The correlation of combined OGG1, CYP1A1 and GSTP1 gene variants and risk of lung cancer of male Iraqi waterpipe tobacco smokers

Abstract

Genetic polymorphisms of genes whose products are responsible for activities, such as xenobiotic metabolism, mutagen detoxification and DNA-repair, have been predicted to be associated with the risk of developing lung cancer (LC). The association of LC with tobacco smoking has been extensively investigated, but no studies have focused on the Arab ethnicity. Previously, we examined the association between genetic polymorphisms among Phase I and Phase II metabolism genes and the risk of LC. Here, we extend the data by examining the correlation of OGG1 Ser326Cys combined with CYP1A1 (Ile462Val and MspI) and GSTP1 (Ile105Val and Ala103Val) polymorphisms with the risk of LC. Polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) and gene sequencing were carried out for genotyping the OGG1 polymorphisms of 123 LC patients and 129 controls. No significant differences in the frequencies of the OGG1 mutant allele between patients and controls were found. The distributions of heterozygous Ser/Cys or Cys/Cys genotypes of OGG1 were not associated with the risk of LC either according to the histological types of LC or based on waterpipe tobacco (WP) smoking status. In contrast, the combined effect of OGG1 variants with CYP1A1 and GSTP1 variants revealed a significant correlation with the OGG1 Ser326Cys-CYP1A1 MspI variants pairing. This association was significant (p = 0.001) in individuals who carried homozygous or heterozygous variant type genotypes of both genes in a reference with carriers of both wild-type genotypes (wt/wt − wt/wt). The odds ratios were 2.99 (95% CI 1.67-5.36), 2.68 (95% CI 1.08-6.62), and 2.80 (95% CI 1.18-6.69) for those who carried (wt/wt − wt/vt + vt/vt), (wt/vt + vt/vt − wt/wt), and (wt/vt + vt/vt − wt/vt + vt/ vt), respectively. The study suggests a limited correlation is present between carrying OGG1 Ser326Cys polymorphism and the risk of developing LC in Arab populations.

A metabolism-related gene signature for predicting the prognosis in thyroid carcinoma

“…It has been published that via preventing PTEN and stimulating β-Catenin and Akt pathways, the AhR/CYP1A1 signaling pathway affected the phenotypes of breast cancer stem cells, such as growth, development, renewal, and chemoresistance ( Al-Dhfyan et al, 2017 ). Recent research has covered the genetic polymorphisms of CYP1A1 carried weight with the risk of developing cancers containing lung cancer ( Kudhair et al, 2020 ), upper digestive tract cancer ( Tian et al, 2019 ; Zhao et al, 2019 ), and thyroid carcinoma ( Bufalo et al, 2006 ; Figlioli et al, 2016 ). In thyroid carcinoma patients, CYP1A1 could serve as a prognostic marker to point to the grade of tumor severity, with the CYP1A1 gene expression associated with tumor size, the presence of metastasis, and advanced clinical stage ( GallegosVargas et al, 2016 ).…”

Investigation of the association between polymorphisms in DNA repair enzymes and STEMI

“…This alteration leads to a substitution of serine with cysteine at codon 326, commonly referred to as Ser326Cys. It has been suggested that this genetic variation can alter the function of OGG1 and potentially increase susceptibility to certain diseases [10,11].…”

The CHRNA5 polymorphism (rs16969968) and its association with waterpipe smoking addiction among Jordanians

“…In some countries, the rate of waterpipe use has surpassed that of cigarettes (Jawad et al, 2018). Waterpipe smoking is associated with several health problems (Reyes-Caballero et al, 2020;Sullman, Gras, Kagialis, Papageorgi, & Font-Mayolas, 2020), including cardiovascular diseases (Alomari, Khabour, Alzoubi, & Eissenberg, 2020;Nemmar et al, 2020), respiratory diseases (Kudhair, Alabid, Zayed, Lafta, & Taheri-Kafrani, 2020;Nemmar, Al-Salam, Beegam, Yuvaraju, & Ali, 2019), and cancer (Kudhair et al, 2020;Sabi et al, 2020).…”