The current clinical and geographical situation of cutaneous leishmaniasis based on species identification in Turkey

Özbilgin, Ahmet; Töz, Seray; Harman, Mehmet; Topal, Suhan Günaştı; Uzun, Soner; Okudan, Fulya; Güngör, Dilek; Erat, Ayşegül; Ertabaklar, Hatice; Ertuğ, Sema; Gündüz, Cumhur; Çavuş, İbrahim; Karakuş, Mehmet; Ural, İpek Östan; Ölgen, M. Kirami; Kayabaşı, Çağla; Kurt, Özgür; Özbel, Yusuf

doi:10.1016/j.actatropica.2018.11.001

Cited by 37 publications

(34 citation statements)

References 31 publications

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“…However, confirmation of these hosts as reservoirs requires xenodiagnostic studies as performed in Italy and Spain for L. infantum (Maroli et al ., 2007; Jimenez et al ., 2014). Adana and Hatay provinces are highly endemic areas for CL caused by L. tropica and L. infantum (Serin et al ., 2005), and recently L. major and L. donovani were also reported as causative agents in both provinces (Koltas et al ., 2014; Özbilgin et al ., 2019). According to the Ministry of Health records, a total of 616 and 207 CL cases were reported from Adana and Hatay provinces between 2013 and 2017, respectively (Özbel Y, personal comm).…”

Section: Discussionmentioning

confidence: 99%

“…According to the Ministry of Health records, a total of 616 and 207 CL cases were reported from Adana and Hatay provinces between 2013 and 2017, respectively (Özbel Y, personal comm). In a recent study, 21 out of 25 strains (84%) from Adana province were detected as L. tropica , and there were only two isolates from Hatay province and one of them is also found as L. tropica by Realtime ITS1 PCR (Özbilgin et al ., 2019). Dog studies carried out in these provinces revealed a high prevalence of CanL as 27.18% by IFAT and 41.74% by conjunctival swap nested PCR in rural areas of Adana (Karakuş et al ., 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Leishmania infantum MON-1 is the main causative agent for zoonotic human and canine visceral leishmaniasis while anthroponotic L. tropica is the main species causing CL in all regions of Turkey. Besides these agents, L. tropica and L. donovani in VL patients while L. infantum , L. major and L. donovani in CL patients were also reported (Koltas et al ., 2014; Özbilgin et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

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First molecular detection and identification of Leishmania species in small wild rodents from Turkey

et al. 2020

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Leishmaniasis is a parasitic disease infecting animals and humans. Two clinical forms (Visceral and cutaneous leishmaniasis) and four species are reported to be present in Turkey. Several studies have investigated canine and human leishmaniasis in Turkey but no study was performed to screen the infection among wild rodents, so far. The present study aims to investigate the role of small wild rodents as reservoir animals for Leishmania spp. in different regions of Turkey. Formalin-preserved tissue samples (spleen, liver, lung) of 712 rodents from 30 provinces were screened for the presence of Leishmania spp. DNA. Before DNA extraction, tissues were dried, rehydrated, and homogenated. Leishmania screening in rodent tissues and species determination was performed with a combination of real-time kDNA and ITS1 polymerase chain reaction protocols. Eight (1.12%) out of 712 animals were found to be positive for Leishmania spp. DNA and species typing revealed five L. infantum, two L. tropica and one L. major among positives. Leishmania major and L. infantum DNA were detected in Apodemus spp. from Zonguldak province located in the Western Black Sea Region, while L. tropica DNA was found in Meriones sp. and Gerbillus dasyurus from Adana and Hatay provinces located in Eastern Mediterranean Region of Turkey. The present study is first to report natural infection of L. infantum, L. major and L. tropica in small wild rodents in Turkey, suggesting their possible roles as reservoirs. Further studies are needed for planning epidemiological studies and also for developing rodent control measures in risky endemic areas to break the transmission cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First molecular detection and identification of Leishmania species in small wild rodents from Turkey

et al. 2020

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“…Although L. tropica and L. major species are the most common reason of CL in Turkey, previous studies showed that L infantum causes CL as well ( 4 , 5 ).…”

Section: Introductionmentioning

confidence: 99%

The Investigation of the Association of Cutaneous Leishmaniasis in Biopsy Specimens of the Patients with Granulomatous Disease and Skin Cancer Using the Molecular Method

Çulha

Doğramacı

Hakverdı

et al. 2020

IJPA

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Background: Clinically, cutaneous leishmaniasis (CL) can be confused with granulomatous diseases and skin cancers, and it may lead to erroneous diagnosis and treatment. Diagnosis based and histopathology can have some difficulties due to low number of parasites, especially in chronic CL cases. We aimed to emphasize the necessity of considering CL in the differential diagnosis for cases of granulomatous diseases and basal cell carcinoma, particularly in areas where CL is endemic. Methods: One hundred and seven paraffin-embedded tissue biopsy specimens were selected from the archive, as of 2002, of Pathology Department, School of Medicine, University of Hatay Mustafa Kemal in Hatay, Turkey. After DNA isolation, performed with the samples were used for PCR analysis with specific 13A, 13B primers targeting kinetoplastid DNA (kDNA) found in all Leishmania species. Another PCR was performed with LITSR and L5.8S primers targeting ITS-1 internal-transcribed-spacer-1 (ITS-1) region to subtype positive samples. Then these samples were further analyzed for subtyping with PCR-RFLP using HaeIII enzyme (BsuRI). Results: Ten out of 107 tissue specimens were positive via kDNA-PCR. Lupus vulgaris, sarcoidosis, skin lymphoma and Leishmania cutis appeared in 9 out of 10 positive specimens. One of the cases presented with a mass on the cheek and was pre-diagnosed with hemangioma, but leishmaniasis did not appear. All of 10 specimens were diagnosed as granulomatous dermatitis. Two out of 10 samples, found positive with kDNA-PCR, were analyzed with ITS-1-PCR and identified as L. infantum/donovani after RFLP. Conclusion: Molecular methods should be utilized in the differential diagnosis of CL to eliminate false diagnoses of granulomatous diseases and skin cancers.

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“…in Turkey but recently cases caused by L. major and L. donovani have also been reported especially from Syrian refugees. [8][9][10] CL is the most common form of the leishmaniasis, and it is characterized by localized ulcerative self-healing skin lesions on exposed parts of the body leaving life-long scars. Currently, there is still no vaccine or an effective treatment for CL.…”

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confidence: 99%

Association of cytokine gene polymorphisms with susceptibility to cutaneous leishmaniasis in a Turkish population

Kırık

Ülger

et al. 2020

Parasite Immunology

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Human leishmaniasis is a vector-borne disease caused by intracellular protozoan parasite species belonging to the genus Leishmania (over 20 species), and it is transmitted to human by the bite of infected female phlebotomine sand fly (Phlebotomus spp. in the Old World and Lutzomyia spp. in the New World). 1,2 There are three main clinical forms of Leishmaniasis: cutaneous leishmaniasis (CL), mucocutaneous leishmaniasis (MCL) and visceral leishmaniasis (VL). 3 According to the World Health Organization (WHO) fact sheet data, 97 countries are endemic for leishmaniasis and an estimated 700.000-1 million new cases and 26.000-65.000 deaths occur worldwide, annually. An estimated 50.000 to 90.000 new cases of VL occur worldwide each year. In 2017, more than 95% of new VL cases occurred in Bangladesh,

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The current clinical and geographical situation of cutaneous leishmaniasis based on species identification in Turkey

Cited by 37 publications

References 31 publications

First molecular detection and identification of Leishmania species in small wild rodents from Turkey

First molecular detection and identification of Leishmania species in small wild rodents from Turkey

The Investigation of the Association of Cutaneous Leishmaniasis in Biopsy Specimens of the Patients with Granulomatous Disease and Skin Cancer Using the Molecular Method

Association of cytokine gene polymorphisms with susceptibility to cutaneous leishmaniasis in a Turkish population

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