The crosstalk network of XIST/miR‐424‐5p/OGT mediates RAF1 glycosylation and participates in the progression of liver cancer

Deng, Ning; Jin, Cheqing; Du, Pengcheng; Liu, Qiumeng; Cheng, Qi; Li, Xue; Zhang, Bixiang; Chen, Xiaoping; Jiang, Li

doi:10.1111/liv.14904

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…Our results showed that XIST played essential roles in regulating cell migration and invasion, as silencing by siRNA significantly inhibited these functions in multiple HNC cells. These carcinogenic roles were also confirmed in previous cancer studies, such as liver ( 50 ), ovarian ( 51 ), and esophageal cancer ( 52 ). A review by Zhou et al.…”

Section: Discussionsupporting

confidence: 83%

Systematic Analysis and Identification of Dysregulated Panel lncRNAs Contributing to Poor Prognosis in Head-Neck Cancer

Tang

You²,

Chang³

et al. 2021

Front. Oncol.

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Head and neck cancer (HNC) is one of the most prevalent cancers worldwide, accounting for approximately 5% of all cancers. While the underlying molecules and their pathogenetic mechanisms in HNC have yet to be well elucidated, recent studies have shown that dysregulation of lncRNAs may disrupt the homeostasis of various biological pathways. However, the understanding of lncRNAs in HNC is still limited by the lack of expression profiling. In the present study, we employed a systematic strategy to identify a panel of lncRNA associated with HNC. A cancer-related lncRNA profile PCR array was screened to explore potential molecules specific for HNC. A total of 55 lncRNAs were found to be dysregulated in HNC cells when compared to normal keratinocytes. Further analysis of the prognostic significance using The Cancer Genome Atlas (TCGA) database revealed 15 lncRNAs highly correlated with overall survival in HNC patients. Additionally, clinical sample expression analysis of the TCGA-HNSC cohort revealed 16 highly dysregulated lncRNAs in HNC, resulting in a combined 31-lncRNA signature panel that could predict prognosis. Validation of these molecules confirmed the considerable level of altered expressions in HNC cells, with XIST, HOXA11-AS, TSIX, MALAT1, WT1-AS, and IPW being the most prominently dysregulated. We further selected a molecule from our panel (XIST) to confirm the validity of these lncRNAs in the regulation of cancer aggressiveness. Gene ontology (GO) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analyses demonstrated that XIST participated in various cancer-related functions, including cell proliferation and metastasis. XIST silencing with the RNAi technique substantially reduced invasion and migration in several HNC cell lines. Thus, our study defined a 31-lncRNA panel as prognostic signatures in HNC. These perspective results provide a knowledge foundation for further application of these molecules in precision medicine.

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Section: Discussionsupporting

confidence: 83%

Systematic Analysis and Identification of Dysregulated Panel lncRNAs Contributing to Poor Prognosis in Head-Neck Cancer

Tang

You²,

Chang³

et al. 2021

Front. Oncol.

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“…Hsa-mir-125b-5p was also found to be downregulated in bladder ( Canturk et al, 2014 ) and prostate cancer ( Lin et al, 2020 ). XIST, a lncRNA, has been shown to promote the progression of various cancers through its high expression ( Liu et al, 2018b ; Liu et al, 2019 ; Ning et al, 2021 ; Zheng et al, 2021 ). We found that the upregulated lncRNA XIST targets both hsa-mir-124-3p and hsa-miR-125b-5p and is positively correlated with CDKN2A.…”

Section: Discussionmentioning

confidence: 99%

The cuproptosis related genes signature predicts the prognosis and correlates with the immune status of clear cell renal cell carcinoma

Sun

Xu²,

Zhu

et al. 2022

Front. Genet.

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Background: Clear cell renal cell carcinoma (CCRCC) has a high incidence and poor prognosis. Cuproptosis, an independent pattern of cell death associated with copper, plays an important role in cancer proliferation and metastasis. The role of cuproptosis-related genes (CRGs) in CCRCC is unclear.Methods: Transcriptome and clinical information for CCRCC were downloaded from The Cancer Genome Atlas (TCGA) database. After dividing the training and testing cohort, a 4-CRGs risk signature (FDX1, DLD, DLAT, CDKN2A) was identified in the training cohort using Least absolute shrinkage and selection operator (LASSO) and Cox regression analysis. The effect of the 4-CRGs risk signature on prognosis was assessed using Kaplan-Meier (KM) curves and time-dependent receiver operating characteristic (ROC) curves and verified using the testing cohort. For different risk groups, the immune statue was assessed using the CIBERSORT algorithm, the ssGSEA method and immune checkpoint expression data. Finally, a competitive endogenous RNA (ceRNA) network was constructed using miRTarbase and starBase databases to identify molecules that may have a regulatory relationship with CRCCC.Results: There were significant changes in the overall survival (OS), immune microenvironment, immune function, and checkpoint gene expression among the different risk groups. A ceRNA network consisting of one mRNA, two miRNAs, and 12 lncRNAs was constructed.Conclusion: The 4-CRGs risk signature provides a new method to predict the prognosis of patients with CCRCC and the effect of immunotherapy. We propose a new cuproptosis-associated ceRNA network that can help to further explore the molecular mechanisms of CCRCC.

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“…A more detailed mechanism of XIST is demonstrated in hepatocellular carcinoma, in which inhibiting XIST expression contributes to overexpression of miR-424-5p. This miRNA degrades OGT and suppresses RAF1 glycosylation, resulting in favorable normal features in HCC cell lines [ 28 ].…”

Section: Cancer Cell Linesmentioning

confidence: 99%

Role of miR-424 in the carcinogenesis

et al. 2023

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Recent studies have revealed the impact of microRNAs (miRNAs) in the carcinogenic process. miR-424 is a miRNA whose role in this process is being to be identified. Experiments in the ovarian cancer, cervical cancer, hepatocellular carcinoma, neuroblastoma, breast cancer, osteosarcoma, intrahepatic cholangiocarcinoma, prostate cancer, endometrial cancer, non-small cell lung cancer, hemangioma and gastric cancer have reported down-regulation of miR-424. On the other hand, this miRNA has been found to be up-regulated in melanoma, laryngeal and esophageal squamous cell carcinomas, glioma, multiple myeloma and thyroid cancer. Expression of this miRNA is regulated by methylation status of its promoter. Besides, LINC00641, CCAT2, PVT1, LIN00657, LINC00511 and NNT-AS1 are among lncRNAs that act as molecular sponges for miR-424, thus regulating its expression. Moreover, several members of SNHG family of lncRNAs have been found to regulate expression of miR-424. This miRNA is also involved in the regulation of E2F transcription factors. The current review aims at summarization of the role of miR-424 in the process of cancer evolution and its impact on clinical outcome of patients in order to find appropriate markers for malignancies.

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The crosstalk network of XIST/miR‐424‐5p/OGT mediates RAF1 glycosylation and participates in the progression of liver cancer

Cited by 17 publications

References 33 publications

Systematic Analysis and Identification of Dysregulated Panel lncRNAs Contributing to Poor Prognosis in Head-Neck Cancer

Systematic Analysis and Identification of Dysregulated Panel lncRNAs Contributing to Poor Prognosis in Head-Neck Cancer

The cuproptosis related genes signature predicts the prognosis and correlates with the immune status of clear cell renal cell carcinoma

Role of miR-424 in the carcinogenesis

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