The current status and trend of the functional magnetic resonance combined with stimulation in animals

Huang, Jiayan; Zhang, Yusi; Zhang, Qi; Wei, Linxuan; Zhang, Xiwen; Jin, Caiping; Yang, Junchao; Li, Zuanfang; Liang, Shengxiang

doi:10.3389/fnins.2022.963175

Cited by 4 publications

(1 citation statement)

References 153 publications

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“…Recommendations and good practices for fMRI studies were summarized by different groups ( Schwarz et al, 2011a ; Schwarz et al, 2011b ; Mandeville et al, 2014 ; Khalili-Mahani et al, 2017 ). Performing brain fMRI studies in small rodents poses additional challenges, related not only to data acquisition and analysis but also to anesthesia ( Pan et al, 2015 ; Chuang and Nasrallah, 2017 ; Sumiyoshi et al, 2019 ; Huang et al, 2022 ). Although patterns of resting-state functional connectivity have been shown to be present in humans under anesthesia ( Greicius et al, 2008 ) or during the early stages of sleep ( Larson-Prior et al, 2009 ), great care needs to be taken when performing brain functional in anesthetized small rodents.…”

Section: Introductionmentioning

confidence: 99%

Magnetic resonance imaging and ultrasound elastography in the context of preclinical pharmacological research: significance for the 3R principles

et al. 2023

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The 3Rs principles—reduction, refinement, replacement—are at the core of preclinical research within drug discovery, which still relies to a great extent on the availability of models of disease in animals. Minimizing their distress, reducing their number as well as searching for means to replace them in experimental studies are constant objectives in this area. Due to its non-invasive character in vivo imaging supports these efforts by enabling repeated longitudinal assessments in each animal which serves as its own control, thereby enabling to reduce considerably the animal utilization in the experiments. The repetitive monitoring of pathology progression and the effects of therapy becomes feasible by assessment of quantitative biomarkers. Moreover, imaging has translational prospects by facilitating the comparison of studies performed in small rodents and humans. Also, learnings from the clinic may be potentially back-translated to preclinical settings and therefore contribute to refining animal investigations. By concentrating on activities around the application of magnetic resonance imaging (MRI) and ultrasound elastography to small rodent models of disease, we aim to illustrate how in vivo imaging contributes primarily to reduction and refinement in the context of pharmacological research.

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Section: Introductionmentioning

confidence: 99%

Magnetic resonance imaging and ultrasound elastography in the context of preclinical pharmacological research: significance for the 3R principles

et al. 2023

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The impact of isoflurane anesthesia on brain metabolism in mice: An MRI and electroencephalography study

Wei,

Roh,

Yang

et al. 2024

NMR in Biomedicine

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Isoflurane is one of the most widely used anesthetic agents in rodent imaging studies. However, the impact of isoflurane on brain metabolism has not been fully characterized to date, primarily due to a scarcity of noninvasive technologies to quantitatively measure the brain's metabolic rate in vivo. In this study, using noncontrast MRI techniques, we dynamically measured cerebral metabolic rate of oxygen (CMRO2) under varying doses of isoflurane anesthesia in mice. Concurrently, systemic parameters of heart and respiration rates were recorded alongside CMRO2. Additionally, electroencephalogram (EEG) recording was used to identify changes in neuronal activities under the same anesthetic regimen employed in the MRI experiments. We found suppression of the CMRO2 by isoflurane in a dose‐dependent manner, concomitant with a diminished high‐frequency EEG activity. The degree of metabolic suppression by isoflurane was strongly correlated with the respiration rate, which offers a potential approach to calibrate CMRO2 measurements. Furthermore, the metabolic level associated with neural responses of the somatosensory and motor cortices in mice was estimated as 308.2 μmol/100 g/min. These findings may facilitate the integration of metabolic parameters into future studies involving animal disease models and anesthesia usage.

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The mechanism of action of neuromodulation in the treatment of overactive bladder

Krhut,

Kobberø,

Kanaan

et al. 2024

Nat Rev Urol

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The current status and trend of the functional magnetic resonance combined with stimulation in animals

Cited by 4 publications

References 153 publications

Magnetic resonance imaging and ultrasound elastography in the context of preclinical pharmacological research: significance for the 3R principles

Magnetic resonance imaging and ultrasound elastography in the context of preclinical pharmacological research: significance for the 3R principles

The impact of isoflurane anesthesia on brain metabolism in mice: An MRI and electroencephalography study

The mechanism of action of neuromodulation in the treatment of overactive bladder

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