The correlation between spermatozoa morphometry and the testosterone levels in the epididymis of aceh cow

Abstract

The objective of this study was to determine the correlation between spermatozoa morphometry and testosterone concentration in the epididymal caput, corpus, and cauda of aceh cattle. The epididymal sperm swab slides were stained using Williams's staining to examined the morphology and morphometry of spermatozoa whereas the level of testosterone from epididymal caput, corpus, and cauda extract was measured using enzyme-linked immunosorbent assay (ELISA). The data of sperm morphology were presented descriptively whereas the data of sperm morphometry and testosterone concentration were analyzed using one way ANOVA. Pearson correlation test was conducted to know the correlation between the total length of spermatozoa (TLS) and testosterone concentration in three parts of epididymis. The result showed that the sperm morphology in caput, corpus, and cauda of epididymis was not different statistically. The TLS of aceh cattle at caput, corpus, and cauda epididymis were 69,70±0,8 µm, 70,90±0.39 µm, and 72,98±0.74 µm respectively and statistically different (p<0.05). In addition, the concentration of testosterone in caput, corpus, and cauda epididymis were 9,92±3,76 ng/g, 16.96±13,22 ng/g, and 15,63±11.80 ng/g respectively and also not different among three parts of epididymis (p>0.05). The positive correlation (r= 0.413, p= 0.416) was found between TLS and testosterone concentration at caput whereas in corpus and cauda those correlation were negative and not different (corpus: r= -0.638, p= 0.172 and cauda: r= -0.719, p= 0,108). In conclusion, the longest-sized spermatozoa found in the cauda epididymis are mature spermatozoa where the maturation process optimally takes place in caput epididymis. Testosterone found in the epididymis of aceh cow can be used as an alternative source of testosterone.

Identification and Determination of the Seminiferous Epithelium Stages and Spermatid Development in the Testis of Aceh Bull (Bos indicus)

“…The data regarding spermatogenesis and spermiogenesis in the testicular tissue of Aceh bull obtained in this study are basic data that complements the previous data regarding correlation between spermatozoa morphometric and testosterone level during the maturation process in the epididymis (caput, corpus, and cauda) which have been reported by Wahyuni et al [ 45 ]. Therefore, further study on the reproductive aspects of Aceh bull is needed to determine the potential of these cattle as producers of good quality of semen to be inseminated in Aceh cows.…”

Effect of Rosemary Essential Oil Oral Supplementation on Testes Morphometric Measures, Accessory Genital Glands, and Semen Parameters in the Barki Rams

“…It also resulted in a positive correlation between testosterone concentration, testicular parameter measures, epididymal tail, and semen quality. At the same time, the rosemary oil's effect on testosterone levels is critical because the testosterone hormone is essential for sperm cell maturation and the motility of mature spermatozoa before they enter the ductus deferens and stimulate some duct cells (Wahyuni et al, 2020).…”

Evaluasi Validitas Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit dan Waktu Sentrifugasi Sampel Darah untuk Pengukuran Konsentrasi Hormon Kortisol pada Kambing Kacang

“…Ketika tubuh mendapatkan stressor, poros hipotalamuspituitari-adrenal (HPA) akan mensekresi berbagai hormon untuk proses homeostatis tubuh diawali dengan sekresi hormone corticotropic releasing hormone (CRH) oleh hipotalamus. Pelepasan CRH kemudian akan memberikan stimulus ke kelenjar hipofisa bagian anterior untuk menghasilkan adrenocorticothropic hormone (ACTH) yang akhirnya menstimulus kelenjar adrenal bagian korteks melepaskan hormon kortisol (Sheriff et al, 2011 Uji validasi penggunaan human ELISA kit untuk hewan juga telah berhasil dan dilaporkan oleh beberapa peneliti sebelumnya baik untuk pengukuran kortisol (Lendrawati et al, 2019), maupun hormon steroid lain seperti testosteron (Wahyuni et al, 2020);Akmal et al, 2019), progesteron (Adam et al, 2017, dan estradiol (Adam et al, 2019). Ini membuktikan bahwa human ELISA kit juga dapat diaplikasikan pada hewan.…”