The control of smooth muscle tissues by nonadrenergic noncholinergic(NANC) nerve fibres in the autonomic nervous system.

Itoh, Hiroyuki; Sakai, J; Imoto, Akihiko; Creed, Kate E.

doi:10.1540/jsmr.31.67

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…Moreover VIP has already been found in association with DßH in some vasodilatory neurons of porcine thoracic sympathetic chain ganglia. 26,48 However, the coexistence of TH and VIP is unusual for a peptide commonly thought to be a marker of the cholinergic pathway or a classical neurotransmitter of the inhibitory non-adrenergic-non-cholinergic (NANC) subdivision of the autonomic nervous system; 89,90 in addition, according to Hill and Elde 48 these TH/VIP-IR neurons could have a vasodilatory action as well.…”

Section: Discussionmentioning

confidence: 99%

Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

et al. 2013

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The urinary bladder trigone (UBT) is a limited area through which the majority of vessels and nerve fibers penetrate into the urinary bladder and where nerve fibers and intramural neurons are more concentrated. We localized the extramural post-ganglionic autonomic neurons supplying the porcine UBT by means of retrograde tracing (Fast Blue, FB). Moreover, we investigated the phenotype of sympathetic trunk ganglia (STG) and caudal mesenteric ganglia (CMG) neurons positive to FB (FB+) by coupling retrograde tracing and double-labeling immunofluorescence methods. A mean number of 1845.1±259.3 FB+ neurons were localized bilaterally in the L1-S3 STG, which appeared as small pericarya (465.6±82.7 µm2) mainly localized along an edge of the ganglion. A large number (4287.5±1450.6) of small (476.1±103.9 µm2) FB+ neurons were localized mainly along a border of both CMG. The largest number (4793.3±1990.8) of FB+ neurons was observed in the pelvic plexus (PP), where labeled neurons were often clustered within different microganglia and had smaller soma cross-sectional area (374.9±85.4 µm2). STG and CMG FB+ neurons were immunoreactive (IR) for tyrosine hydroxylase (TH) (66±10.1% and 52.7±8.2%, respectively), dopamine beta-hydroxylase (DβH) (62±6.2% and 52±6.2%, respectively), neuropeptide Y (NPY) (59±8.2% and 65.8±7.3%, respectively), calcitonin-gene-related peptide (CGRP) (24.1±3.3% and 22.1±3.3%, respectively), substance P (SP) (21.6±2.4% and 37.7±7.5%, respectively), vasoactive intestinal polypeptide (VIP) (18.9±2.3% and 35.4±4.4%, respectively), neuronal nitric oxide synthase (nNOS) (15.3±2% and 32.9±7.7%, respectively), vesicular acetylcholine transporter (VAChT) (15±2% and 34.7±4.5%, respectively), leuenkephalin (LENK) (14.3±7.1% and 25.9±8.9%, respectively), and somatostatin (SOM) (12.4±3% and 31.8±7.3%, respectively). UBT-projecting neurons were also surrounded by VAChT-, CGRP-, LENK-, and nNOSIR fibers. The possible role of these neurons and fibers in the neural pathways of the UBT is discussed.

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Section: Discussionmentioning

confidence: 99%

Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

et al. 2013

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“…The normal process of micturition involves an initial urethral relaxation, followed by an increase of intravesical pressure, as well as a maintained relaxation of the urethra (Tanagho & Miller, 1970) and pharmacological evidence indicates that NO is the inhibitory NANC neurotransmitter in the urethra and bladder neck (Itoh et al , 1995). In hamster proximal urethra, nitric oxide is involved in the initial phase of the relaxation and ATP is involved in the second phase of the response (Pinna et al , 1996).…”

Section: Discussionmentioning

confidence: 99%

ATP and vasoactive intestinal polypeptide relaxant responses in hamster isolated proximal urethra

Pinna

Puglisi

Burnstock

1998

British J Pharmacology

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1 Nitric oxide (NO) is known from previous studies to be the principle transmitter in NANC inhibitory nerves supplying the hamster urethra. However, the identity of the cotransmitter(s) responsible for the responses remaining following block with L-N G -nitroarginine methyl ester (L-NAME) is not known. 6 In summary, these results are consistent with the view that ATP is an inhibitory transmitter released from inhibitory nerves supplying the NANC relaxation of hamster proximal urethra. The relaxant eect of ATP is NO-and urothelium-independent. The present study did not demonstrate whether VIP is released from parasympathetic nerves during EFS, since both a-chymotrypsin and [Lys 1 , Pro 2,5 , Arg 3,4 , Tyr 6 ]-VIP were ineective on neurogenic responses.

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“…However, VIP has already been found in association with DβH in some vasodilatory neurons of the porcine thoracic sympathetic chain ganglia (Hill and Elde, 1989;Majewski, 1999). The coexistence of TH and VIP is unusual for a peptide commonly thought to be a marker of the cholinergic pathway or a classical neurotransmitter of the inhibitory-non adrenergic/ non cholinergic (NANC) subdivision of the autonomic nervous system (Itoh et al, 1995).…”

Section: Vip-immunoreactivitymentioning

confidence: 99%

Double labelling immunohistochemical characterization of autonomic sympathetic neurons innervating the sow retractor clitoridis muscle

Ragionieri¹,

Botti²,

Gazza³

et al. 2009

Eur J Histochem

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Retrograde neuronal tracing and immunohistochemical methods were used to define the neurochemical content of sympathetic neurons projecting to the sow retractor clitoridis muscle (RCM). Differently from the other smooth muscles of genital organs, the RCM is an isolated muscle that is tonically contracted in the rest phase and relaxed in the active phase. This peculiarity makes it an interesting experimental model. The fluorescent tracer fast blue was injected into the RCM of three 50 kg subjects. After a one-week survival period, the ipsilateral paravertebral ganglion S1, that in a preliminary study showed the greatest number of cells projecting to the muscle, was collected from each animal. The co-existence of tyrosine hydroxylase with choline acetyltransferase, neuronal nitric oxide synthase, calcitonin gene-related peptide, leuenkephalin, neuropeptide Y, substance P and vasoactive intestinal polypeptide was studied under a fluorescent microscope on cryostat sections. Tyrosine hydroxylase was present in about 58% of the neurons projecting to the muscle and was found to be co-localized with each of the other tested substances. Within fast blue-labelled cells negative to the adrenergic marker, small populations of neurons singularly containing each of the other enzymatic markers or peptides were also observed. The present study documents the complexity of the neurochemical interactions that regulate the activity of the smooth myocytes of the RCM and their vascular components.

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The control of smooth muscle tissues by nonadrenergic noncholinergic(NANC) nerve fibres in the autonomic nervous system.

Cited by 10 publications

References 37 publications

Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

ATP and vasoactive intestinal polypeptide relaxant responses in hamster isolated proximal urethra

Double labelling immunohistochemical characterization of autonomic sympathetic neurons innervating the sow retractor clitoridis muscle

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