The current state of microbial proteomics: Where we are and where we want to go

Chao, Tzu-Chiao; Hansmeier, Nicole

doi:10.1002/pmic.201100381

Cited by 20 publications

(8 citation statements)

References 137 publications

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“…[21], and so on. This method could not properly elucidate how resistant bacteria live and operate in their host systems [22]. This method was limited in scope because the procedure cannot detect biological phenotypes from the pathways of gene biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis reveals drug resistance of Staphylococcus xylosus ATCC700404 under tylosin stress

et al. 2019

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Background As a kind of opportunist pathogen, Staphylococcus xylosus (S. xylosus) can cause mastitis. Antibiotics are widely used for treating infected animals and tylosin is a member of such group. Thus, the continuous use of antibiotics in dairy livestock enterprise will go a long way in increasing tylosin resistance. However, the mechanism of tylosin-resistant S. xylosus is not clear. Here, isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics methods was used to find resistance-related proteins. Results We compared the differential expression of S. xylosus in response to tylosin stress by iTRAQ. A total of 155 proteins (59 up-regulated, 96 down-regulated) with the fold-change of >1.2 or <0.8 ( p value ≤0.05) were observed between the S. xylosus treated with 1/2 MIC (0.25 μg/mL) tylosin and the untreated S. xylosus . Bioinformatic analysis revealed that these proteins play important roles in stress-response and transcription. Then, in order to verify the relationship between the above changed proteins and mechanism of tylosin-resistant S. xylosus , we induced the tylosin-resistant S. xylosus , and performed quantitative PCR analysis to verify the changes in the transcription proteins and the stress-response proteins in tylosin-resistant S. xylosus at the mRNA level . The data displayed that ribosomal protein L23 ( rplw ), thioredoxin( trxA ) and Aldehyde dehydrogenase A( aldA-1 ) are up-regulated in the tylosin-resistant S. xylosus , compared with the tylosin-sensitive strains. Conclusion Our findings demonstrate the important of stress-response and transcription in the tylosin resistance of S. xylosus and provide an insight into the prevention of this resistance, which would aid in finding new medicines . Electronic supplementary material The online version of this article (10.1186/s12917-019-1959-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis reveals drug resistance of Staphylococcus xylosus ATCC700404 under tylosin stress

et al. 2019

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“…During an infection, pathogenic bacteria can rapidly alter their proteome composition to adapt to hostile environments and evade immune response1234. How the bacteria regulate their proteome composition in vivo to accomplish host environment adaption and immune response evasion is, however, still unclear.…”

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confidence: 99%

Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes

et al. 2012

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Selected reaction monitoring mass spectrometry (SRM-MS) is a targeted proteomics technology used to identify and quantify proteins with high sensitivity, specificity and high reproducibility. Execution of SRM-MS relies on protein-specific SRM assays, a set of experimental parameters that requires considerable effort to develop. Here we present a proteome-wide SRM assay repository for the gram-positive human pathogen group A Streptococcus. Using a multi-layered approach we generated SRM assays for 10,412 distinct group A Streptococcus peptides followed by extensive testing of the selected reaction monitoring assays in >200 different group A Streptococcus protein pools. Based on the number of SRM assay observations we created a rule-based selected reaction monitoring assay-scoring model to select the most suitable assays per protein for a given cellular compartment and bacterial state. The resource described here represents an important tool for deciphering the group A Streptococcus proteome using selected reaction monitoring and we anticipate that concepts described here can be extended to other pathogens.

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“…Proteomics methods are making unprecedented contributions to the study of bacterial virulence factors [76,77]. Application of quantitative proteomic methods to microbial proteins allows for organism-level determination of molecules which are expressed in virulent but not nonvirulent strains of a bacterium.…”

Section: Proteomics Studies On Bacterial Infections Of the Nervous Symentioning

confidence: 99%

Application of “Omics” Technologies for Diagnosis and Pathogenesis of Neurological Infections

Noorbakhsh

Aminian

Power

2015

Curr Neurol Neurosci Rep

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Infections of the human nervous system have substantial morbidity and mortality but also represent among the most challenging of all neurological diseases because of the difficulty in establishing a diagnosis and implementing effective therapies. Neurological infections lead to altered expression levels of a wide range of host- and pathogen-derived biomolecules both within and outside of the nervous system. Quantitative analyses of these biomolecular perturbations have been traditionally performed using "classical" molecular or analytical methods, which evaluate one or few genes or their products at a time. Recent technical developments together with the increasing availability of high-throughput/content methodologies have enabled a more comprehensive overview of these molecular alterations and thus provide new approaches to the diagnosis and/or treatment of this group of disorders. Herein, we will review recent evidence pointing to the capacity of the so-called omics techniques in studying the nervous system infections with an emphasis on genomics, transcriptomics, and proteomics technologies.

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The current state of microbial proteomics: Where we are and where we want to go

Cited by 20 publications

References 137 publications

Comparative proteomic analysis reveals drug resistance of Staphylococcus xylosus ATCC700404 under tylosin stress

Comparative proteomic analysis reveals drug resistance of Staphylococcus xylosus ATCC700404 under tylosin stress

Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes

Application of “Omics” Technologies for Diagnosis and Pathogenesis of Neurological Infections

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