The Control of Calcium Metabolism in Zebrafish (Danio rerio)

Lin, Cheng Huang; Hwang, Pung‐Pung

doi:10.3390/ijms17111783

Cited by 59 publications

(30 citation statements)

References 116 publications

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“…Zebrafish are exposed to various Ca 2+ concentrations in their natural environment, from 0.01 mM (soft water) to 3 mM (hard water) ( Hoenderop et al, 2005 ; Pan et al, 2005 ; Lin and Hwang, 2016 ; USGS Office of Water Quality, 2017 ). Studies of fish have indicated strong CaSR expression in gills and the olfactory epithelium ( Loretz et al, 2004 ; Loretz, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Role of Calcium-Sensing Receptor in Mechanotransducer-Channel-Mediated Ca2+ Influx in Hair Cells of Zebrafish Larvae

Yeh

Hung

et al. 2018

Front. Physiol.

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The calcium-sensing receptor (CaSR) is an extracellular Ca2+ sensor that plays a critical role in maintaining Ca2+ homeostasis in several organs, including the parathyroid gland and kidneys. In this study, through in situ hybridization, the expression of CaSR mRNA was found in the neuromasts of zebrafish larvae. Immunohistochemistry further demonstrated that the CaSR protein was present in neuromast hair cell stereocilia and basolateral membranes. Based on the expression and subcellular localization of the CaSR in hair cells, we hypothesized that the CaSR is expressed in zebrafish lateral-line hair cells to regulate mechanotransducer (MET)-channel-mediated Ca2+ entry. Using the scanning ion-selective electrode technique, MET-channel-mediated Ca2+ influx at the stereocilia of hair cells was measured in intact larvae. Ca2+ influx was suppressed after larvae were pretreated with a CaSR activator (R-568) or high-Ca2+ (HCa) medium. Gene knockdown by using morpholino oligonucleotides decreased CaSR expression in hair cells and eliminated the effects of R-568 and HCa on Ca2+ influx. In addition, we found that treatment with R-568 attenuated neomycin-induced hair cell death. This study is the first to demonstrate that the CaSR is involved in mechanotransduction in zebrafish hair cells.

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Section: Discussionmentioning

confidence: 99%

Role of Calcium-Sensing Receptor in Mechanotransducer-Channel-Mediated Ca2+ Influx in Hair Cells of Zebrafish Larvae

Yeh

Hung

et al. 2018

Front. Physiol.

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“…Many hormones known to be critical to mammalian bone mineralization, including Vitamin D3, 304 parathyroid hormone, calcitonin were found to be important in zebrafish (Lin & Hwang, 2016). Using an optimized in situ hybridization protocol and qPCR analysis of FACS isolated 315 NaR cells, we found that Papp-aa is highly expressed in NaR cells.…”

Section: Nar Cells 243mentioning

confidence: 93%

The metalloproteinase Papp-aa controls epithelial cell quiescence-proliferation transition

Liu

Noer

et al. 2020

eLife

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Human patients carrying PAPP‐A2 inactivating mutations have low bone mineral density. The underlying mechanisms for this reduced calcification are poorly understood. Using a zebrafish model, we report that Papp-aa regulates bone calcification by promoting Ca2+-transporting epithelial cell (ionocyte) quiescence-proliferation transition. Ionocytes, which are normally quiescent, re-enter the cell cycle under low [Ca2+] stress. Genetic deletion of Papp-aa, but not the closely related Papp-ab, abolished ionocyte proliferation and reduced calcified bone mass. Loss of Papp-aa expression or activity resulted in diminished IGF1 receptor-Akt-Tor signaling in ionocytes. Under low Ca2+ stress, Papp-aa cleaved Igfbp5a. Under normal conditions, however, Papp-aa proteinase activity was suppressed and IGFs were sequestered in the IGF/Igfbp complex. Pharmacological disruption of the IGF/Igfbp complex or adding free IGF1 activated IGF signaling and promoted ionocyte proliferation. These findings suggest that Papp-aa-mediated local Igfbp5a cleavage functions as a [Ca2+]-regulated molecular switch linking IGF signaling to bone calcification by stimulating epithelial cell quiescence-proliferation transition under low Ca2+ stress.

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“…The processes in the uptake and acid-base regulation in zebrafish have been comprehensively discussed in several previous reviews [ 1 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. However, no article has systematically reviewed the similarities and differences in the regulation of epithelial ion transporters between zebrafish and mammals during metabolic acidosis.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish as a Model System for Investigating the Compensatory Regulation of Ionic Balance during Metabolic Acidosis

Lewis

Kwong

2018

IJMS

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Zebrafish (Danio rerio) have become an important model for integrative physiological research. Zebrafish inhabit a hypo-osmotic environment; to maintain ionic and acid-base homeostasis, they must actively take up ions and secrete acid to the water. The gills in the adult and the skin at larval stage are the primary sites of ionic regulation in zebrafish. The uptake of ions in zebrafish is mediated by specific ion transporting cells termed ionocytes. Similarly, in mammals, ion reabsorption and acid excretion occur in specific cell types in the terminal region of the renal tubules (distal convoluted tubule and collecting duct). Previous studies have suggested that functional regulation of several ion transporters/channels in the zebrafish ionocytes resembles that in the mammalian renal cells. Additionally, several mechanisms involved in regulating the epithelial ion transport during metabolic acidosis are found to be similar between zebrafish and mammals. In this article, we systemically review the similarities and differences in ionic regulation between zebrafish and mammals during metabolic acidosis. We summarize the available information on the regulation of epithelial ion transporters during acidosis, with a focus on epithelial Na+, Cl− and Ca2+ transporters in zebrafish ionocytes and mammalian renal cells. We also discuss the neuroendocrine responses to acid exposure, and their potential role in ionic compensation. Finally, we identify several knowledge gaps that would benefit from further study.

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The Control of Calcium Metabolism in Zebrafish (Danio rerio)

Cited by 59 publications

References 116 publications

Role of Calcium-Sensing Receptor in Mechanotransducer-Channel-Mediated Ca2+ Influx in Hair Cells of Zebrafish Larvae

Role of Calcium-Sensing Receptor in Mechanotransducer-Channel-Mediated Ca2+ Influx in Hair Cells of Zebrafish Larvae

The metalloproteinase Papp-aa controls epithelial cell quiescence-proliferation transition

Zebrafish as a Model System for Investigating the Compensatory Regulation of Ionic Balance during Metabolic Acidosis

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