The Culture of Mammalian Cells on Nanostructured Silicon

Abstract

RSiCl 3 : Freshly prepared wafers were placed into a solution of the appropriate trichlorosilane (5 mM) in freshly distilled toluene for 2 h. Upon removal from the toluene solution, the wafers were rinsed with chloroform and absolute ethanol. The wafers were then baked on a hot plate at 120 C for 2 min.Monolayer Characterization: Contact angles were determined on a Krüss Model G10 goniometer at room temperature and ambient relative humidity using the sessile drop method [21]. Monolayer thickness was determined using a Gaertner L116A ellipsometer equipped with a 632.8 nm He-Ne laser. Monolayer thicknesses were determined assuming n f = 1.5, and with the incident and reflected light making a 70 C angle to the surface normal. Data presented are averages of at least ten different measurements on three different areas of the wafer. Film roughness was determined in contact mode AFM and calculated using PSI ProScan software.

Porous silicon in drug delivery devices and materials☆

“…Because the body can handle and eliminate silicic acid, the important issue with porous Si-based drug delivery systems is the rate at which they degrade and resorb [12,14,15,59,60]. The work of Bayliss, Canham, and others established the relatively low toxicity of porous Si in various cellular and live animal systems [21,[61][62][63][64][65][66]. The low toxicity, degradation properties, and solubility of the degradation byproducts of porous Si have generated much interest in its use in controlled drug delivery systems.…”

Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles

“…One of the most interesting features of porous silicon is its longterm stability at low pH (<6.0) [5] and the high hydrophobic nature of its native surface that can be extremely advantageous for the adsorption and delivery of small hydrophobic molecules such as doxorubicin [6], dexamethasone [7] or porphyrins [8]. Although the biocompatibility status of mesoporous silicon is still under investigation, it is known that the bulk material has negligible cytotoxicity [9]. Similar positive results have been observed for mesoporous silicon microparticles particles with sizes above 10 μm [10].…”

Chemical and Biological Applications of Porous Silicon Technology

“…[54] Successful adhesion of Chinese hamster ovary cells [55] (CHO) and rat neuronal (B50) cells [56] on porous silicon has been carried out by Bayliss and co-workers in a simulated blood plasma environment which also contains protein (human serum albumin). In a comparison between porous silicon and other silicon-based substrates, porous silicon shows a higher viability for B50 cells (Fig.…”