The critical‐size supraalveolar peri‐implant defect model: reproducibility in histometric data acquisition of alveolar bone formation and osseointegration

Lee, Jae-Bum; Tran, Quoc Van; Seeba, Gary; Wikesjö, Ulf M.E.; Susin, Cristiano

doi:10.1111/j.1600-051x.2009.01487.x

Cited by 10 publications

(10 citation statements)

References 41 publications

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“…1,2 Although alveolar bones have the capability to regenerate, the healing process frequently fails to progress through the normal stages of healing or is delayed when the defects reach a critical size. [3][4][5] Therefore, bone grafting is necessary for large defects.…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Du¹,

Liu²,

Deng³

et al. 2015

IJN

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To improve the regenerative performance of nano-hydroxyapatite/coralline (nHA/coral) block grafting in a canine mandibular critical-size defect model, nHA/coral blocks were coated with recombinant human vascular endothelial growth factor 165 (rhVEGF) via physical adsorption (3 μg rhVEGF 165 per nHA/coral block). After the nHA/coral blocks and VEGF/nHA/coral blocks were randomly implanted into the mandibular box-shaped defects in a split-mouth design, the healing process was evaluated by histological observation and histomorphometric and immunohistological analyses. The histological evaluations revealed the ingrowth of newly formed blood vessels and bone at the periphery and cores of the blocks in both groups at both 3 and 8 weeks postsurgery, respectively. In the histomorphometric analysis, the VEGF/nHA/coral group exhibited a larger quantity of new bone formation at 3 and 8 weeks postsurgery. The percentages of newly formed bone within the entire blocks in the VEGF/nHA/coral group were 27.3%±8.1% and 39.3%±12.8% at 3 weeks and 8 weeks, respectively, and these values were slightly greater than those of the nHA/coral group (21.7%±3.0% and 32.6%±10.3%, respectively), but the differences were not significant ( P >0.05). The immunohistological evaluations revealed that the neovascular density in the VEGF/nHA/coral group (146±32.9 vessel/mm 2 ) was much greater than that in the nHA/coral group (105±51.8 vessel/mm 2 ) at the 3-week time point ( P <0.05), but no significant difference was observed at the 8-week time point (341±86.1 and 269±50.7 vessel/mm 2 , respectively, P >0.05). The present study indicated that nHA/coral blocks might be optimal scaffolds for block grafting in critical-size mandibular defects and that additional VEGF coating via physical adsorption can promote angiogenesis in the early stage of bone healing, which suggests that prevascularized nHA/coral blocks have significant potential as a bioactive material for bone regeneration in large-scale alveolar defects.

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Section: Introductionmentioning

confidence: 99%

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Du¹,

Liu²,

Deng³

et al. 2015

IJN

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“…Alveolar bone augmentation represents a substantial component of clinical practices aimed at promoting bone formation to fill defects caused by periodontal destruction, tumor excision, or trauma [27,28]. In clinical practice, labial bone defects are a critical concern and difficult to restore.…”

Section: Discussionmentioning

confidence: 99%

“…A mid-crestal incision was made, and full-thickness mucoperiosteal flaps were raised. Rectangular saddle-like through-and-through defects were surgically created using a mini-surgical saw (Figure 2b,c) and abundant irrigation with sterile saline [27][28][29]. Meticulous efforts were expended to standardize the size of the saddle defects (depth: 4 mm; width: 10 mm; Figure 2f).…”

Section: Defect Creation Dental Implant Insertion and Bone Graft Inmentioning

confidence: 99%

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Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects

et al. 2020

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The aim of this study was to evaluate the efficacy of bone regeneration in developed bioceramics composed of dicalcium phosphate and hydroxyapatite (DCP/HA). Critical bony defects were prepared in mandibles of beagles. Defects were grafted using DCP/HA or collagen-enhanced particulate biphasic calcium phosphate (TCP/HA/Col), in addition to a control group without grafting. To assess the efficacy of new bone formation, implant stability quotient (ISQ) values, serial bone labeling, and radiographic and histological percentage of marginal bone coverage (PMBC) were carefully evaluated four, eight, and 12 weeks after surgery. Statistically significant differences among the groups were observed in the histological PMBC after four weeks. The DCP/HA group consistently exhibited significantly higher ISQ values and radiographic and histological PMCB eight and 12 weeks after surgery. At 12 weeks, the histological PMBC of DCP/HA (72.25% ± 2.99%) was higher than that in the TCP/HA/Col (62.61% ± 1.52%) and control groups (30.64% ± 2.57%). After rigorously evaluating the healing of biphasic DCP/HA bioceramics with a critical size peri-implant model with serial bone labeling, we confirmed that neutralized bioceramics exhibiting optimal compression strength and biphasic properties show promising efficacy in fast bone formation and high marginal bone coverage in peri-implant bone defects.

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“…[4748495051] Various studies in chronological order, listing the various types of animal models used in periodontal research with their respective research topic, are enumerated in Table 2. [5253545556]…”

Section: Various Animal Models Usedmentioning

confidence: 99%

Ethical guidelines, animal profile, various animal models used in periodontal research with alternatives and future perspectives

Pasupuleti¹,

Shetty²,

Salwaji³

2016

J Indian Soc Periodontol

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Laboratory animal models serve as a facilitator to investigate the etiopathogenesis of periodontal disease, are used to know the efficacy of reconstructive and regenerative procedures, and are also helpful in evaluation of newer therapeutic techniques including laser and implant therapies prior to application in the human beings. The aim of this review is to know the different animal models used in various specialties of dental research and to know the ethical guidelines prior to the usage of experimental models with main emphasis on how to refine, replace, and reduce the number of animal models usage in the laboratory. An online search for experimental animal models used in dental research was performed using MEDLINE/PubMed database. Publications from 2009 to May 2013 in the specialty of periodontics were included in writing this review. A total of 652 references were published in PubMed/MEDLINE databases based on the search terms used. Out of 245 studies, 241 were related to the periodontal research published in English from 2009 to 2013. Relevant papers were chosen according to the inclusion and exclusion criteria. After extensive electronic and hand search on animal models, it has been observed that various animal models were used in dental research. Search on animal models used for dental research purpose revealed that various animals such as rats, mice, guinea pigs, rabbit, beagle dogs, goats, and nonhuman primates were extensively used. However, with the new advancement of ex vivo animal models, it has become easy to investigate disease pathogenesis and to test the efficacy of newer therapeutic modalities with the reduced usage of animal models. This review summarized the large amount of literature on animal models used in periodontal research with main emphasis on ethical guidelines and on reducing the animal model usage in future perspective.

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The critical‐size supraalveolar peri‐implant defect model: reproducibility in histometric data acquisition of alveolar bone formation and osseointegration

Cited by 10 publications

References 41 publications

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects

Ethical guidelines, animal profile, various animal models used in periodontal research with alternatives and future perspectives

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