The conventionally ventilated operating theatre and air contamination control during cardiac surgery – bacteriological and particulate matter control garment options for low level contamination

Verkkala, K; Eklund, Anne; Ojajärvi, Juhani; Tiittanen, L.; Hoborn, Jan; Mäkelä, P H

doi:10.1016/s1010-7940(98)00150-x

Cited by 49 publications

(23 citation statements)

References 9 publications

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“…Some countries have set airborne bacterial limits for operating theatres [15]. Microbial air contamination of operating theatres during surgical procedures could be a risk factor for surgical site infection through settling of airborne bacteria [10,16]. The presented results show higher airborne bacterial concentrations than the limits.…”

Section: Resultsmentioning

confidence: 72%

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Monitoring of airborne bacteria and aerosols in different wards of hospitals – Particle counting usefulness in investigation of airborne bacteria

Mirhoseini¹,

Nikaeen²,

Khanahmd³

et al. 2015

Ann Agric Environ Med.

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Introduction and objective. The presence of airborne bacteria in hospital environments is of great concern because of their potential role as a source of hospital-acquired infections (HAI). The aim of this study was the determination and comparison of the concentration of airborne bacteria in different wards of four educational hospitals, and evaluation of whether particle counting could be predictive of airborne bacterial concentration in different wards of a hospital. Materials and method. The study was performed in an operating theatre (OT), intensive care unit (ICU), surgery ward (SW) and internal medicine (IM) ward of four educational hospitals in Isfahan, Iran. A total of 80 samples were analyzed for the presence of airborne bacteria and particle levels. Results. The average level of bacteria ranged from 75-1194 CFU/m 3 . Mean particle levels were higher than class 100,000 cleanrooms in all wards. A significant correlation was observed between the numbers of 1-5 µm particles and levels of airborne bacteria in operating theatres and ICUs. The results showed that factors which may influence the airborne bacterial level in hospital environments should be properly managed to minimize the risk of HAIs especially in operating theaters. Conclusions. Microbial air contamination of hospital settings should be performed by the monitoring of airborne bacteria, but particle counting could be considered as a good operative method for the continuous monitoring of air quality in operating theaters and ICUs where higher risks of infection are suspected.

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Section: Resultsmentioning

confidence: 72%

“…2). Some studies have shown that the particle peaks are not associated with the peak of airborne bacteria, due to the presence of non-bacteria carrying particles [9,16]. The mean particle counts (particle size ≥0.5µm) were above maximum level for class 100,000 cleanroom [15] (Tab.…”

Section: Resultsmentioning

confidence: 95%

Monitoring of airborne bacteria and aerosols in different wards of hospitals – Particle counting usefulness in investigation of airborne bacteria

Mirhoseini¹,

Nikaeen²,

Khanahmd³

et al. 2015

Ann Agric Environ Med.

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“…The bacterial counts are generally higher during periods of activity and when more people are present in the operation theatre. More recent, through the use of modern, better-ventilated operation theatres (20 changes of air/h) and impermeable patient and personnel clothing, perioperative bacterial contamination may well be less (Verkkala et al, 1998). However, many surgical procedures in which implants are introduced in the body last much longer than 1 h. Therefore, the bacterial adhesion densities chosen in our experiments represent a moderate to worst case scenario.…”

Section: G Subbiahdoss Et Almentioning

confidence: 99%

Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating

Subbiahdoss

Pidhatika

Coullerez

et al. 2010

eCM

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Biomaterials-associated-infections (BAI) are serious complications in modern medicine. Although non-adhesive coatings, like polymer-brush coatings, have been shown to prevent bacterial adhesion, they do not support cell growth. Bi-functional coatings are supposed to prevent biofilm formation while supporting tissue integration. Here, bacterial and cellular responses to poly(ethylene glycol) (PEG) brush-coatings on titanium oxide presenting the integrin-active peptide RGD (arginine-glycine-aspartic acid) (bioactive "PEG-RGD") were compared to monofunctional PEG brush-coatings (biopassive "PEG") and bare titanium oxide (TiO 2 ) surfaces under flow. Staphylococcus epidermidis ATCC 35983 was deposited on the surfaces under a shear rate of 11 s -1 for 2 h followed by seeding of U2OS osteoblasts. Subsequently, both S. epidermidis and U2OS cells were grown simultaneously on the surfaces for 48 h under low shear (0.14 s -1

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“…iomaterial-associated infections (BAI) remain the number one cause of failure of biomaterial implants or devices despite the development of various strategies to control BAI during implantation, like, e.g., modern, ventilated operating theaters and impermeable personnel clothing (14). Microbial adhesion is considered to be the onset of BAI and can lead to the formation of a biofilm, in which microorganisms embed themselves in a complex matrix of extracellular polymeric substances (EPS), which provides protection against antibiotic treatment and the host immune system (5,7).…”

mentioning

confidence: 99%

Bacterial Adhesion Forces with Substratum Surfaces and the Susceptibility of Biofilms to Antibiotics

Muszanska

Nejadnik

Chen

et al. 2012

Antimicrob Agents Chemother

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bBiofilms causing biomaterial-associated infection resist antibiotic treatment and usually necessitate the replacement of infected implants. Here we relate bacterial adhesion forces and the antibiotic susceptibility of biofilms on uncoated and polymer brushcoated silicone rubber. Nine strains of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa adhered more weakly to brush-coated silicone rubber (؊0.05 ؎ 0.03 to ؊0.51 ؎ 0.62 nN) than to uncoated silicone rubber (؊1.05 ؎ 0.46 to ؊5.1 ؎ 1.3 nN). Biofilms of weakly adhering organisms on polymer brush coatings remained in a planktonic state, susceptible to gentamicin, unlike biofilms formed on uncoated silicone rubber.

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The conventionally ventilated operating theatre and air contamination control during cardiac surgery – bacteriological and particulate matter control garment options for low level contamination

Abstract: The use of clean air suits and impermeable patient clothing results in a low exogenous contamination of air and wound. Continuous air particle monitoring is a good intraoperative method to monitor the air contamination longitudinally in an operating theatre.

Cited by 49 publications

References 9 publications

Monitoring of airborne bacteria and aerosols in different wards of hospitals – Particle counting usefulness in investigation of airborne bacteria

Monitoring of airborne bacteria and aerosols in different wards of hospitals – Particle counting usefulness in investigation of airborne bacteria

Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating

Bacterial Adhesion Forces with Substratum Surfaces and the Susceptibility of Biofilms to Antibiotics

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