The Critical Role of Cell Homing in Cytotherapeutics and Regenerative Medicine

He, Xiao-Tao; Wang, Jia; Li, Xuan; Yin, Yuan; Sun, Hao; Chen, Fa‐Ming

doi:10.1002/adtp.201800098

Cited by 11 publications

(6 citation statements)

References 211 publications

(197 reference statements)

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“…Endeavors aiming to bridge the gap between the promising results obtained with animal models and the reality of clinical treatments indicate that clinical success can be facilitated by avoiding the use of external stem cells. Therefore, studies have established endogenous regenerative techniques involving material design and/or cytokine presentation that permit the patient's own cells to be harnessed for therapeutics . An evolving body of work in this topic corroborates that the recreation of a suitable microenvironment at the injured site can instruct the homing of resident stem cells and can induce the periodontium itself to regenerate.…”

Section: Resultsmentioning

confidence: 99%

Concise Review: Periodontal Tissue Regeneration Using Stem Cells: Strategies and Translational Considerations

Wang

et al. 2018

Stem Cells Translational Medicine

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Periodontitis is a widespread disease characterized by inflammation‐induced progressive damage to the tooth‐supporting structures until tooth loss occurs. The regeneration of lost/damaged support tissue in the periodontium, including the alveolar bone, periodontal ligament, and cementum, is an ambitious purpose of periodontal regenerative therapy and might effectively reduce periodontitis‐caused tooth loss. The use of stem cells for periodontal regeneration is a hot field in translational research and an emerging potential treatment for periodontitis. This concise review summarizes the regenerative approaches using either culture‐expanded or host‐mobilized stem cells that are currently being investigated in the laboratory and with preclinical models for periodontal tissue regeneration and highlights the most recent evidence supporting their translational potential toward a widespread use in the clinic for combating highly prevalent periodontal disease. We conclude that in addition to in vitro cell‐biomaterial design and transplantation, the engineering of biomaterial devices to encourage the innate regenerative capabilities of the periodontium warrants further investigation. In comparison to cell‐based therapies, the use of biomaterials is comparatively simple and sufficiently reliable to support high levels of endogenous tissue regeneration. Thus, endogenous regenerative technology is a more economical and effective as well as safer method for the treatment of clinical patients. Stem Cells Translational Medicine 2019;8:392–403

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Section: Resultsmentioning

confidence: 99%

Concise Review: Periodontal Tissue Regeneration Using Stem Cells: Strategies and Translational Considerations

Wang

et al. 2018

Stem Cells Translational Medicine

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159

122

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“…QNZ is an inhibitor of the NF-κB signaling pathway [21]. Bone regeneration can be divided into two stages: endogenous stem cell homing to the sites of bone defects and the subsequent osteogenic differentiation of the recruited stem cells [41,42]. In this project, we found that QNZ could rescue BMP2-mediated migration and osteogenic differentiation in MSCs inhibited by fibroblasts.…”

Section: Discussionmentioning

confidence: 85%

Synergistic Effect of QNZ, an Inhibitor of NF-κB Signaling, and Bone Morphogenetic Protein 2 on Osteogenic Differentiation in Mesenchymal Stem Cells through Fibroblast-Induced Yes-Associated Protein Activation

Huang

Wang

Zhang

et al. 2023

IJMS

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Biomaterials carrying recombinant human bone morphogenetic protein 2 (BMP2) have been developed to enhance bone regeneration in the treatment of bone defects. However, various reports have shown that in the bone repair microenvironment, fibroblasts can inhibit BMP2-induced osteogenic differentiation in mesenchymal stem cells (MSCs). Thus, factors that can target fibroblasts and improve BMP2-mediated osteogenesis should be explored. In this project, we focused on whether or not an inhibitor of the NF-κB signaling pathway, QNZ (EVP4593), could play a synergistic role with BMP2 in osteogenesis by regulating the activity of fibroblasts. The roles of QNZ in regulating the proliferation and migration of fibroblasts were examined. In addition, the effect of QNZ combined with BMP2 on the osteogenic differentiation of MSCs was evaluated both in vitro and in vivo. Furthermore, the detailed mechanisms by which QNZ improved BMP2-mediated osteogenesis through the modulation of fibroblasts were analyzed and revealed. Interestingly, we found that QNZ inhibited the proliferation and migration of fibroblasts. Thus, QNZ could relieve the inhibitory effects of fibroblasts on the homing and osteogenic differentiation of mesenchymal stem cells. Furthermore, biomaterials carrying both QNZ and BMP2 showed better osteoinductivity than did those carrying BMP2 alone both in vitro and in vivo. It was found that the mechanism of QNZ involved reactivating YAP activity in mesenchymal stem cells, which was inhibited by fibroblasts. Taken together, our results suggest that QNZ may be a candidate factor for assisting BMP2 in inducing osteogenesis. The combined application of QNZ and BMP2 in biomaterials may be promising for the treatment of bone defects in the future.

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“…Cellular homing is the concept of the tight integration of cells to the native tissue through intercellular adhesion [ 31 ]. There were conflicting findings in the ability of MSCs to attach to the affected tissue.…”

Section: Discussionmentioning

confidence: 99%

Type II Collagen-Conjugated Mesenchymal Stem Cells Micromass for Articular Tissue Targeting

et al. 2021

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The tissue engineering approach in osteoarthritic cell therapy often requires the delivery of a substantially high cell number due to the low engraftment efficiency as a result of low affinity binding of implanted cells to the targeted tissue. A modification towards the cell membrane that provides specific epitope for antibody binding to a target tissue may be a plausible solution to increase engraftment. In this study, we intercalated palmitated protein G (PPG) with mesenchymal stem cells (MSCs) and antibody, and evaluated their effects on the properties of MSCs either in monolayer state or in a 3D culture state (gelatin microsphere, GM). Bone marrow MSCs were intercalated with PPG (PPG-MSCs), followed by coating with type II collagen antibody (PPG-MSC-Ab). The effect of PPG and antibody conjugation on the MSC proliferation and multilineage differentiation capabilities both in monolayer and GM cultures was evaluated. PPG did not affect MSC proliferation and differentiation either in monolayer or 3D culture. The PPG-MSCs were successfully conjugated with the type II collagen antibody. Both PPG-MSCs with and without antibody conjugation did not alter MSC proliferation, stemness, and the collagen, aggrecan, and sGAG expression profiles. Assessment of the osteochondral defect explant revealed that the PPG-MSC-Ab micromass was able to attach within 48 h onto the osteochondral surface. Antibody-conjugated MSCs in GM culture is a potential method for targeted delivery of MSCs in future therapy of cartilage defects and osteoarthritis.

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The Critical Role of Cell Homing in Cytotherapeutics and Regenerative Medicine

Cited by 11 publications

References 211 publications

Concise Review: Periodontal Tissue Regeneration Using Stem Cells: Strategies and Translational Considerations

Concise Review: Periodontal Tissue Regeneration Using Stem Cells: Strategies and Translational Considerations

Synergistic Effect of QNZ, an Inhibitor of NF-κB Signaling, and Bone Morphogenetic Protein 2 on Osteogenic Differentiation in Mesenchymal Stem Cells through Fibroblast-Induced Yes-Associated Protein Activation

Type II Collagen-Conjugated Mesenchymal Stem Cells Micromass for Articular Tissue Targeting

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