1945
DOI: 10.1093/infdis/77.3.187
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The Culturing of Blood Clots for Brucella Organisms

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1951
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“…The procedures and interpretations that evolved during the course of our attempts to evaluate the extent of antibody inhibition in Brucella blood cultures have varied extensively since the work was initiated. Blood clots washed with saline were first employed, this procedure being similar to that recommended by West and Borman (1945) and Mickle (1949). It was then found that cultures became positive earlier when the broth was enriched with hemolyzed (distilled water) normal human erythrocytes.…”
Section: Discussionmentioning
confidence: 99%
“…The procedures and interpretations that evolved during the course of our attempts to evaluate the extent of antibody inhibition in Brucella blood cultures have varied extensively since the work was initiated. Blood clots washed with saline were first employed, this procedure being similar to that recommended by West and Borman (1945) and Mickle (1949). It was then found that cultures became positive earlier when the broth was enriched with hemolyzed (distilled water) normal human erythrocytes.…”
Section: Discussionmentioning
confidence: 99%
“…A ausência de aglutininas em doentes com hemoculturas positivas também já foi descrita. WEST e BORMAN(1945), cultivando sistemáticamente coágulos sanguíneos de indivíduos com moléstia febril, verificaram em 16% a presença de brucelas sem o achado de aglutininas no sôro. Suas verificações, entretanto, não puderam ser reproduzidas por outros autores (SPINK" (1952).…”
Section: Methodsunclassified