The Current State of Platelet and Granulocyte Cryopreser Vation

Valeri, C. R.

doi:10.3109/10408368109105862

Cited by 14 publications

(16 citation statements)

References 279 publications

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“…Previous studies at our laboratory have shown that by controlling the volume and geometry of the freezing container, it is possible to achieve a freezing rate of 2 to 3°C/min by storage in a -80°C mechanical freezer [27], Results were similar whether the pool of platelets frozen contained platelets from indi vidual units of blood or platelets isolated by mechanical apheresis procedures. There was no sign of bacterial contamination during platelet pooling.…”

Section: Stability Of 27% Dmso-saline Solution During Storage At Roomsupporting

confidence: 54%

“…After thawing, the DMSO was removed from the platelets by a dilutioncentrifugation wash procedure. Washed platelets can be resuspended in plasma and stored at room temperature for as long as 4 h prior to transfusion [27] This paper reports the effects of cryopreservation and frozen storage for as long as 3 years, and of shipment of the frozen platelets in polystyrene foam containers in dry ice.…”

Section: Introductionmentioning

confidence: 99%

“…Over the past 12 years the Naval Blood Research Laboratory has been evaluating the preservation of human platelets frozen with 6% dimethyl sulfoxide (DMSO) and storage at -80°C [26,27]. In the studies reported here, platelets were isolated either from indi vidual units of whole blood or from the donor by mechanical apheresis using the Haemonetics blood processor 30, the IBM blood pro cessor 2997 or the Fenwal CS-3000 blood processor [14].…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of Human Platelets Using 6% Dimethyl Sulfoxide and Storage at -80°C

Meleragno¹,

Carciero²,

Feingold³

et al. 1985

Vox Sanguinis

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Platelet studies were done in healthy male volunteers and in thrombocytopenic patients. Some of the platelets used in the study were isolated by mechanical apheresis using either the Haemonetics blood processor 30, the IBM blood processor 2997 or the Fenwal CS-3000 blood processor before freezing. Other platelets were isolated from individual units of whole blood and pooled before freezing. The platelets were frozen with a 6% cryoprotectant (DMSO) in a polyvinylchloride (PVC) plastic bag or a polyolefin plastic bag at -80°C in a mechanical freezer and stored for as long as 3 years. Some of the frozen platelets were transported in dry ice in polystyrene foam containers to determine whether they would be adversely affected by such treatment. Platelet recovery after freezing, thawing and washing was about 75%. In the healthy male volunteers, in vivo recovery of autologous platelets 1 - 2 h after transfusion was about 33%, and the life span was about 8 days. In the thrombocytopenic patients, in vivo recovery values were 50% of those from fresh platelets. The transfusion of previously frozen washed platelets reduced clinical bleeding in the thrombocytopenic patients with bleeding. There was no evidence of quality deterioration in platelets after storage at -80°C for at least 2 years, as determined from in vitro recovery and in vivo survival values, nor was there any adverse effect as a result of shipment of the frozen platelets in dry ice in polystyrene foam containers from one facility to another.

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Section: Stability Of 27% Dmso-saline Solution During Storage At Roomsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cryopreservation of Human Platelets Using 6% Dimethyl Sulfoxide and Storage at -80°C

Meleragno¹,

Carciero²,

Feingold³

et al. 1985

Vox Sanguinis

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“…Frozen platelet adhesion was significantly decreased in relation to fresh platelets and platelets that had been stored for 5 days [12]. Recovery, survival [13] and other in vitro function markers, such as stimulus-response coupling [14], aggregation [15], granule release and pH were also impaired in frozen platelets. However, an acceptable life span and a superior in vivo immediate haemostatic function of frozen platelets have been reported in animal [16] and human [17] research.…”

Section: Discussionmentioning

confidence: 91%

In vitro thromboelastometric evaluation of the efficacy of frozen platelet transfusion

Pérez-Ferrer

Suay

Viejo-Llorente

et al. 2015

Thrombosis Research

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“…Frozen platelet adhesion is significantly decreased when compared to both fresh platelets and platelets stored for >5 days [21]. Recovery, survival, and other in vitro function markers, such as stimulus-response coupling, aggregation, granule release, and pH, are also impaired in frozen platelets [22,23,24]. It has also recently been reported that frozen and thawed platelets showed reduced surface expression of GPIIb and GPIbα and diminished aggregation response to agonists [25].…”

Section: Discussionmentioning

confidence: 99%

Generation of Platelet Microparticles after Cryopreservation of Apheresis Platelet Concentrates Contributes to Hemostatic Activity

Eker¹,

Çeti̇nkaya²,

Pekel³

et al. 2017

Tjh

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Objective:In the last decade, substantial evidence has accumulated about the use of cryopreserved platelet concentrates, especially in trauma. However, little reference has been made in these studies to the morphological and functional changes of platelets. Recently platelets have been shown to be activated by cryopreservation processes and to undergo procoagulant membrane changes resulting in the generation of platelet-derived microparticles (PMPs), platelet degranulation, and release of platelet-derived growth factors (PDGFs). We assessed the viabilities and the PMP and PDGF levels of cryopreserved platelets, and their relation with thrombin generation.Materials and Methods:Apheresis platelet concentrates (APCs) from 20 donors were stored for 1 day and cryopreserved with 6% dimethyl sulfoxide. Cryopreserved APCs were kept at -80 °C for 1 day. Thawed APCs (100 mL) were diluted with 20 mL of autologous plasma and specimens were analyzed for viabilities and PMPs by flow cytometry, for thrombin generation by calibrated automated thrombogram, and for PDGFs by enzyme-linked immunosorbent assay testing.Results:The mean PMP and PDGF levels in freeze-thawed APCs were significantly higher (2763±399.4/µL vs. 319.9±80.5/µL, p<0.001 and 550.9±73.6 pg/mL vs. 96.5±49 pg/mL, p<0.001, respectively), but the viability rates were significantly lower (68.2±13.7% vs. 94±7.5%, p<0.001) than those of fresh APCs. The mean endogenous thrombin potential (ETP) of freeze-thawed APCs was significantly higher than that of the fresh APCs (3406.1±430.4 nM.min vs. 2757.6±485.7 nM.min, p<0.001). Moreover, there was a significant positive poor correlation between ETP levels and PMP levels (r=0.192, p=0.014).Conclusion:Our results showed that, after cryopreservation, while levels of PMPs were increasing, significantly higher and earlier thrombin formation was occurring in the samples analyzed despite the significant decrease in viability. Considering the damage caused by the freezing process and the scarcity of evidence for their in vivo superiority, frozen platelets should be considered for use in austere environments, reserving fresh platelets for prophylactic use in blood banks.

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The Current State of Platelet and Granulocyte Cryopreser Vation

Cited by 14 publications

References 279 publications

Cryopreservation of Human Platelets Using 6% Dimethyl Sulfoxide and Storage at -80°C

Cryopreservation of Human Platelets Using 6% Dimethyl Sulfoxide and Storage at -80°C

In vitro thromboelastometric evaluation of the efficacy of frozen platelet transfusion

Generation of Platelet Microparticles after Cryopreservation of Apheresis Platelet Concentrates Contributes to Hemostatic Activity

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