The Csr/Rsm system of Yersinia and related pathogens

Heroven, Ann Kathrin; Böhme, Katja; Dersch, Petra

doi:10.4161/rna.19333

Cited by 91 publications

(86 citation statements)

References 119 publications

(177 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CsrA represses V. cholerae motility genes and activates genes for biofilm formation and cholera toxin (75). CsrA regulates genes necessary for the switch between early colonization and persistent infection in Y. pseudotuberculosis (4). Thus, the Csr system governs pivotal lifestyle decisions of Gammaproteobacteria, including the pathogens of this bacterial class.…”

Section: Discussionmentioning

confidence: 99%

“…These pathogens live both in environmental reservoirs and in a variety of animal hosts and are spread by fecal-oral contamination. Infections in humans result mainly from consumption of contaminated food, most commonly pork (4).…”

Section: Yersinia Speciesmentioning

confidence: 99%

“…At that time, examples of posttranscriptional regulation included the role of Hfq (then called host factor 1) in bacteriophage Q␤ replication and limited roles of ribosomal proteins in translation control (1,2). Hfq is now recognized as an RNA chaperone that promotes the interaction of numerous base-pairing small RNAs (sRNAs) with their mRNA targets and CsrA as a sequence-specific RNA binding protein, both of which globally influence gene expression and virulence (3)(4)(5)(6)(7)(8). Other posttranscriptional regulators also participate in bacterial virulence networks, including RNA helicases, ribonucleases, and the Crc protein of pseudomonads (9)(10)(11)(12)(13)(14)(15).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Regulation of Bacterial Virulence by Csr (Rsm) Systems

Vakulskas

Potts

Babitzke

et al. 2015

Microbiol Mol Biol Rev

302

400

View full text Add to dashboard Cite

SUMMARY Most bacterial pathogens have the remarkable ability to flourish in the external environment and in specialized host niches. This ability requires their metabolism, physiology, and virulence factors to be responsive to changes in their surroundings. It is no surprise that the underlying genetic circuitry that supports this adaptability is multilayered and exceedingly complex. Studies over the past 2 decades have established that the CsrA/RsmA proteins, global regulators of posttranscriptional gene expression, play important roles in the expression of virulence factors of numerous proteobacterial pathogens. To accomplish these tasks, CsrA binds to the 5′ untranslated and/or early coding regions of mRNAs and alters translation, mRNA turnover, and/or transcript elongation. CsrA activity is regulated by noncoding small RNAs (sRNAs) that contain multiple CsrA binding sites, which permit them to sequester multiple CsrA homodimers away from mRNA targets. Environmental cues sensed by two-component signal transduction systems and other regulatory factors govern the expression of the CsrA-binding sRNAs and, ultimately, the effects of CsrA on secretion systems, surface molecules and biofilm formation, quorum sensing, motility, pigmentation, siderophore production, and phagocytic avoidance. This review presents the workings of the Csr system, the paradigm shift that it generated for understanding posttranscriptional regulation, and its roles in virulence networks of animal and plant pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Yersinia Speciesmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Regulation of Bacterial Virulence by Csr (Rsm) Systems

Vakulskas

Potts

Babitzke

et al. 2015

Microbiol Mol Biol Rev

302

400

View full text Add to dashboard Cite

show abstract

“…Csr is a regulatory complex in which non-coding Csr RNAs titrate the binding of CsrA to mRNA targets, leading to degradation of these targets. The Csr system has pleiotropic effects on the expression levels of injectisome components in Salmonella [81,82], EPEC [83] and Yersinia (reviewed in [84]), and was recently shown to downregulate the injectisome in persistent Yersinia infections [85]. CsrA Table 2.…”

Section: Transcriptional and Post-transcriptional Regulation Of The T3ssmentioning

confidence: 99%

Type III secretion systems: the bacterial flagellum and the injectisome

Diepold

Armitage

2015

Phil. Trans. R. Soc. B

189

175

View full text Add to dashboard Cite

One contribution of 12 to a theme issue 'The bacterial cell envelope'. The flagellum and the injectisome are two of the most complex and fascinating bacterial nanomachines. At their core, they share a type III secretion system (T3SS), a transmembrane export complex that forms the extracellular appendages, the flagellar filament and the injectisome needle. Recent advances, combining structural biology, cryo-electron tomography, molecular genetics, in vivo imaging, bioinformatics and biophysics, have greatly increased our understanding of the T3SS, especially the structure of its transmembrane and cytosolic components, the transcriptional, post-transcriptional and functional regulation and the remarkable adaptivity of the system. This review aims to integrate these new findings into our current knowledge of the evolution, function, regulation and dynamics of the T3SS, and to highlight commonalities and differences between the two systems, as well as their potential applications.

show abstract

“…Therefore, CsrB and CsrC induce gene expression by sequestering CsrA and thus antagonizing its translational repression activity. Expression of csrB and csrC, which are located in different chromosomal regions, is transcriptionally regulated by the two-component system BarA/UvrY, where BarA is the sensor kinase protein and UvrY is the DNAbinding response regulator (7,8).…”

mentioning

confidence: 99%

In Silico Identification and Experimental Characterization of Regulatory Elements Controlling the Expression of the Salmonella csrB and csrC Genes

Martínez¹,

Martínez-Flores²,

Salgado³

et al. 2013

Journal of Bacteriology

View full text Add to dashboard Cite

The small RNAs CsrB and CsrC of Salmonella indirectly control the expression of numerous genes encoding widespread cellular functions, including virulence. The expression of csrB and csrC genes, which are located in different chromosomal regions, is coordinated by positive transcriptional control mediated by the two-component regulatory system BarA/SirA. Here, we identified by computational analysis an 18-bp inverted repeat (IR) sequence located far upstream from the promoter of Salmonella enterica serovar Typhimurium csrB and csrC genes. Deletion analysis and site-directed mutagenesis of the csrB and csrC regulatory regions revealed that this IR sequence is required for transcriptional activation of both genes. Protein-DNA and proteinprotein interaction assays showed that the response regulator SirA specifically binds to the IR sequence and provide evidence that SirA acts as a dimer. Interestingly, whereas the IR sequence was essential for the SirA-mediated expression of csrB, our results revealed that SirA controls the expression of csrC not only by binding to the IR sequence but also by an indirect mode involving the Csr system. Additional computational, biochemical, and genetic analyses demonstrated that the integration host factor (IHF) global regulator positively controls the expression of csrB, but not of csrC, by interacting with a sequence located between the promoter and the SirA-binding site. These findings contribute to the better understanding of the regulatory mechanism controlling the expression of CsrB and CsrC.

show abstract

The Csr/Rsm system of Yersinia and related pathogens

Cited by 91 publications

References 119 publications

Regulation of Bacterial Virulence by Csr (Rsm) Systems

Regulation of Bacterial Virulence by Csr (Rsm) Systems

Type III secretion systems: the bacterial flagellum and the injectisome

In Silico Identification and Experimental Characterization of Regulatory Elements Controlling the Expression of the Salmonella csrB and csrC Genes

Contact Info

Product

Resources

About