The CRC orthologue from Pisum sativum shows conserved functions in carpel morphogenesis and vascular development

Fourquin, Chloé; Primo-Capella, Amparo; Martínez-Fernández, Irene; Huet-Trujillo, Estefanía; Ferrándiz, Cristina

doi:10.1093/aob/mcu129

Cited by 38 publications

(43 citation statements)

References 37 publications

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“…It also indicated that DL interacts antagonistically with class B genes and controls floral meristem determinacy (Yamaguchi et al , 2004). Ancestral expression patterns and functional studies performed with Petunia hybrida , Nicotiana tabacum and Pisum sativum support an ancestral role of DL/CRC genes in the specification of carpel development and floral meristem termination (Lee et al , 2005; Yamada et al , 2011; Fourquin et al , 2014). Interestingly, in basal eudicot California poppy, EcCRC not only harbors ancestral functions of DL/CRC but is also involved in ovule initiation (Orashakova et al , 2009).…”

Section: Introductionmentioning

confidence: 80%

“…The expression patterns of eudicot DL / CRC genes are consistent with their functions, including termination of the floral meristem, promotion of gynoecium growth, and elaboration of the abaxial carpel wall structures (Yamada et al , 2011). The legumes’ PsCRC is not only significantly expressed in carpels through all of the floral buds, but also in the ovary chamber, style–stigma junction, stigmatic tissues, and ovary wall (Fourquin et al , 2014). Some of the core eudicots have recruited DL / CRC -like genes for nectary development (Lee et al , 2005).…”

Section: Discussionmentioning

confidence: 99%

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Ancestral duplicatedDL/CRCorthologs display function on orchid reproductive organ innovation

Chen

Hsiao

et al. 2020

Preprint

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27The orchid flower is renowned for complexity of flower organ morphogenesis and has 28 attracted great interest from scientists. The YABBY genes encode plant-specific transcription 29 factors with important roles in vegetative and reproductive development in seed plants. 30 DROOPING LEAF/CRABS CLAW (DL/CRC) orthologs are involved in reproductive organ 31 development (especially carpels) of angiosperms. Orchid gynostemium (the fused organ of 32 the androecium and gynoecium) and ovule development are unique developmental processes. 33 Understanding the DL/CRC-like genes controlling the developmental program of the 34 gynostemium and ovule could provide accessible information for reproductive organ 35 molecular regulation in orchids. Two DL/CRC-like genes, named PeDL1 and PeDL2, were 36 cloned from Phalaenopsis equestris. The orchid DL/CRC forms a monophyletic clade with 37 two subclades including AshDL, PeDL1 and DcaDL1 in subclade I, and PeDL2 and DcaDL2 38 in subclade II. The temporal and spatial expression analysis indicated PeDL genes are 39 specifically expressed in the gynostemium and at the early stages of ovule development. Both 40 PeDLs could partially complement an Arabidopsis crc-1 mutant. Transient overexpression of 41 PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity 42 of gynostemium. PeDL1, instead of PeDL2, could form a heterodimer with PeCIN8. 43 Paralogue retention and subsequent divergence of the gene sequence of PeDL1 and PeDL2 in 44 P. equestris might result in the differentiation of function and protein behaviors. These results 45 reveal the important roles of PeDLs involved in orchid gynostemium and ovule development 46 and provide new insights for further understanding the molecular mechanisms underlying 47 orchid reproductive organ development. 48 49 52 Over the last couple of decades, genes involved in angiosperm floral organ specification 68 were identified and the 'ABCDE model' was proposed, with the combined effect of the A-, B-, 69 C-, D-, and E-class MADS-box genes determining the floral organ identity. In monocot 70 orchids, the Phalaenopsis species was used as a model plant to delineate the B-, C-, D-, and 71 E-class functions of MADS-box genes participating in specialized floral organ development 72 (Pan et al., 2014; Tsai et al., 2014). The most notable feature is that P. equestris contains four 73 B-class AP3-like genes with differential expression largely responsible for differentiation of 74 the two closely-spaced tripartite perianth whorls into three sepals of the outer whorl, versus 75 the inner whorl of two lateral petals and a median labellum (Tsai et al., 2004). Later, the 76 refined 'orchid code' and 'Homeotic Orchid Tepal (HOT)' models were, respectively, 77proposed to illustrate the regulation of perianth morphogenesis in orchids 78 4 (Mondragon- Palomino & Theissen, 2011; Pan et al., 2011). The studies of floral terata from 79Phalaenopsis and Cymbidium agreed that C-and D-class MADS-box genes correlated with 80 the gynostemium and...

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Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Ancestral duplicatedDL/CRCorthologs display function on orchid reproductive organ innovation

Chen

Hsiao

et al. 2020

Preprint

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“…Therefore, it is likely that in Juncus , DL expression in vascular bundles did not contribute to the thickening growth but might play a role in vascular bundle differentiation, particularly of sclerenchyma cells at the xylem pole. In addition, the initial ring‐like expression surrounding provascular strands is similar to maize (Strable et al ., ) and garden pea (Fourquin et al ., ). Our result supports the hypothesis that the function of CRC / DL in vascular development is ancestral rather than derived (Fourquin et al ., ), although its different expression patterns within vascular bundles of Juncus (this study; Yamaguchi et al ., , ), maize (Strable et al ., ), A. asparagoides (Nakayama et al ., ), and garden pea (Fourquin et al ., ) may suggest further functional diversification along different evolutionary trajectories.…”

Section: Discussionmentioning

confidence: 97%

Morphogenesis of flattened unifacial leaves in Juncus prismatocarpus (Juncaceae)

Yin

Tsukaya

2019

New Phytologist

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Summary To reveal the mode of morphogenesis of flattened unifacial leaves, we analysed the cell division direction and distribution on the leaf blade of Juncus prismatocarpus. Using the pulse–chase 5‐ethynyl‐2′‐deoxyuridine method, we quantified and mapped the cell division direction on the leaf blade of J. prismatocarpus and compared the distribution of thickening cell divisions with the expression pattern of DROOPING LEAF (DL), a key gene involved in leaf blade thickening. Thickening cell divisions were the most abundant (> 45%) among all cell division directions on the leaf blade of J. prismatocarpus from the early plastochron 2 stage through the plastochron 3 stage. Mapping of cell divisions indicated that cell divisions in a particular direction were not restricted to a particular domain but were distributed diffusely throughout the entire cross‐sectional area of the leaf blade. Gradient analysis indicated that the distribution of thickening cell divisions of the adaxial domain was denser than that of the abaxial domain. Contrary to the prolonged and diffuse distribution of thickening cell divisions, DL expression was transient and restricted in a narrow band. Our results suggest that a diffuse ‘thickening meristem’ plays the key role in the development of flattened unifacial leaves.

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“…CRC and INO were uniformly expressed only in the reproductive developmental stages of A. thaliana, B. rapa, and B. sinuspersici, suggesting their conserved function in carpel morphogenesis, gynoecium differentiation, floral meristem, and outer integument development [21,40]. High BsCRC expression during the pre-anthesis elongation stage was detected.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of the YABBY Gene Family of Bienertia sinuspersici, a Single-Cell C4 Plant

Soundararajan

Won

Park

et al. 2019

Plants

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The emergence and expression of the YABBY gene family (YGF) coincided with the evolution of leaves in seed plants, and was integral to the early evidence of lamina followed by reproductive development. YGF contains six subclasses, i.e., CRC, INO, FIL, YAB2, YAB3, and YAB5. This study aims to extract the genome sequences of the YGF in Bienertia sinuspersici, an important model plant for single-cell C4 (SCC4), non-Kranz photosynthesis. A comparative genomic analysis was undertaken with Vitis vinefera, Arabidopsis thaliana, Brassica rapa, and Chenopodium quinoa. Six copies of YGF were present in B. sinuspersici and A. thaliana with a single copy of each YGF subgroup. V. vinefera possessed seven copies of YGF with duplicates in FIL and YAB2 subgroups, but no YAB3. B. rapa and C. quinoa after whole genome duplication contained additional copies of YGF. The gene structure and conserved motifs were analyzed among the YGF. In addition, the relative quantification of YGF was analyzed in the leaves, reproductive developmental stages such as the bud, and the pre-anthesis and anthesis stages in B. sinuspersici, A. thaliana, and B. rapa. CRC and INO possessed conserved floral-specific expression. Temporal and perpetual changes in the expression of YGF orthologs were observed in the leaves and reproductive developmental stages. The results of this study provide an overview of YGF evolution, copy number, and its differential expression in B. sinuspersici. Further studies are required to shed light on the roles of YABBY genes in the evolution of SCC4 plants and their distinct physiologies.

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The CRC orthologue from Pisum sativum shows conserved functions in carpel morphogenesis and vascular development

Cited by 38 publications

References 37 publications

Ancestral duplicatedDL/CRCorthologs display function on orchid reproductive organ innovation

Ancestral duplicatedDL/CRCorthologs display function on orchid reproductive organ innovation

Morphogenesis of flattened unifacial leaves in Juncus prismatocarpus (Juncaceae)

Comparative Analysis of the YABBY Gene Family of Bienertia sinuspersici, a Single-Cell C4 Plant

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