The crypt neurons in the olfactory epithelium of the adult zebrafish express TrkA-like immunoreactivity

Catania, Salvatore; Germanà, Antonino; Laurà, Rosaria; González-Martínez, T.; Ciriaco, E.; Vega, J.A.

doi:10.1016/s0304-3940(03)00751-1

Cited by 42 publications

(30 citation statements)

References 18 publications

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“…Reappearance of intense PHA-L staining in the ORC after day 56 may suggest that the same sugar residue becomes necessary again along with the differentiation of the ORC, because PHA-L staining is only moderate in the basal cells and undifferentiated ORC even at day 91. Late appearance of crypt cells at day 56 seems to suggest that these cells are necessary for the sophisticated function of the OE [3], because the first olfactory lamella extends to its full length and wholely covered with the OE at day 56 as reported in our previous study [17].…”

supporting

confidence: 66%

“…On the other hand, since putative pheromones are identified in some fish species [14,15], the olfactory function of the VNO may be taken charge of by the OE in fish. This presumption may be approved by the presence of three kinds of olfactory receptor cells (ORC) in the fish OE, that is, ciliated cells, microvillous cells and crypt cells [1,3,18]. Ciliated and microvillous cells are strongly supposed to differ in the property of odorant perception from each other by histochemical and electrophysiological studies [1,7,8,11].…”

mentioning

confidence: 99%

“…Since the ORC of the OE are ciliated and the ORC of the VNO are microvillous in general in the tetrapod [9], the general odorants may be perceived by the ciliated ORC and pheromonal molecules by the microvillous ORC in fish. In addition, crypt cells are unique olfactory neurons, and their olfactory function remains obscure [3]. On the other hand, we revealed the presence of four kinds of ORC, that is, protein gene product 9.5 (PGP 9.5)-immunoreactive cells, calretinin (CR)-immunoreactive cells, Phaseolus vulgaris agglutinin-L (PHA-L)-reactive cells and crypt cells, in our previous report according to their difference in the immunohistochemical and lectin histochemical characteristics [13].…”

mentioning

confidence: 99%

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Differential Expression of Histochemical Characteristics in the Developing Olfactory Receptor Cells in a Flatfish, Barfin Flounder (<i>Verasper moseri</i>)

Yamamoto

Mori

Saito

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. Differentiation of the histochemical characteristics of the olfactory receptor cells (ORC) was examined by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and calretinin (CR) and lectin histochemistry for Phaseolus vulgaris agglutinin-L (PHA-L) in the developing olfactory epithelium (OE) of the barfin flounder. PGP 9.5 immunoreactivity was diffuse and CR immunoreactivity was restricted at day 7, but these immunoreactivities became intense in the OE toward day 91. Crypt cells were first identified at day 56. PHA-L staining was faint at day 28, but became intense toward day 91. These findings suggest that PGP 9.5-immunopositive c ells, CR-immunopositive cells, crypt cells and PHA-L-reactive cells differentiate independently in the developing OE and constitute subsets of the ORC in the OE. KEY WORDS: calretinin, Phaseolus vulgaris agglutinin-L, protein gene product 9.5.

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supporting

confidence: 66%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Differential Expression of Histochemical Characteristics in the Developing Olfactory Receptor Cells in a Flatfish, Barfin Flounder (<i>Verasper moseri</i>)

Yamamoto

Mori

Saito

et al. 2004

The Journal of Veterinary Medical Science

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“…function (Germana et al, 2004), the growth factor receptor Trk-A (Catania et al, 2003) and the G-proteins G o , G q and Gi 1b . G o was shown to strongly label crypt cells in channel catfish and goldfish, although it is also expressed in a subset of microvillous olfactory receptor neurons (Hansen et al, 2004;Hansen et al, 2003).…”

Section: A Bazáes and O Schmachtenbergmentioning

confidence: 99%

“…Crypt cells were detected with similar frequency in juvenile and adult specimens, but, unexpectedly, a significant portion of crypt cells were located at a depth within the olfactory epithelium without apparent access to the surface. Although several previous studies described a mostly apical localization of crypt cells in the olfactory epithelium and established this as a criterion for their identification (Catania et al, 2003;Ferrando et al, 2006;Hamdani and Døving, 2006;Hansen and Finger, 2000), another recent study reported that the apical position of crypt cells and their overall density depended on the spawning season (Hamdani et al, 2008). In contrast, studies in zebrafish, which express numerous crypt cells, failed to reveal a seasonal fluctuation of crypt cell density (Hansen and Finger, 2000), which might be related to the stable conditions of their natural habitat and their year-round reproductive activity (Spence et al, 2008).…”

Section: A Bazáes and O Schmachtenbergmentioning

confidence: 99%

Odorant tuning of olfactory crypt cells from juvenile and adult rainbow trout

Bazáes

Schmachtenberg

2012

Journal of Experimental Biology

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SUMMARYTeleost fish lack independent olfactory organs for odorant and pheromone detection. Instead, they have a single sensory epithelium with two populations of receptor neurons, ciliated and microvillous, that are conserved among vertebrates, and a unique receptor cell type named the olfactory crypt cell. Crypt cells were shown to be chemosensory neurons that project to specific areas in the olfactory bulb, but their odorant tuning and overall function remain unclear. Reproduction in fish is generally synchronized by sex pheromonal signaling between males and females, but the sensors responsible for pheromone detection remain unknown. In crucian carp, a seasonal variation in the population of olfactory crypt cells and their brain projections pathways, involved in reproduction, led to the hypothesis of a role as sex pheromone detectors. In the present study, morphology and localization of olfactory crypt cells were compared between juvenile and mature rainbow trout of both sexes, and calcium imaging was used to visualize responses of crypt cells from the three groups to common social and food-related odorants, sex hormones and conspecific tissue extracts. Crypt cells from mature trout were found to be larger than those of juvenile specimens, and preferentially localized to the apical surface of the olfactory epithelium. Although a fraction of crypt cells of all groups responded to common odorants such as amino acids and bile salts, cells from mature trout showed a characteristic preference for gonadal extracts and hormones from the opposite sex. These results support an involvement of olfactory crypt cells in reproduction-related olfactory signaling in fishes. Supplementary material available online at

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Appearance of Crypt Neurons in the Olfactory Epithelium of the Skate Raja clavata During Development

Ferrando

Bottaro

Pedemonte

et al. 2007

The Anatomical Record

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Crypt neurons are olfactory receptor cells located in the olfactory epithelium of fishes. They exhibit a peculiar and well-recognizable morphology, although their odorant specificity is still unknown. Data on their appearance during development are few and far between. This study set out to identify the time at which crypt neurons appeared in the skate, Raja clavata, using histological and immunohistochemical methods. For this purpose, embryos and juveniles at different stages of development, from 13 weeks after laying (11 weeks before hatching) to 24 weeks after hatching, were examined. The crypt neurons were identified on a morphological basis. An anti-a-tubulin antibody and two lectins (wheat germ agglutinin and peanut agglutinin) were used to highlight morphological details. The olfactory marker protein was detected by immunohistochemistry, because this protein is a marker of neuronal maturity in vertebrates. The crypt neurons could be detected by their morphology at 15 weeks after laying and became strongly olfactory marker protein immunoreactive 22 weeks after laying. Although involvement of crypt neurons in reproductive behavior has been inferred in various studies on bony fishes, their early presence in skate embryos and juveniles may suggest that they are not exclusively involved in sexual behavior. Anat Rec,

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The crypt neurons in the olfactory epithelium of the adult zebrafish express TrkA-like immunoreactivity

Cited by 42 publications

References 18 publications

Differential Expression of Histochemical Characteristics in the Developing Olfactory Receptor Cells in a Flatfish, Barfin Flounder (<i>Verasper moseri</i>)

Differential Expression of Histochemical Characteristics in the Developing Olfactory Receptor Cells in a Flatfish, Barfin Flounder (<i>Verasper moseri</i>)

Odorant tuning of olfactory crypt cells from juvenile and adult rainbow trout

Appearance of Crypt Neurons in the Olfactory Epithelium of the Skate Raja clavata During Development

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