The correlation between immunohistochemical expression of MMP-2 and the prognosis of epithelial ovarian cancer

Ekinci, Tekin; Özbay, Pelin Özün; Yiğit, Seyran; Yavuzcan, Ali; Uysal, Selda; Soylu, Ferit

doi:10.17772/gp/1702

Cited by 14 publications

(15 citation statements)

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“…MMP-2 is a matrix metalloproteinase (MMP) family protein that can degrade the extracellular matrix and play a crucial role in physiological and pathological processes such as migration and invasion of malignant tumours [26]. Elevated MMP-2 expression has been discovered in human epithelial ovarian cancer, and it has been suggested that human epithelial ovarian cancer tissue may be inherently more aggressive and metastatic [27]. It is now well believed that MMP-2 may be involved in the process of tumour cell invasion and metastasis [28].…”

Section: Discussionmentioning

confidence: 99%

T-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistanceT-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistance

Guan

Lin

Zhang

et al. 2020

Preprint

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Background: T-cadherin plays a crucial role in maintaining normal tissue structure by regulating specific cell adhesion, cellular recognition and signal transduction. The purpose of this study was to evaluate whether T-cadherin influences epithelial ovarian cancer (EOC) progression, differentiation and drug resistance and its possible mechanisms.Methods: Epithelial ovarian carcinoma (EOC, n=63) and relevant contralateral normal ovarian (CNO, n=41) fresh tissues were collected from epithelial ovarian carcinoma patients, and benign ovarian tumour fresh tissues were collected from 55 patients with benign ovarian tumours. The human epithelial ovarian carcinoma cell line A2780 was cultured. T-cadherin expression was assessed by real-time RT-PCR and Western blotting, and the expression of matrix metalloproteinase-2 (MMP-2) was detected by Western blotting. pcDNA‑T‑cad plasmid technology was used to upregulate T-cadherin expression. In addition, A2780 cell migration and invasion ability, viability, colony formation, proliferation, apoptosis and sensitivity to paclitaxel were measured.Results: T‑cadherin mRNA and protein expression in EOC tissues from EOC patients was significantly downregulated, and there was no significant difference between the matched contralateral normal ovarian fresh tissue from the same patient and the benign ovarian tumour tissues from other patients. The migration and invasion abilities, viability, colony formation, and proliferation were attenuated by restoration of T‑cadherin expression in A2780 cells via pcDNA‑T‑cad transfection; apoptosis, MMP-2 expression and sensitivity to Taxol were also enhanced by restored T‑cadherin expression. The T‑cadherin expression level was well correlated with the clinical characteristics and symptoms of EOC patients, including tumour stage, histology, lymph node metastasis, tumour size, distant metastasis and cisplatin resistance.Conclusions: T‑cadherin participates in the processes of epithelial ovarian carcinoma cell migration, invasion, proliferation, apoptosis and sensitivity to paclitaxel and can regulate the expression of MMP-2. Downregulation of T‑cadherin expression may contribute to epithelial ovarian cancer progression, differentiation and drug resistance.

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Section: Discussionmentioning

confidence: 99%

T-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistanceT-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistance

Guan

Lin

Zhang

et al. 2020

Preprint

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“…This meta-analysis, which combined results from 8 studies of 965 patients, explores the association of tumor-derived MMP-2 and stromal-derived MMP-2 with ovarian cancer prognosis. 14 , 16 , 17 , 19 – 21 , 25 , 26 We demonstrated that the expression of MMP-2 in cancer cells was associated with a significant reduction of overall survival rate (HR, 1.52; 95% CI, 1.06–2.20), whereas the expression of MMP-2 in stromal cells did not appear to have an overall influence on the patients survival rate of ovarian cancer (HR, 1.28; 95% CI, 0.56–2.92).…”

Section: Discussionmentioning

confidence: 99%

The Expression of Tumor-Derived and Stromal-Derived Matrix Metalloproteinase 2 Predicted Prognosis of Ovarian Cancer

Fu¹,

et al. 2015

International Journal of Gynecological Cancer

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ObjectiveTo evaluate the association of tumor-derived matrix metalloproteinase 2 (MMP-2) and stromal-derived MMP-2 expression with the prognosis of patients with ovarian cancer, a meta-analysis study was performed, which was aimed to comprehensively review the evidence of MMP-2 as prognostic biomarkers in ovarian cancers.MethodsAll relevant studies were searched in PubMed and Web of Science until May 30, 2014. Hazard ratios (HRs) with their 95% confidence intervals (CIs) were used to assess the association between MMP-2 expression (tumor-derived or stromal-derived) and prognosis of patients with ovarian cancer. Pooled odds ratios (ORs) and their 95% CIs were used to assess the correlation of MMP-2 expression with the clinicopathological features of patients with ovarian cancer.ResultsA total of 965 patients in 8 studies were included in this analysis. Among them, tumor-derived and stromal-derived MMP-2 expression was detected in 7 and 5 articles, respectively. The results revealed that ovarian cancer patients with positive tumor-derived MMP-2 expression showed a worse prognosis than did the ones with negative tumor-derived MMP-2 expression (HR, 1.52; 95% CI, 1.06–2.20). However, ovarian cancer patients with positive stromal-derived MMP-2 expression had not. In addition, we also found that tumor-derived MMP-2 expression was associated with distant metastasis (absent vs present; pooled OR, 4.52; 95% CI, 1.56–13.09; P = 0.001).ConclusionsThese results suggested that positive tumor-derived MMP-2 expression could predict a lower overall survival rate and could be an independent dangerous prognostic factor in patients with ovarian cancer.

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“…Высокий уровень ММР-2 и MMP-9 в плазме крови у больных колоректальным раком и раком желудка коррелирует с метастазами в лимфатические узлы [92]. Сверхэкспрессия ММР-2 связана с плохим прогнозом у больных немелкоклеточным раком лёгких и раком яичников [93].…”

Section: роль тетраспанинов и протеаз экзосом в прогрессии злокачествunclassified

The role of exosomal tetraspanins and proteases in tumor progression

et al. 2018

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Major (CD9, CD63, CD81) and others (CD82, CD151, Tspan8) tetraspanins are widely represented in exosomes, where they interact with various proteins and form functional tetraspanin complexes. Tetraspanin complexes include proteases. Tetraspanin-associated exosomal proteases (ADAM proteases, MMPs, EMMPRIN) play an important role in the processes of cell motility, migration, invasion and formation of metastases. Also, a significant contribution to tumor progression is made by proteases that are not associated with tetraspanins. They destabilize intercellular contacts, promote migration and invasion of tumor cells, participate in the regulation of the expression IGF-I, VEGF and transcription factors activation/deactivation. The role of other proteases of exosomes in the processes of tumor progression is being clarified.

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The correlation between immunohistochemical expression of MMP-2 and the prognosis of epithelial ovarian cancer

Cited by 14 publications

References 0 publications

T-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistanceT-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistance

T-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistanceT-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistance

The Expression of Tumor-Derived and Stromal-Derived Matrix Metalloproteinase 2 Predicted Prognosis of Ovarian Cancer

The role of exosomal tetraspanins and proteases in tumor progression

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