The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

Wipff, Pierre-Jean; Majd, Hicham; Acharya, Chitrangada; Buscemi, Lara; Meister, Jean-Jacques; Hinz, Boris

doi:10.1016/j.biomaterials.2008.12.022

Cited by 115 publications

(131 citation statements)

References 60 publications

Supporting

Mentioning

123

Contrasting

Order By: Relevance

“…11,33,43 However, the adsorption of these proteins on PDMS is very difficult to control and is instable over time. 19 All these studies led to a collective alignment of myotubes, with an angle that depended on the geometry of the micropattern (lines or square patterns). The depth of the pattern was also found to affect myotube alignment, 44,45 with a depth of 2-4 mm leading to a better alignment.…”

Section: Filmsmentioning

confidence: 99%

“…16 However, one of the major drawbacks of PDMS is its hydrophobicity, which renders cell cultures over long time periods difficult to maintain. Indeed, the surface modification of PDMS by the adsorption of ECM proteins, such as gelatin, 17 fibronectin, 18 or laminin, 9 by covalent grafting 19,20 or polyelectrolyte multilayer (PEM) coating, 21 is required for long-term cell adhesion. In the present study, we use PEM films as biomimetic matrices with tunable stiffness to modify the PDMS surface.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Engineering Muscle Tissues on Microstructured Polyelectrolyte Multilayer Films

Monge

Ren

Berton

et al. 2012

Tissue Engineering Part A

View full text Add to dashboard Cite

Section: Filmsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineering Muscle Tissues on Microstructured Polyelectrolyte Multilayer Films

Monge

Ren

Berton

et al. 2012

Tissue Engineering Part A

View full text Add to dashboard Cite

“…3a). In a series of preliminary experiments, we determined that silicone surface functionalization for covalent binding of protein and sNAG in consequent treatments with plasma oxygen, APTES, and glutaraildehyde [19,20] was pivotal for long-term stability of the coat (Fig. 1c).…”

Section: Production Of Stencils With Fa Microstructure and Protein Dementioning

confidence: 99%

“…To provide surfaces with a non-protein adhesive molecule, we used poly N-acetyl glucosamine (sNAG, Marine Polymer Technologies, Inc., Burlington, MA, USA). For complete coating, collagen, FN, and sNAG were covalently bound to silicone substrates, in consequent treatments with plasma oxygen, 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde [19,20] (Fig. 1c).…”

Section: Silicone Implants and Protein Depositionmentioning

confidence: 99%

Novel micropatterns mechanically control fibrotic reactions at the surface of silicone implants

et al. 2015

Self Cite

View full text Add to dashboard Cite

a b s t r a c tOver the past decade, various implantable devices have been developed to treat diseases that were previously difficult to manage such diabetes, chronic pain, and neurodegenerative disorders. However, translation of these novel technologies into clinical practice is often difficult because fibrotic encapsulation and/or rejection impairs device function after body implantation. Ideally, cells of the host tissue should perceive the surface of the implant being similar to the normal extracellular matrix. Here, we developed an innovative approach to provide implant surfaces with adhesive protein micropatterns. The patterns were designed to promote adhesion of fibroblasts and macrophages by simultaneously suppressing fibrogenic activation of both cell types. In a rat model, subcutaneously implanted silicone pads provided with the novel micropatterns caused 6-fold lower formation of inflammatory giant cells compared with clinical grade, uncoated, or collagen-coated silicone implants. We further show that micropatterning of implants resulted in 2e3-fold reduced numbers of pro-fibrotic myofibroblast by inhibiting their mechanical activation. Our novel approach allows controlled cell attachment to implant surfaces, representing a critical advance for enhanced biointegration of implantable medical devices.

show abstract

“…In the 2D stretching system, using PDMS as a culture film limits biological investigation to short-term experiments, as cell adhesion to the substrate begins to be substantially affected after 6 hours of stimulation. This can be rectified by covalently binding matrix proteins to the PDMS surface 11 , or by replacing the PDMS cell culture substrate with a more clinically relevant polyurethane material, to improve cell adhesion and provide greater flexibility in conducted biological experiments 12 . In the described 3D system, the hydrogel chemistry is intended as an illustrative example only.…”

Section: Discussionmentioning

confidence: 99%

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Moraes

Sun

Simmons

2010

JoVE

View full text Add to dashboard Cite

The ability to systematically probe in vitro cellular response to combinations of mechanobiological stimuli for tissue engineering, drug discovery or fundamental cell biology studies is limited by current bioreactor technologies, which cannot simultaneously apply a variety of mechanical stimuli to cultured cells. In order to address this issue, we have developed a series of microfabricated platforms designed to screen for the effects of mechanical stimuli in a high-throughput format. In this protocol, we demonstrate the fabrication of a microactuator array of vertically displaced posts on which the technology is based, and further demonstrate how this base technology can be modified to conduct high-throughput mechanically dynamic cell culture in both two-dimensional and three-dimensional culture paradigms. Protocol A. Device description and operationDevices are fabricated using multilayer soft lithography 1 in polydimethylsiloxane (PDMS), and are capable of simultaneously generating a range of mechanical conditions in individual cell culture locations across the microfabricated array. In this protocol, the steps to fabricate an array of pneumatically-actuated microposts are first described, followed by steps to modify the device to enable mechanically dynamic culture in both two-dimensional (2D) and three-dimensional (3D) culture paradigms. The outlined microfabricated approach increases throughput over existing macroscale systems, and is best suited for screening for the effects of a variety of mechanical conditions. The operational principle for the device is based on an array of vertically actuated microposts. Microposts are fabricated on a freely-suspended diaphragm, and are raised and lowered by applying positive and negative pressures beneath the actuation diaphragm (Figure 1). A key feature of the array is that by varying the size of the actuation diaphragm, a single pressure source can be used to obtain a range of vertical displacements across the array. This principle is used to rapidly screen cellular response to a large number of mechanical stimulatory conditions across a single device.Based on an analogous macroscale design by Schaffer et al. 2 , our design includes a second suspended and lubricated cell culture film over the post, allowing cells to experience 2D substrate deformation as the culture film slips over the raised loading post. Alternatively, photopatterning an array of cell-laden hydrogels over the loading posts allows for compressive stimulation of cells in 3D culture. Detailed instructions in setting up these systems follow. B. Fabrication of the pneumatic microactuator arrayFabricating the pneumatic microactuator array requires stringent alignment in multilayer PDMS structures. This is challenging, due to shrinkage-induced alignment registration errors. To address this, we use a fabrication process termed 'sandwich mold fabrication' 3 , shown to effectively eliminate this issue 4 . Materials Preparation1. Single-or multi-level SU-8 masters for each of the various levels are f...

show abstract

The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

Cited by 115 publications

References 60 publications

Engineering Muscle Tissues on Microstructured Polyelectrolyte Multilayer Films

Engineering Muscle Tissues on Microstructured Polyelectrolyte Multilayer Films

Novel micropatterns mechanically control fibrotic reactions at the surface of silicone implants

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Contact Info

Product

Resources

About