As the rapid development of renewable energy, energy sectors have to increase the load transfer capacity of transmission line. In order to calculate the line ampacity accurately, it is necessary to establish a valid mathematical model for line AC resistance. However, the simplified AC resistance model, which is current commonly used, does not take into account the nonlinear impact of current variation. In this paper, an modified AC resistance model regarding current-carrying variation is proposed, and the parameters in the proposed model are estimated by the experiment data. Combining IEEE standard and proposed model, the ampacity of conductor LGJ-120/25 is calculated and analyzed. The calculated results indicates that the ampacity obtained by proposed model is less than ampacity obtained by simplified AC resistance model. That means the ampacity would be overestimated and the security of transmission line would be reduced when the nonlinear impact of current variation is ignored.