The culturable mycobiota associated with the Mediterranean sponges Aplysina cavernicola, Crambe crambe and Phorbas tenacior

Abstract

Marine fungi are part of the huge and understudied biodiversity hosted in the sea. To broaden the knowledge on fungi inhabiting the Mediterranean Sea and their role in sponge holobiont, three sponges namely Aplysina cavernicola, Crambe crambe and Phorbas tenacior were collected in Villefranche sur Mer, (France) at about 25 m depth. The fungal communities associated with the sponges were isolated using different techniques to increase the numbers of fungi isolated. All fungi were identified to species level giving rise to 19, 13 and 3 species for P. tenacior, A. cavernicola and C. crambe, respectively. Of note, 35.7% and 50.0% of the species detected were either reported for the first time in the marine environment or in association with sponges. The mini-satellite analysis confirmed the uniqueness of the mycobiota of each sponge, leading to think that the sponge, with its metabolome, may shape the microbial community.

Cultivable fungal diversity in two karstic caves in Italy: under-investigated habitats as source of putative novel taxa

“…Interestingly, the putative new taxa were isolated at 10 °C and did not grow at 25 °C. This underlines once again the importance of mimicking the natural environment in terms of temperature (10 °C) and/or oligotrophy (SNA medium), in order to optimise the recovery of the autochthonous mycoflora 38 . Had we applied standard methods (e.g.…”

New Phenylspirodrimanes from the Sponge-Associated Fungus Stachybotrys chartarum MUT 3308

“…Stachybotrys chartarum MUT 3308 was isolated from the marine sponge Aplysina cavernicola sampled in the Mediterranean Sea at Villefranche-sur-Mer, France (Lat: 43°41′31.48707839999″ N, Lon: 7°19′12.185658623999″ E) [ 31 ]. The fungus was isolated by direct plating of the sponge tissues on Corn Meal Agar Seawater (CMAS; corn meal 2 g, agar 15 g, sea salts mix 30 g, gentamicin sulfate 40 mg, piperacillin and tazobactam 11 mg, Sigma-Aldrich (Merck KGaA, Saint-Louis, MO, USA) up to 1 L DI H 2 O) after incubation at 15 °C.…”

“…In this context, we were interested by PSDs produced by the fungus Stachybotrys chartarum MUT 3308, previously isolated from the Mediterranean sponge Aplysina cavernicola [ 31 ] and cultivated in solid and liquid media. Herein, we report the isolation of two PSDs, never isolated before, stachybotrin J ( 1 ) and new stachybocin G ( epi -stachybocin A) ( 2 ), along with the already reported stachybotrin I ( 3 ), stachybotrin H ( 4 ), stachybotrylactam ( 5 ), stachybotrylactam acetate ( 6 ), 2 α -acetoxystachybotrylactam acetate ( 7 ), stachybotramide ( 8 ), chartarlactam B ( 9 ), and F1839-J ( 10 ) ( Figure 1 ).…”

Dihydroauroglaucin Isolated from the Mediterranean Sponge Grantia compressa Endophyte Marine Fungus Eurotium chevalieri Inhibits Migration of Human Neuroblastoma Cells

“…Endophytic marine fungi, especially those associated with sessile macroorganisms such as sponges [ 1 ], are promising sources of bioactive secondary metabolites. In the frame of our ongoing work on the valorisation of the biotechnological potential of marine fungi [ 2 ], we have recently reported the fungus Eurotium chevalieri MUT 2316, isolated from the Mediterranean sponge Grantia compressa , as a high-yield source of bioactive metabolites [ 3 , 4 ].…”