The ecological environment of the Wuling Mountains region has been impacted by climate change and economic development, necessitating immediate reinforcement of ecological protection and restoration measures. The study utilized the normalized vegetation index (NDVI) as a proxy for ecological resilience. NDVI data from 2000 to 2020 were employed to compute the ecological resilience index of the Wuling Mountains area and to examine its spatial and temporal evolution as well as the factors influencing it. The findings indicate that: (1) The ecological resilience index increased in the Wuling Mountains area and Guizhou, Chongqing, and Hunan sub-areas but decreased in the Hubei sub-area. (2) The ecological resilience varies significantly in the Wuling Mountains area and the Guizhou, Hubei, and Hunan sub-regions, whereas it varies less in the Chongqing sub-region. (3) The primary elements influencing the ecological resilience capability of the Wuling Mountains area and its four sub-areas are climate conditions and socio-economic factors, respectively. The study can offer a scientific foundation for ecological conservation and restoration efforts in the Wuling Mountains area, as well as serve as a benchmark for measuring ecological resilience in other environmentally vulnerable regions.