The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine

Tsung, Hsiao Chien; Du, Zhong Wei; Ren, Rui; Li, Xiu Lan; Bao, Lingzhi; Wu, Jun; Bao, Shi Min; Yao, Zhen

doi:10.1038/sj.cr.7290164

Cited by 34 publications

(30 citation statements)

References 22 publications

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“…Similarly, there are several reports of pig ES-like cell lines that were derived from the early genital ridge tissue of the pig. These concern the isolation and culture of the pluripotent primordial germ cells to establish so-called embryonic germ (EG) cell lines which, in the case of mouse and human EG cell lines, have proven to be or are assumed to be functionally equivalent to ES cell lines [32][33][34][35]. However, putative EG cell lines of ungulates are not the subject of this review and are only noted for the reader's information.…”

Section: Review Of Ungulate Es Cell Line Literaturementioning

confidence: 97%

The Pursuit of ES Cell Lines of Domesticated Ungulates

Talbot¹,

Blomberg²

2008

Stem Cell Rev

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In contrast to differentiated cells, embryonic stem cells (ESC) maintain an undifferentiated state, have the ability to self-renew, and exhibit pluripotency, i.e., they can give rise to most if not all somatic cell types and to the germ cells, egg and sperm. These characteristics make ES cell lines important resources for the advancement of human regenerative medicine, and, if established for domesticated ungulates, would help make possible the improvement of farm animals through their contribution to genetic engineering technology. Combining other genetic engineering technologies, such as somatic cell nuclear transfer with ESC technology may result in synergistic gains in the ability to precisely make and study genetic alterations in mammals. Unfortunately, despite significant advances in our understanding of human and mouse ESC, the derivation of ES cell lines from ungulate species has been unsuccessful. This may result from a lack of understanding of species-specific mechanisms that promote or influence cell pluripotency. Thorough molecular characterizations, including the elucidation of stem cell "marker" signaling cascade hierarchy, species-appropriate pluripotency markers, and pluripotency-associated chromatin alterations in the genomes of ungulate species, should improve the chances of developing efficient, reproducible technologies for the establishment of ES cell lines of economically important species like the pig, cow, goat, sheep and horse.

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Section: Review Of Ungulate Es Cell Line Literaturementioning

confidence: 97%

The Pursuit of ES Cell Lines of Domesticated Ungulates

Talbot¹,

Blomberg²

2008

Stem Cell Rev

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“…Thirdly, different from Yin et al, we found that EGFP was expressed in genital ridges. This result proved that pluripotent embryonic germ (EG) cells in rabbits, similar to those in mice [21] and pigs [30], are transmitted into genital ridges.…”

Section: Discussionmentioning

confidence: 72%

Establishment of a Rabbit Oct4 Promoter-Based EGFP Reporter System

et al. 2014

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Rabbits are commonly used as laboratory animal models to investigate human diseases and phylogenetic development. However, pluripotent stem cells that contribute to germline transmission have yet to be established in rabbits. The transcription factor Oct4, also known as Pou5f1, is considered essential for the maintenance of the pluripotency of stem cells. Hence, pluripotent cells can be identified by monitoring Oct4 expression using a well-established Oct4 promoter-based reporter system. This study developed a rabbit Oct4 promoter-based enhanced green fluorescent protein (EGFP) reporter system by transfecting pROP2-EGFP into rabbit fetal fibroblasts (RFFs). The transgenic RFFs were used as donor cells for somatic cell nuclear transfer (SCNT). The EGFP expression was detected in the blastocysts and genital ridges of SCNT fetuses. Fibroblasts and neural stem cells (NSCs) were derived from the SCNT fetuses. EGFP was also reactivated in blastocysts after the second SCNT, and induced pluripotent stem cells (iPSCs) were obtained after reprogramming using Yamanaka's factors. The results above indicated that a rabbit reporter system used to monitor the differentiating status of cells was successfully developed.

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“…The ability to direct the differentiation of hES cell lines into a population of a specific cell type provide an unlimited supply of different cell types for tissue replacement and drug screening. The characteristics of ES cells include positive staining of specific cell‐surface markers (SSEA‐3, SSEA‐4, Oct‐4, TRA‐1‐60 and TRA‐l‐81), high ALP and telomerase activities, as well as normal karyotyping and multi‐lineage differentiation potential 9 . Knockout serum replacement was used to maintain stable culture conditions and avoid cell differentiation.…”

Section: Discussionmentioning

confidence: 99%