As with many species of seahorses, Hippocampus hippocampus wild populations are being subjected to uncontrolled exploitation in their natural environment. Thus, aquaculture could contribute to satisfy the commercial demand for animals while promoting the recovery of wild stocks. The present study was conducted to compare the e¡ect of the substituting Artemia nauplii with rotifers for ¢rst feeding seahorse juveniles. Survival, growth and biochemical composition of prey organisms and ¢sh were studied during the feeding trial. In addition, to help the biometric study, an anaesthetic test was also carried out using clove oil. The results showed excellent survival (average 60%) in juveniles exclusively fed with Artemia, with better values than those reported previously obtained by other authors for this species. By comparison, high mortality and poor growth were observed during ¢rst feeding with seahorses fed on rotifers. This could have been related to the lower energy intake and poorer nutritional value of the rotifers. Furthermore, clove oil concentrations of 25 ppm were found to work well as an anaesthetic for seahorse juveniles. Overall, ¢rst feeding Artemia alone was found to be an e⁄cient and simpli¢ed method for feeding young H. hippocampus fry, building the principles for their culture for ornamental or restocking purposes.