The Critical Roles of Progesterone Receptor (PGR) in Ovulation, Oocyte Developmental Competence and Oviductal Transport in Mammalian Reproduction

Akison, Lisa K.; Robker, Rebecca L.

doi:10.1111/j.1439-0531.2012.02088.x

Cited by 74 publications

(53 citation statements)

References 108 publications

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“…This influx is postulated to occur in part through LH mediated pathways that increase vasodilation and vascular permeability, allowing the transmigration of leukocytes from the blood vessels to the preovulatory follicle (25). Mice lacking genes that are responsible for the LH mediated inflammatory reaction, such as the PGR (26,27), PTGS2 (28), or increased vasodilation, such as endothelin 2 (29), fail to ovulate or have reduced ovulatory capacity (30). However, the importance of leukocytes in the ovulatory process is evident from observations that the number of oocytes released increased approximately 3-fold when leukocytes were added to perfused rat ovaries (4), whereas depletion of leukocytes from the blood decreased the number of ovulations (3,5).…”

Section: Discussionmentioning

confidence: 98%

Chemokine Ligand 20: A Signal for Leukocyte Recruitment During Human Ovulation?

et al. 2015

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Ovulation is one of the cornerstones of female fertility. Disruption of the ovulatory process results in infertility, which affects approximately 10% of couples. Using a unique model in which the dominant follicle is collected across the periovulatory period in women, we have identified a leukocyte chemoattractant, chemokine ligand 20 (CCL20), in the human ovary. CCL20 mRNA is massively induced after an in vivo human chorionic gonadotropin (hCG) stimulus in granulosa (>10 000-fold) and theca (>4000-fold) cells collected during the early ovulatory (12-18 h) and late ovulatory (18-34 h) periods after hCG administration. Because the LH surge sets in motion an inflammatory reaction characterized by an influx of leukocytes and CCL20 is known to recruit leukocytes in other systems, the composition of ovarian leukocytes (CD45+) containing the CCL20 receptor CCR6 was determined immediately prior to ovulation. CD45+/CCR6+ cells were primarily natural killer cells (41%) along with B cells (12%), T cells (11%), neutrophils (10%), and monocytes (9%). Importantly, exogenous CCL20 stimulated ovarian leukocyte migration 59% within 90 minutes. Due to the difficulties in obtaining human follicles, an in vitro model was developed using granulosa-lutein cells to explore CCL20 regulation. CCL20 expression increased 40-fold within 6 hours after hCG, was regulated partially by the epithelial growth factor pathway, and was positively correlated with progesterone production. These results demonstrate that hCG dramatically increases CCL20 expression in the human ovary, that ovarian leukocytes contain the CCL20 receptor, and that CCL20 stimulates leukocyte migration. Our findings raise the prospect that CCL20 may aid in the final ovulatory events and contribute to fertility in women.

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Section: Discussionmentioning

confidence: 98%

Chemokine Ligand 20: A Signal for Leukocyte Recruitment During Human Ovulation?

et al. 2015

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“…Steroid hormones play major roles in reproduction (Mulac-Jericevic & Conneely, 2004;Akison & Robker, 2012), notably during gestation (Simpson & MacDonald, 1981). For instance the elevation of progesterone plasma concentration is essential for the establishment and maintenance of pregnancy (Soûles, 1989;Baird et al, 1997;Magiakou et al, 1997;Ferin, 1999;Ark, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of a brief stress on progesterone plasma levels in pregnant and non-pregnant guinea pigs

Michel

Bonnet

2014

Animal Biol

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International audienceSteroid hormones are essential for vertebrate reproduction. They are involved in the regulation of major female reproductive events like ovulation, embryo implantation, or gestation. For instance, progesterone promotes foeto-maternal exchanges whereas glucocorticoids stimulate the mobilization of the required energy resources. However, glucocorticoids are key effectors of the stress response; chronic elevations of these hormones can exert negative effects on reproduction. Yet, little is known about the effects of a brief exposure to a stressor on the respective plasma concentrations of sex steroids and glucocorticoids, notably during pregnancy. We examined the impact of a brief stress (handling + 1.5 hours in a bag) on progesterone and cortisol plasma concentrations in pregnant and non-pregnant female guinea pigs. Analyses revealed that: 1) pregnant females exhibited higher baseline progesterone and cortisol concentrations compared to non-pregnant females, as expected; 2) cortisol concentrations increased rapidly following manipulation, revealing a typical hormonal stress response; and, 3) progesterone concentrations decreased on average by 50.9% following the brief stress period, both in pregnant and non-pregnant individuals. These experimental results show for the first time a drastic and rapid impact on progesterone concentration caused by a brief stress

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“…Studies in mice and in human have demonstrated the critical role of nPR in ovulation910. Female mice lacking nPR exhibit anovulation, impaired sexual behavior, uterine dysfunction, impaired ductal branching morphogenesis and lobuloalveolar differentiation of the mammary gland11121314.…”

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confidence: 99%

Gene knockout of nuclear progesterone receptor provides insights into the regulation of ovulation by LH signaling in zebrafish

Tang

Liu

et al. 2016

Sci Rep

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It is well established that the luteinizing hormone surge triggers ovulation, a dynamic process leading to the release of the mature oocyte from the ovarian follicle. But how this process controlled by LH signaling remains largely unknown in non-mammalian species. In this study, we investigated the roles of nuclear progesterone receptor (npr) in LH-induced ovulation. Our results indicate that the nuclear progesterone receptor serves as an important mediator of LH action on ovulation. This conclusion is based on the following results: (1) the expression level of npr peaks at the full-grown stage of the follicles; (2) the expression of npr is stimulated by LH signaling in vitro and in vivo; and (3) the npr null females are infertile due to ovulation defects. Moreover, we further show that LH signaling could induce ptger4b expression in an npr-dependent manner, and blockage of Ptger4b could also block hCG-induced ovulation. Collectively, our results not only demonstrate that npr serves an indispensable role in mediating the action of LH on ovulation in zebrafish, but also provide insights into the molecular mechanisms of the regulation of ovulation in fish.

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The Critical Roles of Progesterone Receptor (PGR) in Ovulation, Oocyte Developmental Competence and Oviductal Transport in Mammalian Reproduction

Cited by 74 publications

References 108 publications

Chemokine Ligand 20: A Signal for Leukocyte Recruitment During Human Ovulation?

Chemokine Ligand 20: A Signal for Leukocyte Recruitment During Human Ovulation?

Effect of a brief stress on progesterone plasma levels in pregnant and non-pregnant guinea pigs

Gene knockout of nuclear progesterone receptor provides insights into the regulation of ovulation by LH signaling in zebrafish

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