The Current Status and Future Applications of Hairy Root Cultures

Dhiman, Nisha; Patial, Vanita; Bhattacharya, Amita

doi:10.1007/978-981-13-0535-1_5

Cited by 17 publications

(13 citation statements)

References 268 publications

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“…In vitro they exhibit fast growth, are genetically stable, and can accumulate the same secondary metabolites as the parent plant. Hairy roots of over 100 medicinal plants have been investigated so far (see for example (Dhiman et al 2018), and a few of the most recent studies are reviewed below.…”

Section: Production Via Plant In Vitro Culturesmentioning

confidence: 99%

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Wawrosch

Zotchev

2021

Appl Microbiol Biotechnol

126

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Medicinal plants have been used by mankind since ancient times, and many bioactive plant secondary metabolites are applied nowadays both directly as drugs, and as raw materials for semi-synthetic modifications. However, the structural complexity often thwarts cost-efficient chemical synthesis, and the usually low content in the native plant necessitates the processing of large amounts of field-cultivated raw material. The biotechnological manufacturing of such compounds offers a number of advantages like predictable, stable, and year-round sustainable production, scalability, and easier extraction and purification. Plant cell and tissue culture represents one possible alternative to the extraction of phytochemicals from plant material. Although a broad commercialization of such processes has not yet occurred, ongoing research indicates that plant in vitro systems such as cell suspension cultures, organ cultures, and transgenic hairy roots hold a promising potential as sources for bioactive compounds. Progress in the areas of biosynthetic pathway elucidation and genetic manipulation has expanded the possibilities to utilize plant metabolic engineering and heterologous production in microorganisms. This review aims to summarize recent advances in the in vitro production of high-value plant secondary metabolites of medicinal importance.Key points• Bioactive plant secondary metabolites are important for current and future use in medicine• In vitro production is a sustainable alternative to extraction from plants or costly chemical synthesis• Current research addresses plant cell and tissue culture, metabolic engineering, and heterologous production Graphical abstract

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Section: Production Via Plant In Vitro Culturesmentioning

confidence: 99%

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Wawrosch

Zotchev

2021

Appl Microbiol Biotechnol

126

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“…Elicitors are the biotic or abiotic molecules which belong to several classes of compounds, do not share a common chemical structure, and can induce or enhance the biosynthesis of specific SM . Various parameters, such as elicitor type, concentrations, duration of exposure, treatment schedule, culture type, cell line, medium composition, presence or absence of growth regulation, and age or stage of the culture at the time of elicitor treatment are the major factors that can determine the effectiveness of the elicitation strategies on biomass and SM production .…”

Section: Introductionmentioning

confidence: 99%

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Halder

Sarkar

Jha

2019

Engineering in Life Sciences

238

116

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Elicitation is a possible aid to overcome various difficulties associated with the large‐scale production of most commercially important bioactive secondary metabolites from wild and cultivated plants, undifferentiated or differentiated cultures. Secondary metabolite accumulation in vitro or their efflux in culture medium has been elicited in the undifferentiated or differentiated tissue cultures of several plant species by the application of a low concentration of biotic and abiotic elicitors in the last three decades. Hairy root cultures are preferred for the application of elicitation due to their genetic and biosynthetic stability, high growth rate in growth regulator‐free media, and production consistence in response to elicitor treatment. Elicitors act as signal, recognized by elicitor‐specific receptors on the plant cell membrane and stimulate defense responses during elicitation resulting in increased synthesis and accumulation of secondary metabolites. Optimization of various parameters, such as elicitor type, concentration, duration of exposure, and treatment schedule is essential for the effectiveness of the elicitation strategies. Combined application of different elicitors, integration of precursor feeding, or replenishment of medium or in situ product recovery from the roots/liquid medium with the elicitor treatment have showed improved accumulation of secondary metabolites due to their synergistic effect. This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.

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“…Different factors can affect the function of the elicitor (biomass and secondary metabolite production) such as concentrations, type, duration of exposure, culture, stage of the culture, and medium composition (Kaur 2018; Dhiman et al 2018;Naik and Al-Khayri 2016). Previous researchers have reported that a combination of more than one elicitor in different culture media can increase plant yields more compared to a single elicitor (Wang et al 2009;Zheng et al 2008;Gangopadhyay et al 2011;Ramezani et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Physio-chemical responses of exogenous calcium nanoparticle and putrescine polyamine in Saffron (Crocus sativus L.)

Badihi¹,

Gerami²,

Akbarinodeh

et al. 2021

Physiol Mol Biol Plants

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The aim of this study was to investigate the effect of calcium nanoparticles (CaNP) and putrescine polyamine on some physiological and biochemical properties of saffron (Crocus sativus L.) under the control condition. Saffron corm was treated by different concentrations of putrescine (0, 0.25, 0.5, 1, 2 mM) and CaNP (0, 0.25, 0.5, 1, 1.5 g/l). The treatment of corm with putrescine and CaNP separately caused a significant increase in morphological parameters. Changes in biochemical parameters were also significant. Compared to other concentrations, the highest concentration of putrescine (1 mM) and CaNP (1 g/l) treatment in the plant showed the greatest effect. The combined effect of putrescine and CaNP treatment on morphological parameters was significant. The results of HPLC analysis showed that CaNP treatment alone is more effective on crocin, picrocrocin, and safranal content than the combined effect of CaNP and putrescine. The present study reported the functional potential of CaNP and putrescine combination to increase growth and phytochemical properties in Crocus sativus.

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The Current Status and Future Applications of Hairy Root Cultures

Cited by 17 publications

References 268 publications

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Physio-chemical responses of exogenous calcium nanoparticle and putrescine polyamine in Saffron (Crocus sativus L.)

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