The correlation between clinical characteristics of preeclampsia and the concentration of fetal DNA in maternal circulation

Engel, Karina; Płonka, Tomasz; Bilar, Marek; Orzińska, Agnieszka; Brojer, Ewa; Ronin-Walknowska, Elżbieta

doi:10.1016/j.ejogrb.2007.04.008

Cited by 6 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The circulating cell‐free DNA (cfDNA) in maternal plasma is a mixture of placental and maternal DNA 9 . There are many differences between maternal and fetal cfDNA, including differences in genetic factors that allow cfDNA to be used for fetal sex determination, 10 non‐invasive fetal RhD genotyping, 11 and pregnancy‐related disorder screening (e.g., for pre‐eclampsia, 12 chromosomal disorders, and monogenic genetic disorders 13 ). Maternal and fetal cfDNA molecules have different length distributions 14‐16 ; fetal DNA molecules are usually shorter than DNA molecules of maternal origin.…”

Section: Introductionmentioning

confidence: 99%

Simulated confined placental mosaicism proportion (SCPMP) based on cell‐free fetal DNA fraction enrichment can reduce false‐positive results in non‐invasive prenatal testing

et al. 2022

View full text Add to dashboard Cite

Objective To decrease the false‐positive rate of NIPT using cell‐free fetal DNA (cffDNA) fraction enrichment and the simulated confined placental mosaicism proportion (SCPMP) threshold application via cffDNA quantification. Method Using a cffDNA enrichment method, 303 plasma samples with positive NIPT results (Z‐score > 3.0; 200 true‐positive and 103 false‐positive cases) were re‐sequenced. A method to calculate the SCPMP based on the quantified cffDNA fraction was developed; the SCPMP threshold between true‐ and false‐positive NIPT results was determined and used for re‐analyses. Results With enrichment, the fetal fraction of the 303 samples was 26.9 ± 8.4%, compared to 11.0 ± 3.2% without enrichment. The optimized threshold method with double determination using the Z‐value‐defined SCPMP can reduce the false‐positive rates for trisomies 21, 18, and 13 by 87%, 80%, and 88.9%, respectively. Conclusion Our optimized method can decrease the false‐positive rate of NIPT results.

show abstract

Section: Introductionmentioning

confidence: 99%

Simulated confined placental mosaicism proportion (SCPMP) based on cell‐free fetal DNA fraction enrichment can reduce false‐positive results in non‐invasive prenatal testing

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The concentration of free fetal DNA is higher in pre-eclamptic pregnancies [11]. There is a correlation between fetal DNA levels and severity of symptoms [12]. The dynamics of fetal DNA concentrations during pregnancy is rather complex, suggested to be because of placental necrosis or apoptosis and also as a result of decreased DNA elimination [13].…”

Section: Introductionmentioning

confidence: 99%

Hypermethylated RASSF1A in maternal plasma as a non-sex-dependent marker for monitoring of pre-eclampsia

El-Garf¹,

Nasr²,

Bibers³

et al. 2013

Medical Research Journal

View full text Add to dashboard Cite

Objective Our aim is to investigate whether the quantification of cell-free fetal DNA (cffDNA) using the RASSF1A system will be effective for the detection of severity of pre-eclampsia. Materials and methodsWe performed real-time PCR after methylation-sensitive restriction enzyme digestion to detect placental-derived RASSF1A sequences (fetal DNA) in the plasma of 120 pregnant women.Results Hypermethylated RASSF1A sequences were detectable in the plasma of all 120 pregnant women. There was an increase in the level of hypermethylated RASSF1A (cffDNA) in mild and severe pre-eclampsia.Conclusion Hypermethylated RASSF1A is a non-sexdependent marker for predicting pre-eclampsia and can be a useful marker for severity.

show abstract

Circulating Nucleic Acids in Maternal Plasma and Serum in Pregnancy Complications: Are They Really Useful in Clinical Practice? A Systematic Review

et al. 2020

View full text Add to dashboard Cite

The correlation between clinical characteristics of preeclampsia and the concentration of fetal DNA in maternal circulation

Cited by 6 publications

References 5 publications

Simulated confined placental mosaicism proportion (SCPMP) based on cell‐free fetal DNA fraction enrichment can reduce false‐positive results in non‐invasive prenatal testing

Simulated confined placental mosaicism proportion (SCPMP) based on cell‐free fetal DNA fraction enrichment can reduce false‐positive results in non‐invasive prenatal testing

Hypermethylated RASSF1A in maternal plasma as a non-sex-dependent marker for monitoring of pre-eclampsia

Circulating Nucleic Acids in Maternal Plasma and Serum in Pregnancy Complications: Are They Really Useful in Clinical Practice? A Systematic Review

Contact Info

Product

Resources

About