The crucial role of roots in increased cadmium-tolerance and Cd-accumulation in the pea mutant SGECd&lt;sup&gt;t&lt;/sup&gt;

Belimov, Andrey A.; Malkov, Nikita V.; Puhalsky, Jan V.; Tsyganov, V. E.; Bodyagina, K. B.; Safronova, Vera I.; Dietz, Karl‐Josef; Тихонович, И. А.

doi:10.1007/s10535-018-0789-0

Cited by 15 publications

(8 citation statements)

References 45 publications

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“…Although, at Cd2, cultivar IC8-B revealed higher reduction in shoot water content, hence, could be suggested more sensitive than IC8 and NC2 cultivars. Consequently, the relationship concerning plant water status and tolerance to Cd had been reported by Belimov et al [46] in pea mutant SGECd. In plant tissues, the transportation of water is reduced by Cd stress, hence, decreased the transpiration rate, stomatal conductance, WC and resulted in water stress [39,44].…”

Section: Water Relationmentioning

confidence: 71%

Comparative Study of Growth, Cadmium Accumulation and Tolerance of Three Chickpea (Cicer arietinum L.) Cultivars

Ullah

Khan

Hayat

et al. 2020

Plants

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Trace metals (TM) contamination is a severe problem in the environment and produced an adverse effect on the productivity of crops. Cadmium (Cd) is a TM ranked seven among the top 20 pollutants due to its high toxicity and solubility in water, taken up by the plants and affects their growth and metabolism. In this study, we evaluated the growth, Cd accumulation and tolerance capacities of three chickpea (Cicer arietinum L.) cultivars (NC234 (NC2), ICCV89310 (IC8) and ICCV89323-B (IC8-B)), subjected to two Cd concentrations (25 and 50 µM) in hydroponic culture. The toxicity of Cd reduced the plant height and fresh and dry biomass in all cultivars. The maximum reduction was observed at 50 µM of Cd. Compared with IC8-B, cultivars IC8 and NC2 exhibited better performance with high growth, biomass, root to shoot (R/S) ratio and water content under high Cd stress. To measure the accumulation of Cd in root and shoot, an inductively coupled plasma optical emission spectrometer (ICP-OES) was used. IC8 and NC2 had comparatively high Cd tolerance and accumulation ability (> 100 µg g−1 dry weight), with IC8 being more tolerant and accumulated higher Cd in shoot than NC2, while cultivar IC8-B was sensitive. Root accumulated more Cd than shoot in a dose-dependent manner. The bioconcentration factors (BCF) and bioaccumulation coefficients (BAC) were far higher than one (> 1) and increased with an increase in Cd concentrations, while the translocation factor (TF) was less than one (< 1), suggesting that all the three cultivars were unable to transfer Cd from the root to the shoot efficiently. Our results indicated that IC8 and NC2 proved to be resistant, while IC8-B showed sensitivity when exposed to high Cd stress (50 µM).

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Section: Water Relationmentioning

confidence: 71%

Comparative Study of Growth, Cadmium Accumulation and Tolerance of Three Chickpea (Cicer arietinum L.) Cultivars

Ullah

Khan

Hayat

et al. 2020

Plants

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“…The pea mutant SGECd t carries a recessive mutation in one gene and shows increased Cd tolerance and Cd accumulation (Tsyganov et al 2007). Although the mutated gene in SGECd t is still not found, the proposed mechanisms involved in its Cd tolerance were related to maintenance of biochemical and nutrient homeostasis (Tsyganov et al 2007, Belimov et al 2016, water relations (Belimov et al 2015) and proper root growth and function (Belimov et al 2018) under Cd stress. Therefore, an important next step was to study the effects of Cd on plant photosynthesis using this pea mutant and to determine its photosynthetic activity in order to better understand the mechanisms involved in plant response to toxic heavy metals.…”

Section: Introductionmentioning

confidence: 99%

Leaf nutrient homeostasis and maintenance of photosynthesis integrity contribute to adaptation of the pea mutant SGECd<sup>t</sup> to cadmium

Belimov¹,

Dodd²,

Safronova³

et al. 2020

Biologia plant.

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Cadmium (Cd) is a highly toxic and widespread soil pollutant, which negatively affects various aspects of plant growth and physiology. Here, the role of photosynthesis in response to Cd was investigated in the Cd-tolerant pea (Pisum sativum L.) mutant SGECd t . The wild type SGE and the mutant SGECd t were grown in a hydroponic solution supplemented with 1, 3, or 4 µM CdCl2 for 12 d. Root and shoot biomasses of the Cd-treated SGECd t were significantly higher than of SGE. Cadmium had little effect on the quantum yield of photosystem II (φPSII) and chlorophyll content of intact leaves of both pea genotypes. However, when leaf slices were taken from Cd-exposed plants and incubated with high Cd concentrations, the SGECd t mutant showed 1.5 -2 times higher φPSII values than SGE, with genotypic differences maximal at 0.1 and 1 mM CdCl2. In contrast, when leaf slices were taken from plants previously unexposed to Cd, both pea genotypes exhibited similar φPSII values. Cadmium content in leaves and mesophyll protoplasts of Cd-treated SGECd t were about 2 -3 times higher than in SGE ones. The mutant leaves and mesophyll protoplasts had also higher Ca, Mg, Mn, and Zn content. Thus, SGECd t acclimated to Cd during growth in the Cd-supplemented nutrient solution by developing a molecular mechanism related to photosynthetic integrity. A higher foliar nutrient content likely allows enhanced photosynthesis by counteracting the damage of leaves caused by Cd.

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“…Мутантная линия SGECd t несет рецессивную мутацию cdt [14], локализованную в VI группе сцепления гороха [15,16]. В результате прививочных экспериментов было показано, что генотип корня определяет повышенную устойчивость к кадмию и увеличенное его накопление в тканях мутантной линии [17].…”

Section: Introductionunclassified

Influence of mutation in pea (Pisum sativum L.) cdt (cadmium tolerance) gene on histological and ultrastructural nodule organization

Tsyganova

Викторовна²,

Seliverstova

et al. 2019

Ecological genetics

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Background. A comparative analysis out of the structural organization of the symbiotic nodules of the pea initial line SGE and the mutant line SGECdt, characterized by increased tolerance to cadmium and increased its accumulation, was carried out. Materials and methods.Nodules of initial line SGE and mutant SGECdt were analyzed using light and transmission electron microscopy. Results. The non-treated nodules of SGE and SGECdt were characterized by a similar histological and ultrastructural organization. In the nodules of SGE exposed to 100 M CdCl2 in infected cells, the following abnormalities were observed: expansion of the peribacteroid space, destruction of the symbiosome membrane, fusion of symbiosomes and, as a result, the formation of symbiosomes containing several bacteroids. In the nodules of SGECdt, infected cells did not undergo pronounced changes. In the nodules of SGE exposed to 1 mM CdCl2, at the base of the nodule, senescent infected cells with completely destroyed cytoplasm and degrading bacteroids appeared. Also there were present cells in which the contents of symbiosomes were lysing, and only the ghosts of the bacteroids remained in them. In SGECdt, in some infected cells, abnormalities were manifested in an increase in the peribacteroid space, partial destruction of symbiosome membranes, fusion of symbiosomes, and release of bacteroids into the vacuole. Conclusions. The tolerance of pea nodules to cadmium can be significantly increased due to a single recessive cdt mutation.

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The crucial role of roots in increased cadmium-tolerance and Cd-accumulation in the pea mutant SGECd<sup>t</sup>

Cited by 15 publications

References 45 publications

Comparative Study of Growth, Cadmium Accumulation and Tolerance of Three Chickpea (Cicer arietinum L.) Cultivars

Comparative Study of Growth, Cadmium Accumulation and Tolerance of Three Chickpea (Cicer arietinum L.) Cultivars

Leaf nutrient homeostasis and maintenance of photosynthesis integrity contribute to adaptation of the pea mutant SGECd<sup>t</sup> to cadmium

Influence of mutation in pea (Pisum sativum L.) cdt (cadmium tolerance) gene on histological and ultrastructural nodule organization

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