Opportunity was launched in 2004 and has been providing interesting data from Mars till 2018. Meridiani Planum was the landing site for the robot. This crater has numerous rock outcrops, which are considered a valuable geological resource that contains keys to the Martian past. In this work, several algorithms have been developed for detecting the possible presence of humidity and vegetation on Mars through the images sent by the Mars Exploration Rover - B Opportunity and by the Viking Orbiter between 1976 and 1980. For this, it was carried out a sedimentary simulation of the study area, as well as an analysis of all the images from the spectral signatures extracted. The results show the existence of three types of water on the surface, as well as concentrations of Neoxanthin, also on landing area surface, that suggest the possible existence of microalgae.