The Crystallization of Hydroxyapatite in the Presence of Lysine

Koutsopoulos, Sotirios; Dalas, E.

doi:10.1006/jcis.2000.7144

Cited by 59 publications

(51 citation statements)

References 30 publications

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“…Their results indicate that the AAs occupy the vacant Ca and P sites of the growing HA (100) surfaces mainly through their amino and carboxylate groups, thus inhibiting HA growth along (100) direction. They showed that small and charged AAs, such as Gly, Glu and Asp, have a higher adsorption on HA, which was consistent with the experimental findings by Koutsapolous et al [20,22,64]. Pan et al [11] investigated the specific adsorption sites of Gly and Glu on the (100) and (001) faces of HA crystals at the atomic level using MD simulations.…”

Section: Amino Acids Bound To Surfacessupporting

confidence: 70%

The role of amino acids in hydroxyapatite mineralization

Tavafoghi¹,

Cerruti²

2016

J. R. Soc. Interface.

209

147

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Polar and charged amino acids (AAs) are heavily expressed in noncollagenous proteins (NCPs), and are involved in hydroxyapatite (HA) mineralization in bone. Here, we review what is known on the effect of single AAs on HA precipitation. Negatively charged AAs, such as aspartic acid, glutamic acid (Glu) and phosphoserine are largely expressed in NCPs and play a critical role in controlling HA nucleation and growth. Positively charged ones such as arginine (Arg) or lysine (Lys) are heavily involved in HA nucleation within extracellular matrix proteins such as collagen. Glu, Arg and Lys intake can also increase bone mineral density by stimulating growth hormone production. In vitro studies suggest that the role of AAs in controlling HA precipitation is affected by their mobility. While dissolved AAs are able to inhibit HA precipitation and growth by chelating Ca 2þ and PO 4 32 ions or binding to nuclei of calcium phosphate and preventing their further growth, AAs bound to surfaces can promote HA precipitation by attracting Ca 2þ and PO 4 32 ions and increasing the local supersaturation. Overall, the effect of AAs on HA precipitation is worth being investigated more, especially under conditions closer to the physiological ones, where the presence of other factors such as collagen, mineralization inhibitors, and cells heavily influences HA precipitation. A deeper understanding of the role of AAs in HA mineralization will increase our fundamental knowledge related to bone formation, and could lead to new therapies to improve bone regeneration in damaged tissues or cure pathological diseases caused by excessive mineralization in tissues such as cartilage, blood vessels and cardiac valves.

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Section: Amino Acids Bound To Surfacessupporting

confidence: 70%

The role of amino acids in hydroxyapatite mineralization

Tavafoghi¹,

Cerruti²

2016

J. R. Soc. Interface.

209

147

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“…Particular interest has been dedicated to amino acids, which are compounds of major importance for living organisms, even because their con- centration is controlled by physiological mechanisms and they enter inside the cell environment by simple diffusion [93,94]. The effects of 4-hydroxyproline, tyrosine and serine (amino acids with uncharged polar side groups), glycine, cysteine, cystine, and glutamine (amino acids with neutral polar side groups) and lysine, which is an amino acid with a basic side group, on HA crystallization have been studied by the ''constant composition technique'' [95][96][97]. Using this technique, the chemical potentials of the species in the working solution are kept constant during the crystallization process by the stoichiometric addition of reactants and therefore the crystal growth reaction is performed under pseudo-steady-state conditions.…”

Section: 6mentioning

confidence: 99%

Hydroxyapatite Nanocrystals as Bone Tissue Substitute

Roveri

Palazzo

2003

Nanotechnologies for the Life Sciences

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OverviewNanoscience, where the properties of materials are exploited to innovative amazing applications, is involved in the size-dependent chemical and biological activity of bone substitute materials, which is indeed a fascinating field. The present chapter reviews the synthesis and chemical-physical characteristics of hydroxyapatite (HA) nano-crystals, which have excellent properties to represent an elective material covering a wide range of applications for bone substitution. We start from an examination of biogenic bone and tooth hydroxyapatite nanocrystals morphological and chemical-physical characteristics. The highlighted concepts have been used to review up-to date main new ideas on the preparation of synthetic apatitic bone substitutes mimicking the above biogenic properties, among which the nanosize is the basis of their self-assembly, self-mineralization and bone regeneration ability.Hydroxyapatite (HA) nanocrystals with high bioreabsorbability containing foreign ions and mimicking bone HA chemical-physical and physiological behaviour have been described. We have pointed to their possible use in preparing scaffolds with a porosity simulating that of spongy bone and upon which cells can be seeded, thereby developing ''in vitro autologous bone''. Biologically inspired HA nanocrystals/collagen composites have been reviewed, focusing on the role of a self-assembling strategy in conditioning the bone repairing activity of this biomaterial. Furthermore, considering that calcium phosphate/collagen composites are not limited to loading application, the possibility of preparing bio-inspired coating on the surface of metallic implants could be an advantageous approach.Finally, surface functionalization of HA nano-crystals with bioactive molecules makes them able to transfer information and to act selectively on the biological environment and can be considered one of the main future challenges for innovative bone substitute materials. 283 7.2

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“…In the study of El Shafei et al, the results demonstrated a dependence on the side chain structure of amino acids and the configuration of the functional groups on the surface of HAP [3]. Koutsopoulos et al [4][5][6][7] reported that a Langmuir type of adsorption behavior was found for AA in the kinetic studies of HAP crystal growth. It was found that the crystal growth rates of HAP decrease markedly in the presence of both aspartic and glutamic acids [5].…”

Section: Introductionmentioning

confidence: 98%

Molecular dynamics simulations of the adsorption of amino acids on the hydroxyapatite {100}-water interface

Zhang

Pan

Tang

2008

Front. Mater. Sci. China

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The understanding of interfaces and interaction of organic molecules and inorganic materials are the important issues in biomineralization. Experimentally, it has been found that amino acids (AA) can regulate the morphology of hydroxyapatite (HAP) crystals significantly. In this study, molecular dynamics simulation is employed to investigate the detailed adsorption behavior of polar, ionic, and hydrophobic AA on the {100} face of HAP at the atomic level. The results indicate that various AA are adsorbed on the HAP crystal surface mainly by amino and carboxylate groups at the specific sites. Multiple interaction points are found for polar and ionic AA. The adsorbed AA molecules occupy the Ca and P sites of the HAP surfaces which may inhibit and regulate the HAP growth. The adsorbed amino acid layer can also change the interfacial hydration layer and influence the transportation of ions in and out of HAP, which may be another strategy of biological control in biomineralization.

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The Crystallization of Hydroxyapatite in the Presence of Lysine

Cited by 59 publications

References 30 publications

The role of amino acids in hydroxyapatite mineralization

The role of amino acids in hydroxyapatite mineralization

Hydroxyapatite Nanocrystals as Bone Tissue Substitute

Molecular dynamics simulations of the adsorption of amino acids on the hydroxyapatite {100}-water interface

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