The cost of muscle power production: muscle oxygen consumption per unit work increases at low temperatures in<i>Xenopus laevis</i>Daudin

Seebacher, Frank; Tallis, Jason; James, Rob S.

doi:10.1242/jeb.101147

Cited by 19 publications

(22 citation statements)

References 50 publications

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“…Isolated gastrocnemius muscle from the African clawed frog Xenopus laevis used more oxygen at low (15°C) than at high (25°C) acute temperatures per Joule of work performed regardless of acclimation conditions. The reason for this difference is at least partly that the stiffness of the muscle is greater at low temperature and therefore requires greater cross-bridge activity to achieve force production [38]. If this were a general pattern among amphibians, it could explain the patterns we observed in Lim.…”

Section: Discussionmentioning

confidence: 86%

Embryonic Developmental Temperatures Modulate Thermal Acclimation of Performance Curves in Tadpoles of the Frog Limnodynastes peronii

Seebacher

Grigaltchik

2014

PLoS ONE

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Performance curves of physiological rates are not fixed, and determining the extent to which thermal performance curves can change in response to environmental signals is essential to understand the effect of climate variability on populations. The aim of this study was to determine whether and how temperatures experienced during early embryonic development affect thermal performance curves of later life history stages in the frog Limnodynastes peronii. We tested the hypotheses that a) the embryonic environment affects mean trait values only; b) temperature at which performance of tadpoles is maximal shifts with egg incubation temperatures so that performance is maximised at the incubation temperatures, and c) incubation temperatures modulate the capacity for reversible acclimation in tadpoles. Growth rates were greater in warm (25°C) compared to cold (15°C) acclimated (6 weeks) tadpoles regardless of egg developmental temperatures (15°C or 25°C, representing seasonal means). The breadth of the performance curve of burst locomotor performance (measured at 10, 15, 20, 25, and 30°C, representing annual range) is greatest when egg developmental and acclimation temperatures coincide. The mode of the performance curves shifted with acclimation conditions and maximum performance was always at higher temperatures than acclimation conditions. Performance curves of glycolytic (lactate dehydrogenase activities) and mitochondrial (citrate synthase and cytochrome c oxidase) enzymes were modulated by interactions between egg incubation and acclimation temperatures. Lactate dehydrogenase activity paralleled patterns seen in burst locomotor performance, but oxygen consumption rates and mitochondrial enzyme activities did not mirror growth or locomotor performance. We show that embryonic developmental conditions can modulate performance curves of later life-history stages, thereby conferring flexibilty to respond to environmental conditions later in life.

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Section: Discussionmentioning

confidence: 86%

Embryonic Developmental Temperatures Modulate Thermal Acclimation of Performance Curves in Tadpoles of the Frog Limnodynastes peronii

Seebacher

Grigaltchik

2014

PLoS ONE

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“…From a physiological perspective, there is a direct effect of temperature on the biochemical reactions involved in locomotion. For example, high temperatures enable increased speed of muscle contractions and muscle energy efficiency; cockroaches can achieve higher rates of O 2 consumption and running speeds (Full and Tullis 1990) and frog muscle requires less O 2 per unit work rate due to reduced viscosity and stiffness (Seebacher et al 2014). Co-adaptation between behaviour and physiology would interconnect these factors, with animals expected to select temperature ranges that maximise physiological performance, e.g.…”

Section: Discussionmentioning

confidence: 99%

The interactions between temperature and activity levels in driving metabolic rate: theory, with empirical validation from contrasting ectotherms

et al. 2015

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The rate of change in resting metabolic rate (RMR) as a result of a temperature increase of 10 °C is termed the temperature coefficient (Q10), which is often used to predict how an organism's total MR will change with temperature. However, this method neglects a potentially key component of MR; changes in activity level (and thus activity MR; AMR) with temperature may significantly alter the relationship between MR and temperature. The present study seeks to describe how thermal effects on total MR estimated from RMR-temperature measurements can be misleading when the contribution of activity to total MR is neglected. A simple conceptual framework illustrates that since the relationship between activity levels and temperature can be different to the relationship between RMR and temperature, a consistent relationship between RMR and total MR cannot be assumed. Thus the thermal effect on total MR can be considerably different to the thermal effect on RMR. Simultaneously measured MR and activity from three ectotherm species with differing behavioural and physiological ecologies were used to empirically examine how changes in temperature drive changes in RMR, activity level, AMR and the Q10 of MR. These species exhibited varied activity- and MR-temperature relationships, underlining the difficulty in predicting thermal influences on activity levels and total MR. These data support a model showing that thermal effects on total MR will deviate from predictions based solely on RMR; this deviation will depend upon the difference in Q10 between AMR and RMR, and the relative contribution of AMR to total MR. To develop mechanistic, predictive models for species' metabolic responses to temperature changes, empirical information about the relationships between activity levels, MR and temperature, such as reported here, is required. This will supersede predictions based on RMR alone.

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“…When respirometry techniques are applied in ecological research, logistical challenges arise associated with the desire to carry out experiments in field locations or conduct studies which require large sample sizes. In this type of research, there is an increasing trend towards using static respirometry techniques without the inclusion of a mixing device (Donelson et al , 2011 a ; Donelson and Munday, ; Miller et al , ; Handelsman et al , ; Seebacher et al , ; Seebacher et al , 2014 a , b ). It is important to understand how the exclusion of a mixing device will affect the results of a study.…”

Section: Introductionmentioning

confidence: 99%

Experimental methods in aquatic respirometry: the importance of mixing devices and accounting for background respiration

Rodgers

Tenzing

Clark

2016

Journal of Fish Biology

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In light of an increasing trend in fish biology towards using static respirometry techniques without the inclusion of a mixing mechanism and without accurately accounting for the influence of microbial (background) respiration, this paper quantifies the effect of these approaches on the oxygen consumption rates (ṀO2 ) measured from juvenile barramundi Lates calcarifer (mean ± s.e. mass = 20·31 ± 0·81 g) and adult spiny chromis damselfish Acanthochromis polyacanthus (22·03 ± 2·53 g). Background respiration changed consistently and in a sigmoidal manner over time in the treatment with a mixing device (inline recirculation pump), whereas attempts to measure background respiration in the non-mixed treatment yielded highly variable estimates of ṀO2 that were probably artefacts due to the lack of water movement over the oxygen sensor during measurement periods. This had clear consequences when accounting for background respiration in the calculations of fish ṀO2 . Exclusion of a mixing device caused a significantly lower estimate of ṀO2 in both species and reduced the capacity to detect differences between individuals as well as differences within an individual over time. There was evidence to suggest that the magnitude of these effects was dependent on the spontaneous activity levels of the fish, as the difference between mixed and non-mixed treatments was more pronounced for L. calcarifer (sedentary) than for A. polyacanthus (more spontaneously active). It is clear that respirometry set-ups for sedentary species must contain a mixing device to prevent oxygen stratification inside the respirometer. While more active species may provide a higher level of water mixing during respirometry measurements and theoretically reduce the need for a mixing device, the level of mixing cannot be quantified and may change with diurnal cycles in activity. To ensure consistency across studies without relying on fish activity levels, and to enable accurate assessments of background respiration, it is recommended that all respirometry systems should include an appropriate mixing device.

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The cost of muscle power production: muscle oxygen consumption per unit work increases at low temperatures inXenopus laevisDaudin

Cited by 19 publications

References 50 publications

Embryonic Developmental Temperatures Modulate Thermal Acclimation of Performance Curves in Tadpoles of the Frog Limnodynastes peronii

Embryonic Developmental Temperatures Modulate Thermal Acclimation of Performance Curves in Tadpoles of the Frog Limnodynastes peronii

The interactions between temperature and activity levels in driving metabolic rate: theory, with empirical validation from contrasting ectotherms

Experimental methods in aquatic respirometry: the importance of mixing devices and accounting for background respiration

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