The Crystallization of Liver Esterase.

Mohamed, M. Safwat

doi:10.3891/acta.chem.scand.02-0090

Cited by 13 publications

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“…The inhibitory action of the drug was tested on the bacterial esterase activity of the lving E. coli cells and on preparations of the bacterial esterase prepared according to the procedure of Tammisto (1933). The action of the antibiotic on bacterial esterase was then compared with its action on crystalline horse liver esterase (Mohamed, 1948) and mitochondria (Hogeboom et al, 1948). The suspensions of the bacterial cells used in these studies as the enzyme solutions were prepared by suspending the freshly harvested cells in 0.025 N NaHCO3 buffer, pH 7.4.…”

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Inhibition of Bacterial Esterases by Chloramphenicol (Chloromycetin)

1949

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Recently a series of experiments have been undertaken to determine the interrelationships between bacterial enzymes and the new antibiotic chloramphenicol. It was hoped that some inhibitory action of this drug on a given enzymatic system could be detected which might help to explain the mode of action of the compound on bacterial celLs. At the present time the inhibitory action of chloramphenicol has been tested on approximately 45 isolated enzymatic systems. The results obtained in our laboratory from these experiments have demonstrated that the antibiotic does not inhibit cellular respiration of either resting or actively growing bacterial cells. The utilization of carbohydrates with the production of organic acids by way of the phosphorylation cycle was also not blocked (Smith and Worrel, unpublished). Furthermore, the breakdown of proteins into amino acids and their tranmination were not influenced (Smith and Worrel, in press). These studies and related observations on the inhibitory action of chloramphenicol on phosphatases, nucleases, and oxidizing enzymes suggested the possibility that chloramphenicol might be inhibiting the metabolism of fats and related organic acid esters. Two possible types of reactions would be involved in this general metabolic process, namely, the hydrolysis of the fat or ester and the utilization of the resulting organic acid and alcohol. The present paper deals with a few of the observations made on the inhibitory action of chloramphenicol on bacterial esterases that are involved in the hydrolysis and synthesis of fatty acid esters.

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