The Conversion Efficiency of trans-11 and trans-12 18:1 by Δ9-Desaturation Differs in Rats

Kraft, Jana; Hanske, Laura; Möckel, Peter; Zimmermann, Sindy; Härtl, Albert; Kramer, J. K. G.; Jahreis, Gerhard

doi:10.1093/jn/136.5.1209

Cited by 30 publications

(22 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ovine fat diets promoted a deposition of these minor isomers, whose biological effects are not completely understood. In human diets, the most common TFA is the t11-18:1 [4], and due to its bioconversion into c9,t11 CLA isomer by SCD, the t11-18:1 has been considered a neutral or beneficial trans isomer [13]. Butter naturally enriched in t11-18:1 and c9,t11 CLA isomer reduced lipid deposition in rabbits aorta [29].…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose, we used the average of 20% for the t11-18:1 conversion to c9,t11, reported for male Wistar rats by Kraft et al [4]. The deposition values were calculated by dividing the total amount of c9,t11 quantified in the tissue by the total amount of c9,t11 ingested (the exogenous contribution) plus 20% of t11-18:1 derived (the endogenous contribution).…”

Section: Analysis Of Fatty Acidsmentioning

confidence: 99%

“…Alternatively, in tissues, the vaccenic acid (t11-18:1), also produced in the rumen, is converted to rumenic acid via D9-desaturation by the action of stearoyl-CoA desaturase (SCD) enzyme [3]. This endogenous synthesis through vaccenic acid is also observed in other animal species, as rodents [4]. Although the majority of dietary CLA isomers in foodstuff is rumenic acid, commercial supplements with synthetic CLA often include the t10,c12 CLA isomer in equivalent proportions.…”

mentioning

confidence: 97%

See 2 more Smart Citations

Dietary CLA Combined with Palm Oil or Ovine Fat Differentially Influences Fatty Acid Deposition in Tissues of Obese Zucker Rats

Martins¹,

Lopes²,

Alves

et al. 2011

Lipids

View full text Add to dashboard Cite

The effect of dietary conjugated linoleic acid (CLA) supplementation in combination with fat from vegetable versus animal origin on the fatty acid deposition, including that of individual 18:1 and 18:2 (conjugated and non-conjugated) isomers, in the liver and muscle of obese rats was investigated. For this purpose, 32 male Zucker rats were randomly assigned to one of four diets containing palm oil or ovine fat, supplemented or not with 1% of 1:1 cis(c)9,trans(t)11 and t10,c12 CLA isomers mixture. Total fatty acid content decreased in the liver and muscle of CLA-fed rats. In the liver, CLA increased saturated fatty acids (SFA) in 11.9% and decreased monounsaturated fatty acids (MUFA) in 6.5%. n-3 Polyunsaturated fatty acids (PUFA) relative proportions were increased in 30.6% by CLA when supplemented to the ovine fat diet. In the muscle, CLA did not affect SFA but decreased MUFA and PUFA percentages. The estimation of Δ9-indices 16 and 18 suggested that CLA inhibited the stearoyl-CoA desaturase activity in the liver (a decrease of 13-38%), in particular when supplemented to the ovine fat diet. Concerning CLA supplementation, the t10,c12 isomer percentage was 60-80% higher in the muscle than in the liver. It is of relevance that rats fed ovine fat, containing bio-formed CLA, had more c9,t11 CLA isomer deposited in both tissues than rats fed palm oil plus synthetic CLA. These results highlight the importance to further clarify the biological effects of consuming foods naturally enriched in CLA, alternatively to CLA dietary supplementation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Fatty Acidsmentioning

confidence: 99%

mentioning

confidence: 97%

See 1 more Smart Citation

Dietary CLA Combined with Palm Oil or Ovine Fat Differentially Influences Fatty Acid Deposition in Tissues of Obese Zucker Rats

Martins¹,

Lopes²,

Alves

et al. 2011

Lipids

View full text Add to dashboard Cite

show abstract

“…Indeed, trans-11 was found to give rise to cis-9, trans-11-C 18:2 (as a conjugated linoleic acid, CLA) through D-9 desaturation in liver microsomes, while under the same experimental conditions trans-9 stayed unchanged [49]. In whole rats, dietary trans-11 was also transformed into cis-9, trans-11-C 18:2 in most organs [50]. This CLA added to the diet of pigs was abundantly recovered in liver PL [51].…”

Section: Esterification Of Isomers Into Tag and Pl Of Liver Cellsmentioning

confidence: 97%

Vaccenic and Elaidic Acid Equally Esterify into Triacylglycerols, but Differently into Phospholipids of Fed Rat Liver Cells

Degrace

Gresti

et al. 2011

Lipids

View full text Add to dashboard Cite

Elaidic acid (trans-9-C₁₈:₁ or trans-9) is assumed to exert atherogenic effects due to its double bond configuration. The possibility that trans-9 and vaccenic acid (trans-11-C₁₈:₁ or trans-11), its positional isomer, were biochemically equivalent and interchangeable compounds, was investigated by reference to their cis-isomers through esterification-related activities using rat liver cells and subcellular fractions. In hepatocytes, both trans-C₁₈:₁ were incorporated to the same extent in triacylglycerols, but trans-9 was more esterified than trans-11 into phospholipids (P < 0.05). Glycerol-3-phosphate acyltransferase activity in microsomes was lower with trans-11 than with trans-9, while this activity in mitochondria was ~40% greater with trans-11 than with trans-9 (P < 0.05). Activity of 2-lysophosphatidic acid acyltransferase in microsomes was of comparable extent with both trans isomers, but activity of 2-lysophosphatidylcholine acyltransferase was significantly greater with trans-9 than with trans-11 at P < 0.01. Lipoproteins secreted by hepatocytes reached equivalent levels in the presence of any isomers, but triacylglycerol production was more elevated with trans-11 than with trans-9 at P < 0.05. Cholesterol efflux from previously labelled hepatocytes was lower with trans-11 than with trans-9. When these cells were exposed to either trans-C₁₈:₁, the gene expression of proteins involved in fatty acid esterification and lipoprotein synthesis was unaffected, which indicates that the biochemical differences essentially depended on enzyme/substrate affinities. On the whole, vaccenic and elaidic acid were shown to incorporate cell phospholipids unequally, at least in vitro, which suggests they can differently affect lipid metabolic pathways in normal cells.

show abstract

“…enzymatic transformation) in the rumen of animals (e.g., bacterian flora in cows, sheeps, goats) in mammalian breasts; 4,5 (ii) partial (or total) industrial catalysis of vegetables or fish oils requiring hydrogen gas or a metal catalyst (e.g. nickel) to replace saturated FAs, solidify vegetal fat products (e.g.…”

mentioning

confidence: 99%

The Basic of Trans-Fatty Acids in Foods: An Update to Prevent Diseases

Menaa¹

2015

Adv Food Technol Nutr Sci Open J

View full text Add to dashboard Cite

show abstract

The Conversion Efficiency of trans-11 and trans-12 18:1 by Δ9-Desaturation Differs in Rats

Cited by 30 publications

References 44 publications

Dietary CLA Combined with Palm Oil or Ovine Fat Differentially Influences Fatty Acid Deposition in Tissues of Obese Zucker Rats

Dietary CLA Combined with Palm Oil or Ovine Fat Differentially Influences Fatty Acid Deposition in Tissues of Obese Zucker Rats

Vaccenic and Elaidic Acid Equally Esterify into Triacylglycerols, but Differently into Phospholipids of Fed Rat Liver Cells

The Basic of Trans-Fatty Acids in Foods: An Update to Prevent Diseases

Contact Info

Product

Resources

About