nanofibrous scaffolds with multifunctional characteristics and tunable mechanical properties now attract significant attention for biomedical applications, such as tissue engineering, drug delivery, and wound healing. The present review, covering the literature up to the end of 2021, highlights the advancements in the development of marine biopolymer-based electrospun nanofibers for their utilization as cell proliferation scaffolds, bioadhesives, release modifiers, and wound dressings.

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Section: Seaweed-derived Biopolymersmentioning

confidence: 99%

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

et al. 2022

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“…Although the method does not impact the therapeutic activity of loaded cargoes and numerous studies have employed this approach in the design of optimal electrospun fibrous architectures for gene therapy drug delivery, the uncontrolled release profile still remains a critical disadvantage [5]. According to the literature, for the first time, Hu and co-workers [95,96] addressed the electrostatic interactions to immobilize PEI/DNA complexes onto nanofibrous scaffolds for in situ transfection applications. In this context, using a dual-jet system, the authors combined the contributions of two different polymers, the natural polycationic alginate and the synthetic PCL, into electrospun fibrous architectures, which, on one side, assures the necessary negative functionalities for the immobilization of positively charged PEI/DNA polyplexes and, on the other site, improves the biocompatibility and transfection rate.…”

Section: Plasma Treatmentmentioning

confidence: 99%

“…Considering that this design principle allows high versatility in the construction of fibrous architectures with physico-chemical, morphological and biological features that can be easily modulated to better suit the mechanical, biological and physical demand of the targeted host tissue, this thriving approach is of particular importance to regenerative medicine. Therefore, numerous blends consisting of synthetic and natural polymers in different formulations (e.g., PCL/alginate [96], PLLA/Collagen [118], PCL/silk fibroin [120], Gelatin/Collagen/PEG [144], PLLA/silk fibroin [145]) were constructed and investigated as electrospun fibrous strategies for the efficient delivery of genetic material. For instance, Zhao et al [118] managed to substantially improve transfection efficiency and rhBMP-2 transgene mRNA expression as well as to induce ectopic bone formation both in vitro and in vivo by adsorbing rhBMP-2/p-DNA complexes onto PLLA/Collagen I electrospun scaffolds.…”

Section: Natural/synthetic Polymer-based Electrospun-fibrous-architec...mentioning

confidence: 99%

Electrospun-Fibrous-Architecture-Mediated Non-Viral Gene Therapy Drug Delivery in Regenerative Medicine

2022

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Gene-based therapy represents the latest advancement in medical biotechnology. The principle behind this innovative approach is to introduce genetic material into specific cells and tissues to stimulate or inhibit key signaling pathways. Although enormous progress has been achieved in the field of gene-based therapy, challenges connected to some physiological impediments (e.g., low stability or the inability to pass the cell membrane and to transport to the desired intracellular compartments) still obstruct the exploitation of its full potential in clinical practices. The integration of gene delivery technologies with electrospun fibrous architectures represents a potent strategy that may tackle the problems of stability and local gene delivery, being capable to promote a controlled and proficient release and expression of therapeutic genes in the targeted cells, improving the therapeutic outcomes. This review aims to outline the impact of electrospun-fibrous-architecture-mediated gene therapy drug delivery, and it emphatically discusses the latest advancements in their formulation and the therapeutic outcomes of these systems in different fields of regenerative medicine, along with the main challenges faced towards the translation of promising academic results into tangible products with clinical application.

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“…Other polymers were also used as carrier polymers for facilitating the spinnability of alginate [57,58]. Polylactic acid (PLA) as the continuous phase and sodium alginate as the dispersion phase were used by Xu et al [59] to prepare a W/O emulsion and then electrospun into nanofibrous membrane for tissue engineering.…”

Section: Other Polymersmentioning

confidence: 99%

Electrospun Alginate Nanofibers Toward Various Applications: A Review

et al. 2020

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Alginate has been a material of choice for a spectrum of applications, ranging from metal adsorption to wound dressing. Electrospinning has added a new dimension to polymeric materials, including alginate, which can be processed to their nanosize levels in order to afford unique nanostructured materials with fascinating properties. The resulting nanostructured materials often feature high porosity, stability, permeability, and a large surface-to-volume ratio. In the present review, recent trends on electrospun alginate nanofibers from over the past 10 years toward advanced applications are discussed. The application of electrospun alginate nanofibers in various fields such as bioremediation, scaffolds for skin tissue engineering, drug delivery, and sensors are also elucidated.

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The control of alginate degradation to dynamically manipulate scaffold composition for in situ transfection application

Cited by 14 publications

References 30 publications

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

Electrospun-Fibrous-Architecture-Mediated Non-Viral Gene Therapy Drug Delivery in Regenerative Medicine

Electrospun Alginate Nanofibers Toward Various Applications: A Review

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