1996
DOI: 10.1074/jbc.271.6.2995
|View full text |Cite
|
Sign up to set email alerts
|

The COQ7 Gene Encodes a Protein in Saccharomyces cerevisiae Necessary for Ubiquinone Biosynthesis

Abstract: Ubiquinone (coenzyme Q) is a lipid that transports electrons in the respiratory chains of both prokaryotes and eukaryotes. Mutants of Saccharomyces cerevisiae deficient in ubiquinone biosynthesis fail to grow on nonfermentable carbon sources and have been classified into eight complementation groups (coq1 coq8; Tzagoloff, A., and Dieckmann, C. L.(1990) Microbiol. Rev. 54, 211-225). In this study we show that although yeast coq7 mutants lack detectable ubiquinone, the coq7 1 mutant does synthesize demethoxyubiq… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

5
159
0
3

Year Published

1996
1996
2024
2024

Publication Types

Select...
9

Relationship

3
6

Authors

Journals

citations
Cited by 177 publications
(167 citation statements)
references
References 48 publications
5
159
0
3
Order By: Relevance
“…In contrast, both the clk-1(e2519) and the clk-1(qm30) animals failed to transport Q 7 as efficiently to the mitochondria as Q 8 (Fig. 4, D and F).…”
Section: Clk-1 Fed Varying Q Isoforms: Fertility and Quinone Analysismentioning
confidence: 88%
See 1 more Smart Citation
“…In contrast, both the clk-1(e2519) and the clk-1(qm30) animals failed to transport Q 7 as efficiently to the mitochondria as Q 8 (Fig. 4, D and F).…”
Section: Clk-1 Fed Varying Q Isoforms: Fertility and Quinone Analysismentioning
confidence: 88%
“…The diet-derived Q 8 is present in mitochondria isolated from clk-1 mutant adults; however, it is important to note that the amount of Q 8 assimilated is far lower than the amount of Q 9 produced by N2 (16). We considered that uptake of the shorter Q 8 isoform derived from the diet may contribute to the Clk-1 phenotypes, because the worms normally synthesize the Q 9 isoform.…”
mentioning
confidence: 99%
“…Cells the were harvested after 3 days of incubation at 30°C with shaking (200 rpm, A 600 nm ϭ 10), and the lipids were extracted as described (29). The lipid extracts were concentrated, the volume was adjusted to 1 ml with hexane, and 0.10-ml aliquots (5.0 -12.0 ϫ 10 3 cpm) were analyzed by normal phase HPLC employing a cyanopropyl column (Zorbax, 5 m, 4.6 ϫ 250 mm; MacMod Analytical, Chadds Ford, PA) as described (30).…”
Section: Figmentioning
confidence: 99%
“…5; Refs. [5][6][7][8][9]. Yeast mutants in the COQ1, COQ2, COQ3, and COQ7 genes have been characterized (7,8,10,11).…”
mentioning
confidence: 99%