The conversion of β-carotene to vitamin A in adipocytes drives the anti-obesogenic effects of β-carotene in mice

Coronel, Johana; Yu, Jianshi; Pilli, Nageswara; Kane, Maureen A.; Amengual, Jaume

doi:10.1016/j.molmet.2022.101640

Cited by 15 publications

(11 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We recently over-expressed BCO1 in the adipose tissue of Bco1 -/mice fed β-carotene. Under these conditions, we observed an increase in vitamin A levels including retinoic acid, and a reduction of adipose tissue size [16]. These effects are in line with those reported by Palou's group utilizing both dietary vitamin A and retinoic acid supplementation, which promotes fatty acid oxidation and thermogenesis in various experimental models [61][62][63][64][65][66][67][68][69][70][71].…”

Section: Discussionsupporting

confidence: 91%

“…In 2011, we reported that wild-type mice fed β-carotene exhibited a reduction in adipose tissue size. Bco1 -/- mice subjected to the same experimental conditions did not chow differences in adipose tissue size in comparison to control-fed mice despite accumulating large amounts of β-carotene in this tissue [16]. We recently over-expressed BCO1 in the adipose tissue of Bco1 -/- mice fed β-carotene.…”

Section: Discussionmentioning

confidence: 99%

“…Upon absorption, provitamin A carotenoids are cleaved by the action of β-carotene oxygenase 1 (BCO1), the limiting enzyme in vitamin A formation [10]. Our data show that the enzymatic activity of BCO1 mediates the bioactive actions of β-carotene in various preclinical models of obesity and atherosclerosis [11–16]. We showed that the dietary supplementation with β-carotene delays atherosclerosis progression by reducing cholesterol hepatic secretion in low-density lipoprotein receptor (LDLR)-deficient ( Ldlr - /- ) mice [15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

β-carotene accelerates the resolution of atherosclerosis in mice

Pinos

Coronel

Albakri

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

β-carotene oxygenase 1 (BCO1) catalyzes the cleavage of β-carotene to form vitamin A. Besides its role in vision, vitamin A regulates the expression of genes involved in lipid metabolism and immune cell differentiation. BCO1 activity is associated with the reduction of plasma cholesterol in humans and mice, while dietary β-carotene reduces hepatic lipid secretion and delays atherosclerosis progression in various experimental models. Here we show that β-carotene also accelerates atherosclerosis resolution in two independent murine models, independently of changes in body weight gain or plasma lipid profile. Experiments inBco1-/-mice implicate vitamin A production in the effects of β-carotene on atherosclerosis resolution. To explore the direct implication of dietary β-carotene on regulatory T cells (Tregs) differentiation, we utilized anti-CD25 monoclonal antibody infusions. Our data show that β-carotene favors Treg expansion in the plaque, and that the partial inhibition of Tregs mitigates the effect of β-carotene on atherosclerosis resolution. Our data highlight the potential of β-carotene and BCO1 activity in the resolution of atherosclerotic cardiovascular disease.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

β-carotene accelerates the resolution of atherosclerosis in mice

Pinos

Coronel

Albakri

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…When upregulated, Bco1 boosts lipid metabolism and impacts lipid homeostasis. 28,29 In particular, Bco1 directly oversees the esterification or formation of lipids such as cholesterol esters and phospholipids. 30 This function is crucial for the development of hepatic lipid metabolism and the maintenance of cholesterol homeostasis in R. pusillus.…”

Section: Qrt-pcr Experimental Verification Resultsmentioning

confidence: 99%

“…Bco1 (β‐carotene oxygenase 1) plays a significant role in cholesterol metabolism. When upregulated, Bco1 boosts lipid metabolism and impacts lipid homeostasis 28,29 . In particular, Bco1 directly oversees the esterification or formation of lipids such as cholesterol esters and phospholipids 30 .…”

Section: Discussionmentioning

confidence: 99%

Adapting to stress: The effects of hibernation and hibernacula temperature on the hepatic transcriptome of Rhinolophus pusillus

Wang,

Chen

et al. 2024

The FASEB Journal

View full text Add to dashboard Cite

Hibernation, a survival strategy in mammals for extreme climates, induces physiological phenomena such as ischemia–reperfusion and metabolic shifts that hold great potential for advancements in modern medicine. Despite this, the molecular mechanisms underpinning hibernation remain largely unclear. This study used RNA‐seq and Iso‐seq techniques to investigate the changes in liver transcriptome expression of Rhinolophus pusillus during hibernation and active periods, as well as under different microhabitat temperatures. We identified 11 457 differentially expressed genes during hibernation and active periods, of which 395 showed significant differential expression. Genes associated with fatty acid catabolism were significantly upregulated during hibernation, whereas genes related to carbohydrate metabolism and glycogen synthesis were downregulated. Conversely, immune‐related genes displayed differential expression patterns: genes tied to innate immunity were significantly upregulated, while those linked to adaptive immunity and inflammatory response were downregulated. The analysis of transcriptomic data obtained from different microhabitat temperatures revealed that R. pusillus exhibited an upregulation of genes associated with lipid metabolism in lower microhabitat temperature. This upregulation facilitated an enhanced utilization rate of triglyceride, ultimately resulting in increased energy provision for the organism. Additionally, R. pusillus upregulated gluconeogenesis‐related genes regardless of the microhabitat temperature, demonstrating the importance of maintaining blood glucose levels during hibernation. Our transcriptomic data reveal that these changes in liver gene expression optimize energy allocation during hibernation, suggesting that liver tissue adaptively responds to the inherent stress of its function during hibernation. This study sheds light on the role of differential gene expression in promoting more efficient energy allocation during hibernation. It contributes to our understanding of how liver tissue adapts to the stressors associated with this state.

show abstract

Effects of dietary β‐carotene on color and flavor quality of ovaries in adult female Chinese mitten crab (Eriocheir sinensis)

Zhang,

Jiang

et al. 2024

eFood

View full text Add to dashboard Cite

This study aimed to investigate the effects of adding different levels (0, 50, 100, 200 and 300 mg/kg) of dietary β‐carotene on the color and flavor quality of adult female E. sinensis ovaries. Adding moderate amount of β‐carotene to the feed could regulate the distribution of carotenoids, fatty acids, and free amino acids in the ovaries of E. sinensis, as well as increase the content of total carotenoids, total fatty acids, sweet and umami amino acids in the ovaries. The dietary β‐carotene have limited coloring effects and a significant impact on the flavor of the ovaries. During thermal process, the addition of 100 mg/kg β‐carotene could effectively promote formation of aroma precursors and generate more characteristic aroma components (2‐methylbutanal, 3‐methylbutanal, hexanal, benzaldehyde, methylpyrazine). However, excessive β‐carotene supplementation (300 mg/kg) led to adverse effects on physiological metabolism and flavor of E. sinensis ovaries. Considering both feed cost and quality improvement, β‐carotene supplementation at 100 mg/kg can be considered the optimal feed supplementation for adult female E. sinensis within a 70‐day rearing period to improve edible quality of ovaries.

show abstract

The conversion of β-carotene to vitamin A in adipocytes drives the anti-obesogenic effects of β-carotene in mice

Cited by 15 publications

References 62 publications

β-carotene accelerates the resolution of atherosclerosis in mice

β-carotene accelerates the resolution of atherosclerosis in mice

Adapting to stress: The effects of hibernation and hibernacula temperature on the hepatic transcriptome of Rhinolophus pusillus

Effects of dietary β‐carotene on color and flavor quality of ovaries in adult female Chinese mitten crab (Eriocheir sinensis)

Contact Info

Product

Resources

About